Preparation method of ratio fluorescence aptamer sensor for detecting zearalenone based on photoinduced electron transfer
A zearalenone, aptamer sensor technology, applied in chemical instruments and methods, biochemical equipment and methods, fluorescence/phosphorescence, etc., to simplify the experimental process, improve stability, and avoid material loss.
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[0042] (1) Synthesize N,S-CQDs and make a solution for later use;
[0043] 4.5g of citric acid, 4.5g of urea and 1.125g of L-cysteine were dissolved in 10mL of water to form a transparent mixed solution, which was then heated in a household microwave oven (700W) for 5min to obtain a dark brown solid. 10 mg of solid was redispersed in 20 mL of water and centrifuged (10000 rpm, 15 min). After filtering through a 0.22 μm filter membrane, store in the dark at 4°C for later use.
[0044] (2) Synthesis of CdTe QDs-apt, standby;
[0045] a. Preparation of precursor solution: mix 0.0646g Te and 0.0457g NaBH 4 Dissolve in 4mL of water, pass nitrogen gas for 20min, then stand at 4°C for 8h;
[0046] b. Preparation of CdTe QDs solution: under stirring conditions, 0.1142g CdCl 2 2.5H 2 O was dissolved in 50 mL of water, 75 μL of mercaptopropionic acid was added, and then the pH of the solution was adjusted to 8.5 with 1mol / L NaOH solution, and nitrogen gas was passed for 15 min; 2 ...
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