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Preparation method of diglyceride-enriched functional oil

A diglyceride, functional technology, applied in the field of preparation of functional oil rich in diglyceride, can solve the problems of many by-products, organic solvent residue, unfavorable food system application, etc., to achieve good catalytic effect and product The effect of cost reduction and good industrial application prospects

Inactive Publication Date: 2011-09-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This reaction produces many by-products, and there may be residual organic solvents, which is not conducive to the application in food systems

Method used

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  • Preparation method of diglyceride-enriched functional oil
  • Preparation method of diglyceride-enriched functional oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Weigh 5 g of activated DA-201 macroporous adsorption resin, 10 g of Tris-HCl buffer, 1 g of free phospholipase Lecitase ? Ultra was mixed, stirred and adsorbed at 25°C for 2 hours, and then filtered to obtain a macroporous adsorption resin immobilized phospholipase with an enzyme activity of 1850 U / g. Vacuum dry and set aside.

[0031] Weigh 10g of refined soybean oil in a flask, place it in a constant temperature magnetic stirrer, add 100g of glycerin, 10g of water and 5g of immobilized phospholipase Lecitase ? Ultra, set the reaction temperature at 40°C, and react at a stirring speed of 200r / min for 24 hours. After the reaction, the reaction mixture was poured into a centrifuge tube, and centrifuged at 8000×g for 20 min. The upper oil layer is the functional oil rich in diglycerides, the middle layer is the immobilized phospholipase Lecitase ? Ultra particles, the lower layer is unreacted glycerin. Taking the upper oil layer and measuring by high performance liquid c...

Embodiment 2

[0033] Weigh 5 g of activated DA-201 macroporous adsorption resin, 20 g of Tris-HCl buffer, 1 g of free phospholipase Lecitase ? Ultra was mixed, stirred and adsorbed at 30°C for 4 hours, and then filtered to obtain a macroporous adsorption resin immobilized phospholipase with an enzyme activity of 1462 U / g. Vacuum dry and set aside.

[0034] Weigh 10g refined palm oil in a flask, place it in a thermostatic magnetic stirrer, add 80g glycerin, 5g water and 6g immobilized phospholipase Lecitase ? Ultra, set the reaction temperature at 45°C, and react at a stirring speed of 300r / min for 36 hours. After the reaction, the reaction mixture was poured into a centrifuge tube and centrifuged at 10,000×g for 15 min. The upper oil layer is the functional oil rich in diglycerides. The content of diglycerides is 51.7 wt% as measured by high performance liquid chromatography. After the phospholipase immobilized on the macroporous adsorption resin is repeatedly used for 10 times according to...

Embodiment 3

[0036] Weigh 5 g of activated DA-201 macroporous adsorption resin, 20 g of Tris-HCl buffer, 1 g of free phospholipase Lecitase ? Ultra was mixed, stirred and adsorbed at 35°C for 3 hours, and then filtered to obtain a macroporous adsorption resin immobilized phospholipase with an enzyme activity of 1344 U / g. Vacuum dry and set aside.

[0037] Weigh 10g refined peanut oil in a flask, place it in a constant temperature magnetic stirrer, add 50g glycerin, 8g water and 8g immobilized phospholipase Lecitase ? Ultra, set the reaction temperature at 35°C, and react at a stirring speed of 250r / min for 24 hours. After the reaction, the reaction mixture was poured into a centrifuge tube and centrifuged at 6000×g for 30 min. The upper oil layer is a functional oil rich in diglycerides. The content of diglycerides is 52.5 wt% as measured by high performance liquid chromatography. After the phospholipase immobilized on the macroporous adsorption resin is repeatedly used for 12 times accord...

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Abstract

The invention relates to a preparation method of diglyceride-enriched functional oil. The method comprises following steps of: (1) immobilizing Lecitase Ultra; (2), carrying out a glycerolysis reaction on vegetable oil under the catalysis of immobilized Lecitase Ultra; and (3) carrying out product separation. The Lecitase Ultra used in the preparation method disclosed by the invention has low price which is only 5-10 percent of the price of lipase; and resin DA-201 for Lecitase Ultra immobilization has low cost. The immobilized Lecitase Ultra has good catalysis effect, high diglyceride content, less byproducts and recycling performance of the immobilized Lecitase Ultra; and therefore the diglyceride-enriched functional oil prepared by the preparation method disclosed by the invention has lower cost and favorable application prospect in the industry.

Description

Technical field [0001] The invention relates to the enzymatic preparation of functional oils rich in diglycerides, in particular to a method for preparing functional oils rich in diglycerides by glycerolysis of vegetable oils. Background technique [0002] Diacylglycerol (DAG) is a structural lipid in which a fatty acid in a class of triacylglycerol (TAG) is replaced by a hydroxyl group. It is a trace component of natural vegetable oils and an endogenous intermediate product of fat metabolism in the body, and is a food ingredient recognized as safe (GRAS). In recent years, many medical studies have shown that diglycerides have the functions of regulating blood lipids, preventing arterial thrombosis, inhibiting liver fat accumulation, relieving diabetic nephropathy, losing weight and suppressing appetite. In 2001, ADM, the largest grain and oil processing company in the United States, and KAO (Kao) of Japan established a joint venture company ADM Kao LLC. In 2003, Enova Oil, a he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N11/02
CPCY02P20/50
Inventor 赵谋明崔春刘宁汪勇
Owner SOUTH CHINA UNIV OF TECH
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