Ratio fluorescence analysis method for detecting mercury ions

An analytical method and a ratiometric fluorescence technique, applied in the field of fluorescence analysis, to achieve the effects of broad application prospects, high precision and sensitivity, and simple operation

Active Publication Date: 2019-07-16
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] There is no report on the ratiometric fluorescence analysis method based on silicon-doped carbon quantum dots and platinum-gold nanoparticles to detect mercury ions

Method used

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  • Ratio fluorescence analysis method for detecting mercury ions
  • Ratio fluorescence analysis method for detecting mercury ions
  • Ratio fluorescence analysis method for detecting mercury ions

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Embodiment 1

[0039] This embodiment provides a ratiometric fluorescence analysis method for detecting mercury ions, which specifically includes the following steps:

[0040] Step S11. Synthesis of platinum-gold nanoparticles: 0.9 mL K 2 PtCl 4 (20 mM), 0.1 mL HAuCl 4 (20mM) mixed, add 10 mg of poloxamer (Pluronic) F127, ultrasonic to dissolve it, then add 1.0 mL of ascorbic acid (100mM) as a reducing agent, the mixed solution was ultrasonically reacted in a water bath at 30ºC for 5 hours, and the product was subjected to 10000r / min Centrifuge for 20 min to separate, wash with ethanol and water repeatedly 5 times to remove residual PluronicF127, and reconstitute with 0.9 mL of water for later use.

[0041] It can be seen from Figure 1 that the prepared platinum nanoparticles are porous nanostructures with a particle size of about 50 nm, and the surface is composed of uniformly distributed Pt nanoparticles of about 5 nm.

[0042] Step S12. Synthesis of silicon-doped carbon quantum dots: t...

Embodiment 2

[0050] This embodiment provides a ratiometric fluorescence analysis method for detecting mercury ions, which specifically includes the following steps:

[0051] Step S21. Synthesis of platinum-gold nanoparticles: 0.7 mL K 2 PtCl 4 (20 mM), 0.1 mL HAuCl 4 (20mM) mixed, add 10 mg of poloxamer (Pluronic) F127, ultrasonic to dissolve it, then add 1.0 mL of ascorbic acid (100mM) as a reducing agent, the mixed solution was ultrasonically reacted in a water bath at 30ºC for 8 hours, and the product was subjected to 10000r / min Centrifuge for 20 min to separate, wash with ethanol and water repeatedly 5 times to remove residual PluronicF127, and reconstitute with 0.9 mL of water for later use.

[0052] Step S22. Synthesis of silicon-doped carbon quantum dots: Take 0.2M N-β-(aminoethyl)-γ-aminopropyltrimethoxysilane in a 100 mL three-necked flask, degas with nitrogen for 5 min , heated to 240°C, quickly added 0.05M anhydrous citric acid under vigorous stirring, and kept heating at thi...

Embodiment 3

[0056] The present embodiment provides a ratiometric fluorescence analysis method for detecting mercury ions, which specifically includes the following steps:

[0057]Step S31. Synthesis of platinum-gold nanoparticles: 0.5 mL K 2 PtCl 4 (20 mM), 0.1 mL HAuCl 4 (20mM) mixed, add 10 mg of poloxamer (Pluronic) F127, ultrasonic to dissolve it, then add 1.0 mL of ascorbic acid (100mM) as a reducing agent, the mixed solution was ultrasonically reacted in a water bath at 30ºC for 10h, and the product was subjected to 10000r / min Centrifuge for 20 min to separate, wash with ethanol and water repeatedly 5 times to remove residual PluronicF127, and reconstitute with 0.9 mL of water for later use.

[0058] Step S32. Synthesis of silicon-doped carbon quantum dots: take 0.15M N-β-(aminoethyl)-γ-aminopropyltrimethoxysilane in a 100 mL three-necked flask, degas with nitrogen for 5 min , heated to 240°C, quickly added 0.05M anhydrous citric acid under vigorous stirring, and kept heating at ...

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Abstract

The invention belongs to the field of fluorescence analysis technology, and specifically discloses a ratio fluorescence analysis method for detecting mercury ions based on silicon-doped carbon quantumdots and platinum-gold nano-particles. The ratio fluorescence analysis method for detecting the mercury ions specifically comprises that: the silicon-doped carbon quantum dot which can emit blue fluorescence and is prepared by using N-[Beta]-(aminoethyl)-[Gamma]-aminopropyl trimethoxysilane and citric acid as a silicon source and a carbon source separately is used as a reference fluorophore; andthe platinum-gold nano-particles catalyze the OPD to produce a DAP-responsive fluorophore which can emit orange-yellow fluorescence; meanwhile, since the DAP characteristic absorption spectrum mostlyoverlaps with the emission spectrum of the silicon-doped carbon quantum, the DAP can efficiently quench the silicon-doped carbon quantum dots. Meanwhile, the catalytic effect of the platinum-gold nano-particles can be specifically inhibited by using the metal-selective effect of the mercury ions; therefore, the detection of trace mercury ions using the double-emission ratio fluorescence analysis method is realized. The ratio fluorescence analysis method for detecting the mercury ions has the advantages such as simple operation, high sensitivity, quickness and convenience, and can be used for rapid detection of mercury ions, which has broad application prospects.

Description

technical field [0001] The invention belongs to the field of fluorescence analysis, in particular to a ratio fluorescence analysis method for detecting mercury ions based on silicon-doped carbon quantum dots and platinum-gold nanoparticles. Background technique [0002] Heavy metal mercury is a metal element with severe physiological toxicity, and it is also one of the most concerned environmental pollutants. Inorganic mercury ions in the environment can be converted by organisms into highly toxic methylmercury under certain conditions. Inorganic mercury mainly affects the kidneys, while methylmercury mainly damages the nervous system after entering the human body, especially the central nervous system. Both can be highly enriched in biological tissues through the food chain, thus causing great harm to humans and nature. Mercury poisoning will have an extremely bad impact on the whole society. Mercury is now prioritized on the list of the global environmental monitoring sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/31B82Y30/00
CPCB82Y30/00G01N21/314G01N21/3151G01N21/6428G01N21/6489
Inventor 罗林贾宝珠徐振林孙远明戚凯欣卢胜洪
Owner SOUTH CHINA AGRI UNIV
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