Creatine kinase isozyme bi-reagent and preparation method thereof

A creatine kinase and isoenzyme technology, which is applied in the field of immunoassay, can solve the problems that reagents are difficult to reach, and achieve the effects of small measurement value deviation, improved transportation stability, and improved shelf life

Active Publication Date: 2018-01-30
WUHAN LIFE ORIGIN BIOTECH LTD
View PDF9 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing reagents are di

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Creatine kinase isozyme bi-reagent and preparation method thereof
  • Creatine kinase isozyme bi-reagent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0111] In the creatine kinase isoenzyme double reagent of the embodiment of the present invention, the composition of reagent I is:

[0112] Addition amount of polyvinylpyrrolidone K12: mass fraction 0.01%

[0113] Addition amount of lauryl dihydroxyethylamine oxide: mass fraction 0.01%

[0114] Addition of tris(nonylphenyl)phosphite: mass fraction 0.05%

[0115] Antioxidant isomeric sodium ascorbate: mass concentration 0.05%

[0116] Imidazole buffer pH=6.0, 100mmol / L

[0117] N-acetylcysteine ​​20mmol / L

[0118] Phosphocreatine 30mmol / L

[0119] Adenosine diphosphate 5mmol / L

[0120] Adenosine triphosphate 5mmol / L

[0121] Glucose-6-phosphate dehydrogenase 1.5KU / L

[0122] Hexokinase 2.5KU / L

[0123] Creatine kinase isoenzyme antibody 2.0KU / L

[0124] the rest is water;

[0125] The composition of detection reagent II is

[0126] Nicotinamide adenine dinucleotide phosphate 2mmol / L

[0127] Glucose 20mmol / L;

[0128] The rest is water.

Embodiment 2

[0130] In the creatine kinase isoenzyme double reagent of the embodiment of the present invention, the composition of reagent I is:

[0131] Addition amount of polyvinylpyrrolidone K12: mass fraction 0.05%

[0132] Lauryl dihydroxyethylamine oxide addition amount: mass fraction 0.02%

[0133] Addition of tris(nonylphenyl)phosphite: mass fraction 0.01%

[0134] Antioxidant isomeric sodium ascorbate: mass concentration 0.05%

[0135] Imidazole buffer pH=6.0, 100mmol / L

[0136] N-acetylcysteine ​​20mmol / L

[0137] Phosphocreatine 30mmol / L

[0138] Adenosine diphosphate 5mmol / L

[0139] Adenosine triphosphate 5mmol / L

[0140] Glucose-6-phosphate dehydrogenase 1.5KU / L

[0141] Hexokinase 2.5KU / L

[0142] Creatine kinase isoenzyme antibody 2.0KU / L;

[0143] the rest is water;

[0144] The composition of detection reagent II is:

[0145] Nicotinamide adenine dinucleotide phosphate 2mmol / L

[0146] Glucose 20mmol / L,

[0147] The rest is water.

Embodiment 3

[0149] In the creatine kinase isoenzyme double reagent of the embodiment of the present invention, the composition of reagent I is:

[0150] Addition amount of polyvinylpyrrolidone K12: mass fraction 0.01%

[0151] Addition amount of lauryl dihydroxyethylamine oxide: mass fraction 0.01%

[0152] Addition of tris(nonylphenyl)phosphite: mass fraction 0.05%

[0153] Antioxidant isomeric sodium ascorbate: mass concentration 0.05%

[0154] Imidazole buffer pH=6.0, 120mmol / L

[0155] N-acetylcysteine ​​25mmol / L

[0156] Phosphocreatine 35mmol / L

[0157] Adenosine diphosphate 10mmol / L

[0158] Adenosine triphosphate 10mmol / L

[0159] Glucose-6-phosphate dehydrogenase 1.5KU / L

[0160] Hexokinase 2.5KU / L

[0161] Creatine kinase isoenzyme antibody 2.0KU / L

[0162] the rest is water;

[0163] The composition of detection reagent II is:

[0164] Nicotinamide adenine dinucleotide phosphate 5mmol / L

[0165] Glucose 30mmol / L;

[0166] The rest is water.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention mainly aims at providing a creatine kinase isozyme bi-reagent and a preparation method thereof. The reagent I is prepared from the following components: 90 to 120mmol/L of imidazole buffer solution, 15 to 25mmol/L of N-acetylcysteine, 25 to 35mmol/L of phosphocreatine, 1 to 10mmol/L of adenosine diphosphate, 1 to 10mmol/L of triphosadenine, greater than 1.5KU/L of glucose-6-phosphatedehydrogenase, greater than 2.5KU/L of heterophosphatase and greater than 2.0KU/L of creatine kinase isozyme antibody; 0.01 to 0.05 percent by mass concentration of polyvinylpyrrolidone, 0.01 to 0.05percent by mass concentration of tris(nonylphenyl) phosphate, 0.01 to 0.02 percent by mass concentration of lauryl dihydroxyethyl amine oxide, 0.05 percent of isomeric sodium ascorbate and the balance of water; the reagent II is prepared from the following components: by adopting a reagent II solution as a basis, 1 to 5mmol/L of nicotinamide adenine dinucleotide phosphate, 10 to 30mmol/L of glucose and the balance of water. In the case of storing for 14 days at 37 DEG C, the reagent stability is still relatively high, and measurement value deviation of the reagent is less than 10 percent.

Description

technical field [0001] The invention relates to the field of immunoassay, in particular to a creatine kinase isoenzyme double reagent and a preparation method thereof. Background technique [0002] Creatine kinase isoenzyme assay kit (selective inhibition method) is used to determine the content of creatine kinase isoenzyme in human serum or plasma. The most important significance of serum CK-MB (creatine kinase isoenzyme) determination lies in the diagnosis of acute myocardial infarction. Clinically, CK-MB exceeds the total activity of CK as the basis for the diagnosis of acute myocardial infarction. Creatine kinase is a kinase that plays a vital role in energy transport and metabolism in cells. Creatine kinase isoenzyme is an isoenzyme of creatine kinase, which is produced by post-translational modification and mainly exists in cardiomyocytes (and a small amount exists in skeletal muscle, intestinal tract, tongue, prostate and In the uterus), is a very important creatin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/48C12Q1/32
Inventor 华权高龚婷徐春雷
Owner WUHAN LIFE ORIGIN BIOTECH LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap