Separation and purification method for Amanita virosa antibacterial active ingredient

Abstract

The invention relates to a separation and purification method of a Basidiomycetes antifungal component; the antifungal component is composed of two amide-type substances, and the molecular weight of the two substances is 5732.39 and 7187.01; the separation and purification process comprises the following steps: Basidiomycetes entity authentication, strain separation and purification, strain identification, mycelium liquid culture, antifungal component extraction, antifungal component separation, antifungal component purification. The method can inhibit chrysosperma mycelium growth and spore germination with 100 percent of inhibition rate, and has obvious inhibiting function to Pinus sylvestris germ at the same time. The Basidiomycetes antifungal substance affects the activity of seven enzymes of pathogenicbacteria, reduces the activity of hexokinase, improves the activity of pyruvate kinase, succinic acid, lactate dehydrogenase and ATP enzyme, and speeds up the respiration of germs.

Description

technical field

[0001] The invention relates to a method for separating and purifying antibacterial active components of Amanita chinensis. Background technique

[0002] At present, Amanita virosa belongs to Basidiomycotina, Hymenomycetes, Agaricales, Amanitaceae, and Amanita. fungus. The fungus is extremely toxic. At present, there is no report on the use of antibacterial active ingredients in the fermentation broth of Amanita chinensis mycelia—amides to control forest diseases. Contents of the invention

[0003] The object of the present invention is to provide a method of using the antibacterial active ingredients in the Amanita chinensis mycelium fermentation liquid to prevent and control forest diseases.

[0004] In order to achieve the above object, the technical scheme adopted in the present invention is: antibacterial active ingredient is made of two kinds of amide substances, and molecular weight is respectively 5732.39,7187.01, and separation and purification ...

Examples

Embodiment 1

[0016] (1) Carry out fruit body identification according to the characteristics of the fruiting body of Amanita chinensis. The fruiting body of Amanita chinensis is solitary to scattered, with a cap diameter of 6-15cm, conical to bell-shaped, flat at the back, and convex in the center , the surface is sticky when wet, shiny when dry, white, sometimes slightly yellowish in the center, smooth. Gills white, free, dense, stipe 8-14cm long, 1-1.2cm in diameter, white, with prominent scales, nearly columnar, base swollen spherical, fungus ring born on the upper part of the stipe, white, membranous , drooping, not easy to fall off, white receptacle, bract-shaped, white spore print, nearly spherical spores, colorless, smooth, 7-10μm,

[0017] (2), strain separation, purification: adopt tissue separation method, under aseptic condition, pick the tissue between stipe and cap to be inoculated on PDA plate culture medium, treat that mycelium grows on the tissue block, bacterial growth Si...

Embodiment 2

[0024] (1) Identify the fruiting body according to the characteristics of the fruiting body of Amanita chinensis. The fruiting body is solitary to scattered, the diameter of the cap is 6-15cm, conical to bell-shaped, flat at the back, convex in the center, and there are spots on the surface when wet Viscous, shiny when dry, white, sometimes slightly yellowish in the center, smooth. Gills white, free, dense, stipe 8-14cm long, 1-1.2cm in diameter, white, with prominent scales, nearly columnar, base swollen spherical, fungus ring born on the upper part of the stipe, white, membranous , drooping, not easy to fall off, white receptacle, bract-shaped, white spore print, nearly spherical spores, colorless, smooth, 7-10μm,

[0025] (2), strain separation, purification: adopt tissue separation method, under aseptic condition, pick the tissue between stipe and cap to be inoculated on PDA plate culture medium, treat that mycelium grows on the tissue block, bacterial growth Transfer sil...

Embodiment 3

[0032] (1) Identify the fruiting body according to the characteristics of the fruiting body of Amanita chinensis. The fruiting body is solitary to scattered, the diameter of the cap is 6-15cm, conical to bell-shaped, flat at the back, convex in the center, and there are spots on the surface when wet Viscous, shiny when dry, white, sometimes slightly yellow in the center, smooth, gills white, free, dense, stipe 8-14cm long, 1-1.2cm in diameter, white, with prominent scales, nearly columnar, The base is enlarged and spherical, and the ring is born on the upper part of the stipe, white, membranous, drooping, not easy to fall off, and the receptacle is white and bract-shaped. The spore print is white, the spores are nearly spherical, colorless, smooth, 7-10μm,

[0033] (2), strain separation, purification: adopt tissue separation method, under aseptic condition, pick the tissue between stipe and cap to be inoculated on PDA plate culture medium, treat that mycelium grows on the tis...

technical field

[0001] The invention relates to a method for separating and purifying antibacterial active components of Amanita chinensis. Background technique

[0002] At present, Amanita virosa belongs to Basidiomycotina, Hymenomycetes, Agaricales, Amanitaceae, and Amanita. fungus. The fungus is extremely toxic. At present, there is no report on the use of antibacterial active ingredients in the fermentation broth of Amanita chinensis mycelia—amides to control forest diseases. Contents of the invention

[0003] The object of the present invention is to provide a method of using the antibacterial active ingredients in the Amanita chinensis mycelium fermentation liquid to prevent and control forest diseases.

[0004] In order to achieve the above object, the technical scheme adopted in the present invention is: antibacterial active ingredient is made of two kinds of amide substances, and molecular weight is respectively 5732.39,7187.01, and separation and purification ...

Examples

Embodiment 1

[0016] (1) Carry out fruit body identification according to the characteristics of the fruiting body of Amanita chinensis. The fruiting body of Amanita chinensis is solitary to scattered, with a cap diameter of 6-15cm, conical to bell-shaped, flat at the back, and convex in the center , the surface is sticky when wet, shiny when dry, white, sometimes slightly yellowish in the center, smooth. Gills white, free, dense, stipe 8-14cm long, 1-1.2cm in diameter, white, with prominent scales, nearly columnar, base swollen spherical, fungus ring born on the upper part of the stipe, white, membranous , drooping, not easy to fall off, white receptacle, bract-shaped, white spore print, nearly spherical spores, colorless, smooth, 7-10μm,

[0017] (2), strain separation, purification: adopt tissue separation method, under aseptic condition, pick the tissue between stipe and cap to be inoculated on PDA plate culture medium, treat that mycelium grows on the tissue block, bacterial growth Si...

Embodiment 2

[0024] (1) Identify the fruiting body according to the characteristics of the fruiting body of Amanita chinensis. The fruiting body is solitary to scattered, the diameter of the cap is 6-15cm, conical to bell-shaped, flat at the back, convex in the center, and there are spots on the surface when wet Viscous, shiny when dry, white, sometimes slightly yellowish in the center, smooth. Gills white, free, dense, stipe 8-14cm long, 1-1.2cm in diameter, white, with prominent scales, nearly columnar, base swollen spherical, fungus ring born on the upper part of the stipe, white, membranous , drooping, not easy to fall off, white receptacle, bract-shaped, white spore print, nearly spherical spores, colorless, smooth, 7-10μm,

[0025] (2), strain separation, purification: adopt tissue separation method, under aseptic condition, pick the tissue between stipe and cap to be inoculated on PDA plate culture medium, treat that mycelium grows on the tissue block, bacterial growth Transfer sil...

Embodiment 3

[0032] (1) Identify the fruiting body according to the characteristics of the fruiting body of Amanita chinensis. The fruiting body is solitary to scattered, the diameter of the cap is 6-15cm, conical to bell-shaped, flat at the back, convex in the center, and there are spots on the surface when wet Viscous, shiny when dry, white, sometimes slightly yellow in the center, smooth, gills white, free, dense, stipe 8-14cm long, 1-1.2cm in diameter, white, with prominent scales, nearly columnar, The base is enlarged and spherical, and the ring is born on the upper part of the stipe, white, membranous, drooping, not easy to fall off, and the receptacle is white and bract-shaped. The spore print is white, the spores are nearly spherical, colorless, smooth, 7-10μm,

[0033] (2), strain separation, purification: adopt tissue separation method, under aseptic condition, pick the tissue between stipe and cap to be inoculated on PDA plate culture medium, treat that mycelium grows on the tis...