Monoamine oxidase activity fluorescent detecting method

Abstract

The invention relates to a fluorescent testing method of monoamine oxidase activity. It forms fluorescent material, detects fluorescent intensity and achieves monoamine oxidase activity data by using monoamine oxidase to hydrolyze fluorescent probe. The probe is coumarin compound and its structure as shown in (I), in which R1 and R2 are alkyl of H or C1-C6 independently. Preparation of probe in this invention just needs simple device, convenient operation and easily gained materials. The prepared probe has stable property and high purity (above 99%). Compared to the current method for testing monoamine oxidase activity, its accuracy, sensitivity and testing velocity are all improved.

Description

(1) Technical field

[0001] The invention relates to a fluorescence detection method of monoamine oxidase activity, in particular to a fluorescence detection method of monoamine oxidase activity using coumarin compounds as fluorescent probes. (2) Background technology

[0002] Monoamine oxidase is a type of mitochondrial membrane-bound enzyme, which catalyzes the oxidative deamination of amine compounds in organisms to generate corresponding aldehydes in the brain and peripheral nervous tissue. Studies have shown that during the process of metabolizing dopamine, monoamine oxidase produces endogenous substances such as free radicals and hydrogen peroxide, and its abnormal expression is closely related to Parkinson's disease and depression. In addition, the determination and analysis of monoamine oxidase activity in the blood of patients is of great value for the early diagnosis of acute and chronic hepatitis and liver cirrhosis. Therefore, finding a highly sensitive detection...

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