Kit for detecting glucose by hexokinase method and preparation method

A glucose and kit technology, which is applied in the field of kits and preparations for glucose detection by hexokinase method, can solve problems such as endogenous interference and the like

Inactive Publication Date: 2009-03-18
TIANJIN BAODI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0051] In order to solve the problem of endogenous interference in the determination of glucose by the hexokinase method in the prior art, the present invention provides a hexokinase that ...

Method used

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  • Kit for detecting glucose by hexokinase method and preparation method
  • Kit for detecting glucose by hexokinase method and preparation method
  • Kit for detecting glucose by hexokinase method and preparation method

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Experimental program
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Embodiment Construction

[0122] 1. Composition of the kit

[0123] 4 bottles of reagent I (100ml / bottle); 1 bottle of reagent II (100ml); 1 glucose standard solution (concentration: 5.55mmol / L, 2ml); 1 kit instruction manual.

[0124] 2. Preparation of reagents:

[0125] Preparation of triethanolamine hydrochloride buffer solution (concentration 50mmol / L):

[0126] Dissolve 14.92g of triethanolamine in 2L of distilled water, then add 40mg of ProClin 300 into the solution and mix well. At 25°C, use 1mol / L hydrochloric acid to adjust the pH of the triethanolamine hydrochloride buffer to 7.5±0.2.

[0127] The preparation of reagent I:

[0128] Dissolve 10.0 g of HK in 1 L of prepared triethanolamine hydrochloride buffer solution, add 1.07 g of sodium oxalate, 2.22 g of ATP, and 226.28 mg of anhydrous MgSO to the above-mentioned triethanolamine hydrochloride buffer mixture, and mix well to prepare reagent I.

[0129] b. Preparation of Reagent II:

[0130] 6.63mgNAD + Dissolve 3.60 mg of G6PD in anoth...

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Abstract

The invention discloses a reagent kit for detecting glucose by a hexokinase method, which belongs to a reagent kit in a method for detection glucose containing enzyme. The reagent kit comprises four bottles of reagent I, one bottle of reagent II, and one glucose standard liquid, wherein the reagent I comprises oxalate with the concentration of 8.0mmol/L. By inhibiting the activities of endogenous lactate dehydrogenase and alpha-hydroxybutyrate dehydrogenase through the oxalate, the reagent kit controls an interference reaction, increases the specificity of the reaction, and improves the accuracy of detection results. The reagent kit is slightly affected by the volume ratio of sample reagents and the reaction time during the detection, and the linear range can reach 37.4mmol/L. A use method and the range of the reagent kit are the same as those of the prior hexokinase method; and the reagent kit can not increase the burden of experimental personnel, almost does not increase the cost of reagents, is economical, convenient and easy, and is the reagent kit which detects the glucose by the hexokinase method with higher accuracy.

Description

technical field [0001] The invention belongs to a kit for a glucose detection method containing an enzyme, in particular to a kit for detecting glucose by a hexokinase method and a preparation method. Background technique [0002] There are as many as 12 known methods for the determination of glucose. Among these methods, some methods are susceptible to interference from reducing substances and other sugars in the reaction solution, resulting in false negative and false positive deviations and are rarely used. At present, the most commonly used method for detecting glucose at home and abroad is the enzymatic method. Using enzymes as reagents can improve specificity. Glucose oxidase (GOD) method and hexokinase (HK) method are often used to measure glucose in clinical laboratories. The glucose oxidase method is easily interfered by vitamin C, uric acid, urea, bilirubin, and creatinine, but the above substances have no interference with the hexokinase method. The hexokinase me...

Claims

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Application Information

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IPC IPC(8): C12Q1/54C12Q1/32
Inventor 李立和王高生
Owner TIANJIN BAODI HOSPITAL
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