Creatine kinase isozyme bi-reagent and preparation method thereof

Abstract

The invention mainly aims at providing a creatine kinase isozyme bi-reagent and a preparation method thereof. The reagent I is prepared from the following components: 90 to 120mmol / L of imidazole buffer solution, 15 to 25mmol / L of N-acetylcysteine, 25 to 35mmol / L of phosphocreatine, 1 to 10mmol / L of adenosine diphosphate, 1 to 10mmol / L of triphosadenine, greater than 1.5KU / L of glucose-6-phosphatedehydrogenase, greater than 2.5KU / L of heterophosphatase and greater than 2.0KU / L of creatine kinase isozyme antibody; 0.01 to 0.05 percent by mass concentration of polyvinylpyrrolidone, 0.01 to 0.05percent by mass concentration of tris(nonylphenyl) phosphate, 0.01 to 0.02 percent by mass concentration of lauryl dihydroxyethyl amine oxide, 0.05 percent of isomeric sodium ascorbate and the balance of water; the reagent II is prepared from the following components: by adopting a reagent II solution as a basis, 1 to 5mmol / L of nicotinamide adenine dinucleotide phosphate, 10 to 30mmol / L of glucose and the balance of water. In the case of storing for 14 days at 37 DEG C, the reagent stability is still relatively high, and measurement value deviation of the reagent is less than 10 percent.

Description

technical field [0001] The invention relates to the field of immunoassay, in particular to a creatine kinase isoenzyme double reagent and a preparation method thereof. Background technique [0002] Creatine kinase isoenzyme assay kit (selective inhibition method) is used to determine the content of creatine kinase isoenzyme in human serum or plasma. The most important significance of serum CK-MB (creatine kinase isoenzyme) determination lies in the diagnosis of acute myocardial infarction. Clinically, CK-MB exceeds the total activity of CK as the basis for the diagnosis of acute myocardial infarction. Creatine kinase is a kinase that plays a vital role in energy transport and metabolism in cells. Creatine kinase isoenzyme is an isoenzyme of creatine kinase, which is produced by post-translational modification and mainly exists in cardiomyocytes (and a small amount exists in skeletal muscle, intestinal tract, tongue, prostate and In the uterus), is a very important creatin...

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Problems solved by technology

However, the existing reagents are difficult to meet the above requirements

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