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Sodium diagnosis/measuring reagent kit and method for measuring sodium concentration

A kit and reagent technology, applied in the field of medical/food/environmental inspection and determination, can solve the problems of complicated operation, the inability of the biochemical analyzer to measure the content of sodium ions and potassium ions at the same time, and the inability to be popularized and applied.

Inactive Publication Date: 2008-05-07
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because there are holes in the macrocyclic structure, the oxygen atoms in the molecule have unshared electron pairs that can be combined with metal ions. According to the size of the holes, metal ions of different diameters can be selectively combined to achieve the purpose of measuring the ion concentration; Spectrophotometry can also be used to detect K in serum + 、Na + , but the operation is complicated, the error is large, and it is not as simple as the flame photometry
[0007] The method of enzymatic determination of sodium ion content has a good consistency with the flame photometer method or ion selective electrode method. This method has strong anti-interference and good stability, but the biochemical analyzer cannot simultaneously measure sodium ion and The respective content of potassium ions makes this method unable to be popularized and applied.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The sodium diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0050] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0051] Stabilizer 50mmol / L

[0052] Coenzyme 3mmol / L

[0053] Lactose 20mmol / L

[0054] Adenosine triphosphate 6mmol / L

[0055] Magnesium chloride 6mmol / L

[0056] β-Galactosidase 10000U / L

[0057] Hexokinase 10000U / L

[0058] Glucose-6-phosphate dehydrogenase 10000U / L

[0059] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0060] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured sodium sample to the reagent is 1 / 25, the reaction The direction is positive reaction (rising reaction), the delay time i...

Embodiment 2

[0063] The sodium diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0064] Reagent 1

[0065] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0066] Stabilizer 50mmol / L

[0067] Coenzyme 3mmol / L

[0068] Lactose 10mmol / L

[0069] Adenosine triphosphate 9mmol / L

[0070] Magnesium chloride 3mmol / L

[0071] Reagent 2

[0072] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0073] Stabilizer 50mmol / L

[0074] β-Galactosidase 16000U / L

[0075] Hexokinase 16000U / L

[0076] Glucose-6-phosphate dehydrogenase 16000U / L

[0077] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0078] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured sodium sample to...

Embodiment 3

[0081] The sodium diagnosis / determination reagent of the present embodiment is three reagents, comprising:

[0082] Reagent 1

[0083] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0084] Stabilizer 50mmol / L

[0085] Coenzyme 3mmol / L

[0086] Adenosine triphosphate 12mmol / L

[0087] Reagent 2

[0088] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0089] Lactose 30mmol / L

[0090] Magnesium chloride 4mmol / L

[0091] Reagent 3

[0092] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0093] Stabilizer 50mmol / L

[0094] β-Galactosidase 20000U / L

[0095] Hexokinase 8000U / L

[0096] Glucose-6-phosphate dehydrogenase 8000U / L

[0097] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0098] When measuring the sodium concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, ...

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PUM

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Abstract

The invention relates to a natrium diagnosis / determination reagent kit which utilizing a enzyme colorimetry method and an enzyme-linked method technology, simultaneously the invention also relates to a method principle for determining the natrium concentration, the composition and the components of a reagent, belonging to the technical field of the medicine / food / environment examination and determination. The main components of the reagent kit of the invention comprise buffer solution, coenzyme, deoxycholate citrate lactose sacharose agar, adenosine triphosphate, magnesium chloride, Beta-galactosidase, hexokinase, dextrose-6-phosphate dehydrogenase and stabilizer. A sample is mixed with the reagent according to a certain volume to lead to perform the enzymatic reaction, a reacting substance is put under an ultraviolet / visible light analyzer to examine the rising speed of absorbance at the position of a 340nm dominant wavelength, thereby detecting and calculating the natrium concentration value. Through the adoption of the invention, the required determined results can be obtained completely through the ultraviolet / visible light analyzer.

Description

technical field [0001] The invention relates to a sodium diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring sodium concentration, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique [0002] The content of sodium ions in serum (referred to as "blood sodium") has very important clinical significance. There are three decision levels in medicine, in order: level 1—115mmol / l, level 2—135mmol / l, level 3— -150mmol / l. When the patient's blood sodium concentration is equal to or lower than level 1, symptoms such as confusion, fatigue, nausea, headache, vomiting, and anorexia may appear, indicating that the proportion of water in the body is greater than sodium, and corresponding treatment measures should be taken; when the blood sodium concentration When it is lower than level 2, the cause of hyponatremia should be found, and the serum osmotic pressure, blood potass...

Claims

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Application Information

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IPC IPC(8): G01N33/84G01N21/78G01N21/25C12Q1/32C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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