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Preparation method of ratio fluorescence aptamer sensor for detecting zearalenone based on photoinduced electron transfer

A zearalenone, aptamer sensor technology, applied in chemical instruments and methods, biochemical equipment and methods, fluorescence/phosphorescence, etc., to simplify the experimental process, improve stability, and avoid material loss.

Pending Publication Date: 2021-09-03
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, studies on PET-based ratiometric fluorescent aptasensors for the detection of ZEN assays have not been reported yet.

Method used

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  • Preparation method of ratio fluorescence aptamer sensor for detecting zearalenone based on photoinduced electron transfer
  • Preparation method of ratio fluorescence aptamer sensor for detecting zearalenone based on photoinduced electron transfer
  • Preparation method of ratio fluorescence aptamer sensor for detecting zearalenone based on photoinduced electron transfer

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Experimental program
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Embodiment 1

[0042] (1) Synthesize N,S-CQDs and make a solution for later use;

[0043] 4.5g of citric acid, 4.5g of urea and 1.125g of L-cysteine ​​were dissolved in 10mL of water to form a transparent mixed solution, which was then heated in a household microwave oven (700W) for 5min to obtain a dark brown solid. 10 mg of solid was redispersed in 20 mL of water and centrifuged (10000 rpm, 15 min). After filtering through a 0.22 μm filter membrane, store in the dark at 4°C for later use.

[0044] (2) Synthesis of CdTe QDs-apt, standby;

[0045] a. Preparation of precursor solution: mix 0.0646g Te and 0.0457g NaBH 4 Dissolve in 4mL of water, pass nitrogen gas for 20min, then stand at 4°C for 8h;

[0046] b. Preparation of CdTe QDs solution: under stirring conditions, 0.1142g CdCl 2 2.5H 2 O was dissolved in 50 mL of water, 75 μL of mercaptopropionic acid was added, and then the pH of the solution was adjusted to 8.5 with 1mol / L NaOH solution, and nitrogen gas was passed for 15 min; 2 ...

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Abstract

The invention belongs to the technical field of aptamer sensing detection, and relates to a preparation method of a ratio fluorescence aptamer sensor based on photoinduced electron transfer and application of the ratio fluorescence aptamer sensor in detecting ZEN. The preparation method specifically comprises the following steps: respectively taking fluorescence signals generated by N,S-CQDs and aptamer modified CdTe QDs-apt as a reference signal and a response signal, and obtaining the ratio fluorescence aptamer sensor based on photoinduced electron transfer by utilizing photoinduced electron transfer between WS2NTs and CdTe QDs-apt, a shearing effect of Exo I on CdTe QDs-apt and the characteristic of specific binding of an aptamer to ZEN, so that high-sensitivity, high-precision and high-selectivity detection of ZEN in an actual sample is realized. The linear response range of the sensor constructed by the invention to ZEN is 0.1-100 pg / mL, the detection limit is 0.1 pg / mL, and high-performance analysis of ZEN is successfully realized.

Description

technical field [0001] The invention belongs to the technical field of aptamer sensing and detection, and relates to a method for preparing a ratiometric fluorescent aptamer sensor based on the photoinduced electron transfer effect, which is used for sensitive and accurate detection of zearalenone (ZEN). detection. Background technique [0002] Zearalenone (ZEN) is a secondary toxic metabolite produced by Fusarium fungus infection, which widely exists in corn, wheat, barley, rice and other crops. Severe pollution of ZEN will not only cause serious economic losses in planting and animal husbandry, but also cause diseases such as teratogenicity, carcinogenicity, neurotoxicity and reproductive toxicity, which seriously threaten the health of animals and humans. In view of this, my country included ZEN in the current national food safety limit standard GB 2761-2017 "Mycotoxin Limits in Food" in 2017, and made clear instructions on the limit of ZEN, food types, etc., in order to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6825C09K11/88C09K11/02G01N21/64
CPCC12Q1/6825C09K11/883C09K11/025G01N21/643G01N2021/6432C12Q2525/205C12Q2563/107C12Q2521/319C12Q2565/607Y02A50/30
Inventor 由天艳毕晓雅李丽波李佳敏
Owner JIANGSU UNIV
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