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Method of glycerolysis reaction for preparing partial glyceride

A partial glyceride and glycerol hydrolysis technology, applied in the field of oil production rich in partial glyceride, can solve problems such as increasing cost, and achieve the effects of avoiding hydrolysis, simplifying recovery and high thermal stability

Active Publication Date: 2013-04-24
YHSN TECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent US6337414B1 provides an enzymatic reaction method for adding water to the reaction system to successfully realize glycerololysis. Since water is added in the method, hydrolysis reaction also occurs, and a large amount of free fatty acids are produced in the product, which increases the separation of side reaction products and The cost of subsequent refining of the product

Method used

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  • Method of glycerolysis reaction for preparing partial glyceride

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Get 500g of activated macroporous weakly polar adsorption resin AB-8, 1000g of Tris-HCl buffer (concentration 0.05mol / L, pH8.8), 20g of free lipase Lipase T1 (Leow TC, RahmanRNZRA, Basri M, Salleh AB. High level expression of thermostable lipase from Geobacillus sp.strain T1[J]. Bioscience, biotechnology, and biochemistry 2004; 68(1): 96-103.) mixed, stirred and adsorbed at 30°C for 1 hour, It was then filtered and vacuum-dried to a moisture content of 0.5% to obtain lipase T1 immobilized on a macroporous adsorption resin, which was stored in a refrigerator at 4°C for later use. 9Kg refined soybean oil and 1Kg glycerin are added in raw material tank 1, and the moisture content in the control reaction system is no more than 0.1%, under the situation of magnetic stirring, as figure 1As shown, the reactant is transported by the pump 4 and flows through the enzyme reactor 5 and then returns to the raw material tank 1. The amount of immobilized lipase LipaseT1 is 300 g, the ...

Embodiment 2

[0026] Take 500g of activated macroporous weakly polar adsorption resin AB-8, 1000g of Tris-HCl buffer solution (concentration 0.05mol / L, pH8.8), 20g of free lipase Lipase T1, mix, and stir at 30°C Adsorbed for 1 hour, then filtered, and vacuum-dried to a moisture content of 0.5% to obtain lipase T1 immobilized on a macroporous adsorption resin, which was stored in a refrigerator at 4°C for later use. 9Kg refined soybean oil and 1.84Kg glycerol are added in raw material tank 1, and the moisture content in the control reaction system is no more than 0.1%, under the situation of magnetic stirring, as figure 1 As shown, the reactant is transported by the pump 4 and flows through the enzyme reactor 5 and then returns to the raw material tank 1. The amount of immobilized lipase LipaseT1 is 300 g, the reaction temperature is 80 ° C, and the entire reaction system is placed in a vacuum state with a pressure of 50 Pa. After 6 hours, the reaction was stopped, and the reaction product w...

Embodiment 3

[0028] Take 5Kg of activated macroporous weakly polar adsorption resin AB-8, 10Kg of Tris-HCl buffer (concentration 0.05mol / L, pH8.8), 100g of free lipase Lipase T1, mix and stir at 25°C Adsorbed for 1 hour, then filtered, and vacuum-dried to a moisture content of 0.6% to obtain lipase T1 immobilized on a macroporous adsorption resin, which was stored in a 4°C refrigerator for later use. 90Kg refined soybean oil and 18.4Kg glycerin are added in raw material tank 1, and the moisture content in the control reaction system is no more than 0.1%, under the situation of magnetic stirring, as figure 1 As shown, the reactants are pumped and flowed through the enzyme reactor and then returned to the raw material tank 1. The amount of immobilized lipase LipaseT1 is 3Kg, the reaction temperature is 80°C, and the entire reaction system is placed under a vacuum state of 50Pa. After 6 hours, the reaction was stopped, and the reaction product was centrifuged to get the upper oil phase for co...

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Abstract

The invention discloses a method of glycerolysis reaction for preparing partial glyceride, and belongs to the technical field of oil modification. The method comprises a first step of immobilization of Lipase T1, a second step of glycerolysis reaction of immobilized lipase catalysis oil, and a third step of separating products. The Lipase T1 is high in thermostability, after being immobilized through hydrophobicity macroreticular resin, the Lipase T1 can catalyze glycerolysis to be reacted under no-water environment in range of 75-95 DEG C. No by-product is produced, content of the partial glyceride is high in the products, immobilized enzyme can be recycled, and therefore the method of the glycerolysis reaction for preparing the partial glyceride has good application prospect in industries.

Description

technical field [0001] The invention relates to a method for producing oil rich in partial glycerides. Background technique [0002] Partial glyceride is a general term for monoglyceride and diglyceride, and both diglyceride and monoglyceride are very important food additives. As an important emulsifier, monoglyceride is widely used in food, pharmaceutical, cosmetics and other fields. Diacylglycerol functional edible oil is a new type of oil that is made from natural edible oil and catalyzed by biological enzymes. It has clear functions such as preventing obesity and lowering blood lipids, and is safe to eat. As a replacement product for ordinary edible oil, it is used in The premise of not changing the existing eating habits can fundamentally solve the excessive accumulation of lipids caused by excessive intake of fats. The production of partial glycerides can be divided into chemical method and enzymatic method according to different catalysts. The chemical method has t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N11/08
Inventor 王永华王卫飞杨博蓝东明李响宁柠
Owner YHSN TECH LTD
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