High voltage electrospinning method for preparing multi-fluorescence-encoded micro-beads

A technology of encoding microspheres and multiple fluorescence, which is applied in the field of fluorescence encoding, can solve the problems of difficulty in adding a large amount, tediousness, and difficulty in the addition process, and achieve good application and promotion value, simple operation, and low cost.

Inactive Publication Date: 2009-01-07
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to their estimation, quantum dots with 1 wavelength and 10 intensities (0, 1, 2...9) can produce 9 codes, that is, 10 1 -1, with 3 wavelengths and 10 intensities (0, 1, 2...9), there can be 999 codes, that is, 10 3 -1 type, so quantum dots with m wavelengths and n intensities (0, 1, 2...n-1) can have n m -1 code, so as long as the quantum dot materials with 10 colors and 6 intensities are used, the number of codes theoretically obtained can reach millions, except for some microspheres that cannot be accurately detected due to spectral overlap, the available quantum dots The number of material microspheres can reach more than 40,000, but this method is cumbersome to operate. First, it is necessary to prepare polystyrene microspheres with a suitable pore size, and then swell them in a solvent, and then in order from large to small. The dot material is absorbed into the interior of the microsphere; at the same time, with the increase of the amount of quantum dot material added, the later adding process becomes more difficult, and it is difficult to achieve a large amount of theoretical addition

Method used

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  • High voltage electrospinning method for preparing multi-fluorescence-encoded micro-beads
  • High voltage electrospinning method for preparing multi-fluorescence-encoded micro-beads

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: polystyrene microspheres containing monochromatic CdSe / ZnS quantum dot material

[0043] Mix and dissolve 60g of styrene, 6g of acrylic acid, and 0.07g of azobisisobutyronitrile in tetrahydrofuran, heat at 70°C for 10 hours, then settle, wash and dry to obtain polystyrene, which is set aside;

[0044] Dissolve 2g of polystyrene in 18g of N,N-dimethylformamide to make its concentration 10%;

[0045] will have a maximum absorption at 612nm of 2×10 -4 g CdSe / ZnS (U.S. Ocean Nanotech product) quantum dot material ultrasonically dispersed in 5g chloroform;

[0046] Mix 10g polystyrene N,N-dimethylformamide solution with 5g CdSe / ZnS chloroform solution, stir evenly, and ultrasonically disperse the quantum dot material in the polystyrene solution for 30 minutes, then add the solution to In the spinning tube of the electrospinning equipment, a nozzle with a diameter of 1 mm is installed, and the nozzle is connected to the positive electrode of the high-voltage p...

Embodiment 2

[0047] Embodiment 2: polystyrene microspheres containing two-color CdSe / ZnS quantum dot material

[0048] The preparation of polystyrene solution is as shown in embodiment 1;

[0049] will have a 4×10 maximum absorption at 612nm -4 g CdSe / ZnS quantum dot material ultrasonically dispersed in 5g chloroform;

[0050] will have a maximum absorption of 3.8×10 at 586nm -4 g CdSe / ZnS quantum dot material ultrasonically dispersed in 5g chloroform;

[0051] Mix 10g polystyrene solution with 2.5g CdSe / ZnS (612nm) and 2.5g CdSe / ZnS (586nm) chloroform solution of quantum dot material, stir for 5h, and ultrasonically disperse quantum dot material in polystyrene solution for 30 minutes , and then put the solution into the spinning tube, add a nozzle with a diameter of 1 mm, and connect the nozzle to the positive electrode of the high-voltage power supply as the anode, use aluminum foil as the negative electrode, adjust the distance between the positive and negative electrodes to be 10cm,...

Embodiment 3

[0054] Example 3: Polystyrene microspheres containing three-color CdSe / ZnS quantum dots

[0055] The preparation method of polystyrene is implemented as embodiment 2 method;

[0056] will have a 4×10 maximum absorption at 612nm -4 g CdSe / ZnS quantum dot material ultrasonically dispersed in 5g chloroform;

[0057] will have a maximum absorption of 3.8×10 at 586nm -4 g CdSe / ZnS quantum dot material ultrasonically dispersed in 5g chloroform;

[0058] will have a maximum absorption of 3.3×10 at 530nm -4 g CdSe / ZnS quantum dot material ultrasonically dispersed in 5g chloroform;

[0059] Dissolve 2.0g of polystyrene in 15.5g of N,N-dimethylformamide, stir and set aside;

[0060] Get the polystyrene solution of 10g preparation, then add the chloroform solution of 2.5g CdSe / ZnS (618nm), 2.5gCdSe / ZnS (586nm), 2.5g CdSe / ZnS (530nm) quantum dot material, then adopt the implementation of embodiment 2 The corresponding polystyrene multicolor microspheres are prepared in the process, ...

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Abstract

The invention belongs to the fluorescence-coded technology field and in particular relates to a high voltage electric spinning preparation method of multiplex fluorescence coding microsphere. In the method, fluorescent materials (quantum dots material or fluorescent dyes) with different quantities and different fluorescent characteristics are dispersed in polymer solution (or inorganic matter sol). Then, through the high voltage electric spinning process, the coding microsphere with controllable size and adjustable fluorescent strength and lighting wavelength is obtained. The quantum dots material can be used alone or used in mixing way. The fluorescent material can be used alone or used in mixing way. The flurescent material can also be used together with the quantum dots material. The coding microsphere prepared through the invention can provide fluorescent probes in large quantities for the fields of gene expression, protein-protein interaction, simultaneous detection of various diseases, high throughput screening, combinational chemistry, etc. The method of the invention has the advantages of simple operation, wide application, low cost, stable fluorescence performance, and the like, and is provided with good application and expansion value.

Description

technical field [0001] The invention belongs to the technical field of fluorescent coding, and in particular relates to a high-voltage electrospinning preparation method of multiple fluorescent coding microspheres. Background technique [0002] In recent years, with the development of genomics and proteomics research, especially the completion of human genome sequencing, a huge amount of parallel sequence data has been generated, and it is urgent to find a fast and effective detection and analysis technology for such a large number of Nucleic acid and protein screening, selection of fluorescent / polymer microspheres with multiple spectral characteristics (multi-wavelength and multi-intensity) for parallel encoding and labeling of biological macromolecules is an effective means to solve this problem, because theoretically, different intensities The combined use of fluorescent substances of different colors can produce thousands of optically encoded polymer microspheres, and bi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C08J3/12G01N21/64G01N33/52
Inventor 杨清彪宋岩李耀先王永强战乃乾
Owner JILIN UNIV
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