Method for preparing diglyceride through continuous glycerolysis by enzyme method

A diglyceride and glycerol hydrolysis technology, which is applied in the field of enzymatic continuous preparation of diglyceride, can solve the problems of large solvent usage, reduced enzyme activity, and difficulty in separation, so as to improve catalytic effect, prolong service life, and improve interaction solubility effect

Active Publication Date: 2009-04-01
无锡中粮工程科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the miscibility of glycerol and triglycerides is poor, and free glycerol can easily wrap enzymes and reduce enzyme activity
Therefore, under normal circumstances, it is difficult for lipase to catalyze the glycerolysis reaction
Many scholars have also improved this method, for example: ① U.S. Patent US6337414B1 provides a method for adding water to the reaction system to successfully realize the enzymatic glycerolysis reaction, because water is added in the method, so hydrolysis also occurs reaction, but a large amount of free fatty acids and monoglycerides are produced in the product, which increases the cost of separation of by-products
②Chinese patent CN200310112327.X provides a method of using oil and adsorbed glycerol as raw materials to prepare diglycerides through immobilized lipase catalyzed glycerol hydrolysis reaction. Adverse effects on enzymes, however, the adsorbent materials used are difficult to separate from enzymes and products, affecting the recycling of enzymes and the quality

Method used

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  • Method for preparing diglyceride through continuous glycerolysis by enzyme method
  • Method for preparing diglyceride through continuous glycerolysis by enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Put 100g of soybean oil and 20g of glycerin into the conical flask, then add 15mL of n-hexane, connect the condensing device, carry out magnetic stirring, and mix evenly, as figure 1 As shown, the reactant passed through two packed column reactors (10 cm in length, 1.5 cm in inner diameter, 3 cm in outer diameter, 55° C., with a condenser on top of the column) equipped with Novozyme 435 immobilized lipase at a flow rate of 0.5 mL / min. During the reaction process, the reactants (soybean oil, glycerin and n-hexane) were replenished in good time so as to conform to the ratio of raw materials and solvents in Table 1. Samples of different reaction times were collected respectively, and the organic solvent was removed by vacuum distillation (0.1Mpa, 70°C). , to obtain the product, analyzed by HPLC, the results are shown in Table 2.

Embodiment 2

[0025] 100g rapeseed oil and 15g glycerol were added into the Erlenmeyer flask and mixed evenly, then 18mL No. 6 solvent was added, and magnetic stirring was carried out, and the reactant passed through two packed column reactors equipped with Novozyme 435 immobilized lipase at a flow rate of 0.5mL / min ( The length is 10cm, the inner diameter is 1.5cm, the outer diameter is 3cm, 60°C, the top of the column is equipped with a condenser). During the reaction, the reactants (rapeseed oil, glycerol and No. 6 solvent) were replenished in time to meet the ratio of raw materials and solvents in Table 1. All the other operations are the same as in Example 1. The results are shown in Table 2.

Embodiment 3

[0027] 100g of sunflower oil and 10g of glycerol were added into the Erlenmeyer flask and mixed evenly, then 15mL of pentane was added, and magnetically stirred, and the reactant passed through two packed column reactors (length 10cm) equipped with Novozyme 435 immobilized lipase at a flow rate of 1mL / min. , inner diameter 1.5cm, outer diameter 3cm, 50°C, column top with condenser). During the reaction, the reactants (sunflower oil, glycerol and pentane) were replenished in good time to meet the ratio of raw materials and solvents in Table 1. All the other operations are the same as in Example 1. The results are shown in Table 2.

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Abstract

The invention relates to a method for preparing diglyceride through continuous enzymatic glycerolysis, which pertains to the technical field of gease modification. In the invention, glycerin, raw oil material and low-boiling single-phase organic solvent are fully mixed, and then continuous glycerolysis is carried out through a fixed bed reactor filled with immobilized lipase, and the products are distilled for removing the organic solvent to obtain the high-content diglyceride mixture. The invention greatly reduces the viscosity of the reaction system, improves the intersolubility of reaction materials, and protects the enzyme, thus improving the reaction efficiency and prolonging the service life of the enzyme.

Description

technical field [0001] The invention relates to a method for continuously preparing diglyceride by enzymatic method, which belongs to the technical field of oil modification. Background technique [0002] Diacylglycerol (DAG) is a natural component of oil and an intermediate product of oil metabolism. It is composed of one molecule of glycerol and two molecules of fatty acid, and has two isomers 1,3-DAG and 1,2-DAG, and the ratio of the two isomers in nature is 7:3. It is a food ingredient generally recognized as safe (GRAS) worldwide. Due to the different absorption and metabolism patterns of diacylglycerol and triacylglycerol, diacylglycerol has the functions of reducing visceral fat, inhibiting weight gain, and reducing blood lipids, so it has received extensive attention. Diacylglycerol has the flavor of ordinary oil and can replace edible oil in any proportion. Related products have been put on the market in Japan and the United States, and are deeply loved by consume...

Claims

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Application Information

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IPC IPC(8): C12P7/62
Inventor 姚专温琦华娣裘爱泳蒋蕴珍秦卫国陈志华朱小兵
Owner 无锡中粮工程科技有限公司
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