Supercharge Your Innovation With Domain-Expert AI Agents!

Fermentation production culture medium and culture method for aspergillus niger capable of selective synthesis of 1,3-diglyceride

A technology for the production of culture medium and diglyceride, which is applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of low catalytic efficiency of whole-cell lipase, and achieves favorable conditions for industrial production, mild conditions, and high efficiency. Easy-to-control effects

Inactive Publication Date: 2014-01-01
GUIZHOU UNIV
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since it is an intracellular lipase, the whole-cell lipase is directly used as a catalyst, but the catalytic efficiency of this whole-cell lipase is not high (about 11%)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fermentation production culture medium and culture method for aspergillus niger capable of selective synthesis of 1,3-diglyceride
  • Fermentation production culture medium and culture method for aspergillus niger capable of selective synthesis of 1,3-diglyceride
  • Fermentation production culture medium and culture method for aspergillus niger capable of selective synthesis of 1,3-diglyceride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The following are the culture medium and method involved in the present invention

[0024] (1) Seed medium: Seed medium (mass percentage): 0.3 beef extract; 0.5 peptone; 0.5 glucose; Bacteria for 20 min.

[0025] (2) Preparation of fermented seed liquid: Inoculate Aspergillus niger into the seed medium, culture at 30°C, 180r / min for 24 hours, and obtain the fermented seed liquid.

[0026] (3) Fermentation production medium (mass percentage): bean cake powder 2; corn steep liquor 2; K 2 HPO 4 0.5;NaNO 3 0.5; the rest is water, pH 7.0, in a 250mL Erlenmeyer flask, filled with 20%, and sterilized at 121°C for 20 minutes.

[0027] (4) Preparation of whole-cell lipase: Inoculate the fermented seed solution at 2% inoculum in the fermentation production medium for 72 hours at 30°C and 180r / min, then centrifuge at 4°C and 10,000r / min for 10min The obtained bacterium was freeze-dried and ground with liquid nitrogen protection to obtain whole-cell lipase.

[0028] (5) Gly...

Embodiment 2

[0031] Box-Behnken design

[0032] Box-Behnken design is an experimental method suitable for optimization of 2-5 factors. Several factors screened by PB experiments that significantly affect the synthesis of whole-cell lipase of Aspergillus niger CCTCC No. M2012538 and the yield of 1,3-diglyceride were used as design factors, and the concentration obtained from the steepest climbing test was used as the center With the yield of 1,3-diglyceride as the response value, the composition of the medium was optimized through response surface analysis. See Table 1 for the experimental design, Table 2 for the experimental results, and Table 3 for the analysis of the experimental results.

[0033]

[0034] Adopt Design-Expert software to carry out analysis of variance and multiple regression fitting to the data of 1,3-diglyceride yield in Table 2, obtain the significance of each influence factor (Table 3), obtain Aspergillus niger CCTCC No. M2012538 The quadratic polynomial regressi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a fermentation production culture medium for aspergillus niger capable of selective synthesis of 1,3-diglyceride. The medium is characterized by comprising the following components, by mass, 1.030% of glucose, 1.785% of soybean cake powder, 0.0441% of potassium chloride, 0.044% of disodium hydrogen phosphate, 0.044% of calcium chloride, 0.044% of dipotassium hydrogen phosphate and 0.044% of zinc sulfate, with the balance being water. The medium provided by the invention enables lipase to have greatly improved capability in catalysis of a glycerolysis reaction of glycerin and triglyceride and allows maximum yield of 1,3-diglyceride to reach 26.887%, increased by 2.7 times compared with yield before optimization. The proportion of 1,3-diglyceride in diglyceride is increased by 88.253%, increased by 1.26 times compared with the proportion before optimization.

Description

technical field [0001] The invention relates to a fermentation production medium and method of Aspergillus niger CCTCC NO.2012538 for highly selectively synthesizing 1,3-diglyceride. Background technique [0002] 1,3-Diglycerides are natural components of oils and are ideal for healthy fats. There are two reasons: one is that the metabolism of 1,3-diglyceride in the human body is different from that of 1,2-diglyceride and triglyceride. Eating 1,3-diglyceride can reduce blood lipids, reduce visceral fat and effectively It can prevent obesity and various diseases mainly caused by obesity; the second is that 1,3-diglyceride is no different from triglyceride in terms of taste and flavor. Studies have shown that in recent years, 1,3-diglyceride has been widely used in food processing and as a pharmaceutical intermediate, and its research has received extensive attention. [0003] Although 1,3-diglyceride has many advantages, its content in natural oils is very low, so it is par...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/685
Inventor 何腊平周换景李翠芹张义明
Owner GUIZHOU UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com