Drug residue competition-type quantum dot-labeled immunochromatography assay test-strip and observation device thereof

A technology of quantum dots and test strips, which is applied in the field of drug residue competitive quantum dot labeling immunochromatography detection card and its observation device, achieving the effects of high sensitivity, simple detection device and low cost

Inactive Publication Date: 2009-10-07
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the small molecule to be tested has only a single epitope, once it binds to antibody 1, it is difficult for antibody 2 to specifically bind to the small molecule

Method used

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  • Drug residue competition-type quantum dot-labeled immunochromatography assay test-strip and observation device thereof
  • Drug residue competition-type quantum dot-labeled immunochromatography assay test-strip and observation device thereof
  • Drug residue competition-type quantum dot-labeled immunochromatography assay test-strip and observation device thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0048] 3. Preparation of immunochromatographic detection card

[0049] On a single-sided polyester or plastic plate, paste sample pad 1, glass fiber membrane 2 coated with quantum dot-labeled antibody, nitrocellulose membrane or cellulose acetate membrane coated with antibody in parallel, and water-absorbing pad 5 and pH Test paper, each layer must be closely connected, after pasting, cut into 3-5mm wide test paper strips, paste the precision pH test paper with a color change range of 5.5-9.0 on the absorbent paper, and then get the immunochromatography test strip 11 , assembled into the test card package after drying, and stored in the dark at 4-25°C.

[0050] 4. Sample testing and result judgment

[0051] Use a dropper to drop the sample liquid to be tested in the sample tank, insert it into the slot 16 of the observation device after the color of the end point indicator window 13 changes, and observe the detection zone 3 and Fluorescence intensity changes of quality contr...

Embodiment 1

[0055] NaBH 4 Dissolve 0.5g and 1.68g of Te powder in 20ml of distilled water, react under nitrogen protection to form NaHTe, then adjust the pH value to 8.5 with concentrated ammonia water, add CdCl 2 2.5H 2O 1.5g, water bath 90°C, magnetic stirring, heating to reflux. Within 80 minutes of reaction time, samples of aqueous solution containing CdTe were taken out at regular intervals. The particle sizes of quantum dots obtained at different reaction times were different. After centrifugal sedimentation, they were washed with distilled water for 4 times to obtain CdTe quantum dots. CdTe was made into 0.5 mg / ml in water. Then, under the protection of Ar, the Zn precursor and the S precursor were slowly added to the CdTe quantum dot solution to a final concentration of 0.45 mg / ml and 0.16 mg / ml (freshly prepared 220 mg / ml zinc acetate and 1 mg / ml sulfurized Sodium aqueous solution), magnetic stirring, reflux in a water bath at 100°C, and after full reaction, yellow to pink co...

Embodiment 2

[0062] 1.2mL 0.15mol / L CdSO 4 Dilute with about 20mL of deionized water, inject 0.6mL of 2.0% trisodium citrate solution into this solution, and then add 0.75mL of 0.2mol / L Na 2 S was added to the mixture for reaction, and samples of the CdS solution were taken at regular intervals. The quantum dots obtained at different reaction times had different particle sizes, and were demulsified and washed with acetone, centrifuged, and the precipitate was collected to obtain the product CdS quantum dots. NaBH 4 0.5g and 0.5g of Se powder were dissolved in 20ml of distilled water, reacted to generate NaHSe under the protection of nitrogen, then adjusted the pH value to 8.8 with concentrated ammonia water, then mixed 20mmol / L ZnCl and 20mmol / L mercaptopropionic acid in equal volume ratio, adjusted When the pH value reaches 11, the NaHSe solution is injected to a concentration of 3 mmol / L to obtain a ZnSe solution. Then the CdS quantum dot solution was slowly added to the ZnSe solution...

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Abstract

The invention provides a drug residue competition-type quantum dot-labeled immunochromatography assay test-strip/detection card and an observation device thereof, comprising the following preparation steps of coating the antibody of quantum dot-labeled drug molecule on a fiberglass film, coating the drug-molecular-carrier protein coupler and second antibody on a pyroxylin film or a cellulose acetate film respectively to form a detection belt and a quality control belt; preparing the immunochromatography assay test-strip from the fiberglass film and the pyroxylin film or the cellulose acetate film on a polyester or plastic plate, and assembling a shell. The quantum dots of the detection belt and the quality control belt on the test-strip is triggered by the ultraviolet LED source of the observation device, and the change of the fluorescent intensity of the observation belt and the quality control belt is observed, thus being capable of quantitatively analyzing the content of the drug molecule in the sample. The invention has simple operation, high sensitiveness and quick quantification, is suitable for detecting the drug residue in the food and can be widely applied to the customhouse, airport, health supervising department, household and the like.

Description

technical field [0001] The invention relates to rapid biological detection technology, in particular to a drug residue competition type quantum dot label immunochromatographic detection card and an observation device thereof. Background technique [0002] With the modernization, intensification, large-scale and commercial production of animal husbandry, antibiotics and feed additives have played a very significant role in reducing animal morbidity and mortality, improving feed utilization, and promoting growth. However, driven by economic interests, the abuse of antibiotics and excessive use of additives in production have caused the residues of veterinary drugs in livestock and poultry products to have seriously affected the quality and hygiene safety of animal foods, posing a threat to people's lives. For allergies, bacterial resistance, triple effects (carcinogenic, teratogenic, mutagenic) and hormone (like) effects. In addition to food safety reasons, to deal with green...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558
Inventor 胡华军洪治刘军张明洲俞晓平
Owner CHINA JILIANG UNIV
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