Multifunctional nanomicelle for early diagnosis and phototherapy of tumors and application of multifunctional nanomicelle

A nano-micelle, early diagnosis technology, applied in preparations for in vivo experiments, medical preparations containing active ingredients, wave energy or particle radiation treatment materials, etc., can solve the problem of losing the chance of surgical treatment and clearing the reticuloendothelial system , strong hydrophobicity and other issues, to achieve the effects of improving drug systemic circulation time, improving imaging signal intensity, and good biocompatibility

Active Publication Date: 2015-11-04
榕知科技(武汉)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of liver cancer is still dominated by surgical resection, but many patients lose the chance of surgical treatment due to the influence of tumor location, physical tolerance status and degree of liver cirrhosis when they are diagnosed
Although local chemotherapy, interventional therapy, radiofrequency ablation therapy, immunotherapy and even liver transplantation have been widely used in the comprehensive treatment of liver cancer and have achieved remarkable curative effects,

Method used

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  • Multifunctional nanomicelle for early diagnosis and phototherapy of tumors and application of multifunctional nanomicelle
  • Multifunctional nanomicelle for early diagnosis and phototherapy of tumors and application of multifunctional nanomicelle
  • Multifunctional nanomicelle for early diagnosis and phototherapy of tumors and application of multifunctional nanomicelle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Weigh 4.2mg of DSPE-PEG-NH with an electronic balance 2 (Polyethylene glycol-distearoylphosphatidylethanolamine, Nanocs company) white powder and 4.2mg of DOTA-NHS white powder (gadolinium ion chelating agent, Macrocyclic company) white powder, dissolved in 1.5ml of 1ml aqueous solution In a centrifuge tube, after shaking and reacting at room temperature for 2 hours, transfer to a refrigerator at 4°C, shake and react for 24 hours, and then filter with a 5KDa ultrafiltration tube to collect DSPE-PEG-DOTA-NHS, and then add 60 mg of GdCl to it 3 (Gadolinium trichloride, Bailingwei Reagent Co., Ltd.) was a white powder. After being strongly shaken for 12 hours, it was filtered with a 5KDa ultrafiltration tube to collect DSPE-PEG-Gd-DOTA, and it was freeze-dried using a low-temperature freeze dryer for future use.

Embodiment 2

[0031] 0.25 mg of poly[2,1,3-benzothiadiazole-4,7-diyl[4,4-bis(2-ethylhexyl)-4H-cyclopenta[2,1-b: 3,4-b']dithiophene-2,6-diyl]](PCPDTBT) (Sigma, USA) was dissolved in 1 mL of THF, and 0.5 mg of chlorin e6 (Chlorin e6) powder was added to it , 2.5 mg of hydrogenated soybean lecithin (HSPC, Shanghai Avete Pharmaceutical Technology Co., Ltd.), 0.5 mg of DSPE-PEG-Gd-DOTA powder, after mixing them evenly, quickly inject 10 mL of Milli-Q ultrapure water (USA Millipore Company), utilize the ultrasonic probe of 40W to carry out sonication 3min, then the mixed solution is poured in the flask of 50ml, add magneton and carry out under 500rpm, room temperature, dark stirring overnight, remove the THF solvent in the mixed solution. Afterwards, dialysis was performed for 3 days using a 10KDa dialysis bag to remove excess Chlorin e6 molecules and Pdots to obtain Pdots / Ce6lipid-Gd-DOTA multifunctional nanomicelles.

Embodiment 3

[0033] The particle diameter of the Pdots / Ce6lipid-Gd-DOTA nanomicelle prepared in Example 2 is about 36±12nm, and has the functions of MRI, photoacoustic imaging, near-infrared imaging, photodynamic and photothermal therapy.

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Abstract

The invention relates to a multifunctional nanomicelle for early diagnosis and phototherapy of tumors. The multifunctional nanomicelle comprises a monolayer functionalized lipid membrane shell, photosensitive molecules and optothermal absorbents, wherein the photosensitive molecules and the optothermal absorbents are embedded into the monolayer functionalized lipid membrane shell. The multifunctional nanomicelle has the advantages that the nanomicelle can be used for multi-modal imaging diagnosis such as early magnetic resonance imaging, photoacoustic imaging or near-infrared fluorescence imaging of cancer; multiple kinds of diagnosis effects can be achieved as long as a patient suffers from one contrast medium administration; since sensitivity is improved, contrast medium injecting dosage is further decreased, and toxic and side effects on the patient can be alleviated; when the tumors are under single-wavelength near-infrared laser radiation, chlorine 6 and Pdots inside the nanomicelle are capable of converting light energy into chemical energy and thermal energy effectively to produce a great number of ROS (reactive oxygen species) oxygen free radicals and overhigh heat at 50-60 DEG C, and the ROS oxygen free radicals and the overhigh heat are used for inducing apoptosis and necrosis of cancer cells, so that a photodynamic and photothermal collaborative therapy function is achieved; the multifunctional nanomicelle not only can be used as a contrast medium for cancer diagnosis, but also can be used as a therapeutic agent for collaborative therapy of the cancer.

Description

(1) Technical field [0001] The invention relates to a Pdots / Ce6lipid-Gd-DOTA multifunctional nano-micelle for early tumor diagnosis and optical therapy and application thereof. (2) Background technology [0002] Hepatocellular carcinoma is one of the common malignant tumors in the digestive system. It has the characteristics of rapid development, high degree of malignancy and poor prognosis, and its incidence is increasing year by year. As a high-incidence malignant tumor, the cancer mortality rate of liver cancer ranks first among all malignant tumors in Fujian Province, about 32 people per 100,000 people died of liver cancer. At present, the treatment of liver cancer is still based on surgical resection, but many patients lose the chance of surgical treatment due to the influence of tumor location, physical tolerance status and degree of liver cirrhosis when they are diagnosed. Although local chemotherapy, interventional therapy, radiofrequency ablation therapy, immunothe...

Claims

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Application Information

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IPC IPC(8): A61K49/12A61K49/00A61K41/00
Inventor 刘小龙刘景丰张达曾永毅
Owner 榕知科技(武汉)有限公司
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