A Ratiometric Fluorescence Analysis Method for Detecting Nitrite

An analytical method and a nitrite technology are applied in the field of fluorescence analysis to achieve the effects of simple synthesis, broad application prospects, and high precision and sensitivity

Active Publication Date: 2022-07-01
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] There is no report on the ratiometric fluorescence analysis method for the detection of nitrite based on graphene quantum dots and o-phenylenediamine derivatization

Method used

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  • A Ratiometric Fluorescence Analysis Method for Detecting Nitrite
  • A Ratiometric Fluorescence Analysis Method for Detecting Nitrite
  • A Ratiometric Fluorescence Analysis Method for Detecting Nitrite

Examples

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Embodiment 1

[0044] The present embodiment provides a ratiometric fluorescence analysis method for detecting nitrite, which specifically includes the following steps:

[0045] Step S11. Synthesis of graphene quantum dots: Weigh 2.0g of anhydrous citric acid in a 250mL beaker, place it in an electrochemical oven and heat to 200°C, keep the temperature for 30min, and observe that the citric acid turns into a pale yellow liquid, take it out, and put it into a light yellow liquid. Add 100 mL of sodium hydroxide with a concentration of 10 mg / mL dropwise, and stir vigorously while adding dropwise; finally neutralize with sodium hydroxide to pH=7.0 to obtain an aqueous solution of graphene quantum dots; store in a refrigerator at 4°C in the dark, and dilute 8 times;

[0046] from Figure 2A It can be seen that the particle size distribution of the prepared graphene quantum dots is relatively uniform, and there is strong blue fluorescence under the 365nm UV lamp. The optimal excitation wavelengt...

Embodiment 2

[0055] The present embodiment provides a ratiometric fluorescence analysis method for detecting nitrite, which specifically includes the following steps:

[0056] Step S21. Synthesis of graphene quantum dots: Weigh 2.0g of anhydrous citric acid in a 250mL beaker, place it in an electrochemical oven and heat it to 200°C, keep the temperature for 1h, and observe that the citric acid turns into a light yellow liquid, take it out, and put it into a light yellow liquid. Add 100 mL of sodium hydroxide with a concentration of 8 mg / mL dropwise, stir vigorously while adding dropwise; finally neutralize it with sodium hydroxide to pH=6.5 to obtain an aqueous solution of graphene quantum dots; store it in a refrigerator at 4°C in the dark, and dilute it by 10 when in use. times;

[0057] Step S22. Establishment of a standard curve: use 0.05M hydrochloric acid to prepare standard solutions of sodium nitrite with different concentrations (1.29×10 -5 ~6900mg / kg) and o-phenylenediamine solu...

Embodiment 3

[0060] The present embodiment provides a ratiometric fluorescence analysis method for detecting nitrite, which specifically includes the following steps:

[0061] Step S31. Synthesis of graphene quantum dots: Weigh 2.0g of anhydrous citric acid in a 250mL beaker, place it in an electrochemical oven and heat to 200°C, keep the temperature for 30min, and observe that the citric acid turns into a pale yellow liquid, take it out, and put it into a light yellow liquid. Add 100 mL of sodium hydroxide with a concentration of 5 mg / mL dropwise, and stir vigorously while adding; finally neutralize it with sodium hydroxide to pH=7.5 to obtain an aqueous solution of graphene quantum dots; store it in a refrigerator at 4°C in the dark, and dilute 6 times;

[0062] Step S32. Establishment of a standard curve: use 0.5M hydrochloric acid to prepare standard solutions of sodium nitrite with different concentrations (1.29×10 -5 ~6900mg / kg) and o-phenylenediamine solution with a concentration o...

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Abstract

The invention discloses a ratio fluorescence analysis method for detecting nitrite, which comprises the following steps: S1. Synthesis of graphene quantum dots; S2. Establishment of standard curve; S3. Determination of nitrite in a sample. The invention generates benzotriazole (BPA) by utilizing sodium nitrite and o-phenylenediamine (OPD) to undergo derivatization reaction under acidic conditions, and the benzotriazole (BPA) produces strong fluorescence in an alkaline medium. As a good fluorescence quencher and response signal at the same time, BPA has a characteristic absorption at 450nm, which can efficiently quench the fluorescence (reference signal) of graphene quantum dots (Ex: 350nm, Em: 578nm), so as to achieve A ratiometric fluorescence analysis method was developed for the detection of trace amounts of nitrite. This method has the advantages of simple operation and high sensitivity (the minimum detection limit can be as low as 1.29×10 ‑5 mg / kg), fast and convenient (reaction time 15min) and other advantages, it can be used for rapid detection of nitrite and has broad application prospects.

Description

technical field [0001] The invention belongs to the field of fluorescence analysis, and particularly relates to a ratio fluorescence analysis method for detecting nitrite based on graphene quantum dots. Background technique [0002] Nitrite generally exists in the environment, food, industry and physiological systems, especially the synthesis and metabolism of nitrite in organisms all the time. In recent years, the misuse of agricultural nitrogen fertilizers and the mismanagement of natural resources have led to disturbances in the local and global nitrogen cycle. The high solubility and mobility of nitrite ions in soil, coupled with the continuous reliance on inorganic fertilizers in agriculture, lead to the continuous loss of nitrite ions, which seriously pollutes adjacent water bodies. Coupled with the discharge of industrial wastewater, the nitrite pollution of underground drinking water resources is becoming more and more serious. At the same time, nitrite is also a c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 罗林廖彩霞徐振林贾宝珠华夏周凯孙远明王弘王烨叶
Owner SOUTH CHINA AGRI UNIV
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