Integral detection reaction plate and protein chip kit of hapetitis and cirrhosis

A technology integrating detection and reaction plates, applied in the field of in vitro clinical testing and biochips, can solve problems such as difficulty in meeting the inspection market, unfavorable routine physical examination, popularization of civilians, and cost.

Inactive Publication Date: 2006-11-29
穆海东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] The current serological detection method is mainly based on ELISA method, which can only detect a sing...

Method used

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  • Integral detection reaction plate and protein chip kit of hapetitis and cirrhosis
  • Integral detection reaction plate and protein chip kit of hapetitis and cirrhosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Preparation of detection reaction plate

[0044] Take a 96-well plate and set 42-84 sample wells and 1-8 standard wells. A solid phase support (NC membrane or PDVF membrane) is placed at the bottom of each reaction well, and the solid phase support is coated with six kinds of antigens such as anti-HBsAg, HCVAg, anti-AFP, anti-AFU, anti-MAO, and anti-HA. Antibody protein microarray ( figure 1 ).

Embodiment 2

[0046] kit preparation

[0047] Prepare a test kit containing six indicators integrated detection of hepatitis B and C, liver cirrhosis and liver cancer, which contains one reaction well plate (8-200 people) of embodiment 1, 1 bottle of washing concentrate, 1 bottle of enzyme-labeled working solution One bottle of detection solution A, one bottle of detection solution B, and a standard bottle.

[0048] Washing Concentrate, Enzyme Label Working Solution, Detection Solution A, Detection Solution B

Embodiment 3

[0050] Integrated detection of six indicators of hepatitis B and C, liver cirrhosis and liver cancer

[0051] Test each blood sample according to the following method:

[0052] 1. Add test blood or standard products to corresponding reaction wells, 100ul / well.

[0053] 2. Add enzyme-labeled working solution, 75ul / well.

[0054] 3. Incubate and shake at 30°C for 60 minutes.

[0055] 4. Wash 4-6 times with lotion, 5 minutes each time, vibrate.

[0056] 5. Add detection solution 40ul / well, so that the detection solution is evenly distributed on the bottom of the well.

[0057] 6. CCD detection, exposure for 3-20 seconds depending on the intensity of the luminescent signal.

[0058] Result judgment:

[0059] According to the CutOff value of different indicators, refer to the gradient curve of the standard to determine the negative or positive of the sample serum.

[0060] At the same time, the serum samples tested were routinely tested by conventional methods. The result sh...

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Abstract

The invention relates to an integrated detection reaction orifice plate and protein chip reagent box for detecting six indexes of diagnosis, forecast and prognosis of hepatitis, hepatocirrhosis and liver cancer, including hepatitis B virus surface antigen (HBsAg), hepatitis C virus antibody (HCVAb), alpha-fetoprotein (AFP), alpha-L-Fucosidase (AFU), mono amine oxidase (MAO), hyaluronic acid (HA). And the orifice plate comprises a substrate and reaction orifices on the substrate, where the reaction orifices comprise 2-384 sample orifices and 2-8 standard product orifices, and at the bottom of each reaction orifice is solid carrier coated with micro lattice of HBsAg, HCVAg, AFP, AFU,MAO,and HA antigens /antibodies or more. And the reaction plate and reagent can simply and conveniently, quickly and accurately implement simultaneous detection of the six indexes of diagnosis, forecast and prognosis of hepatitis, hepatocirrhosis and liver cancer for many persons.

Description

technical field [0001] The invention belongs to the field of in vitro clinical testing and biochip technology, specifically, it is a six-item index (HBsAg, HCVAb, AFU, AFP, MAO, and HA) that is applicable to the diagnosis, prediction, and prognosis of hepatitis, liver cirrhosis, and liver cancer. ) Reaction plates and kits for integrated detection. Background technique [0002] Viral hepatitis is a statutory Class B infectious disease, which has the characteristics of strong infectivity, complicated transmission routes, wide prevalence and high incidence; some patients with hepatitis B and C can develop into chronic diseases, and can develop into liver cirrhosis and primary disease. Hepatocellular carcinoma is very harmful to people's health. [0003] my country is a major hepatitis B country, and 75% of the world's hepatitis B patients are concentrated in my country. More than 600 million people have been infected with hepatitis B, about 130 million people carry hepatitis...

Claims

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Application Information

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IPC IPC(8): G01N33/576G01N33/547G01N33/52
Inventor 穆海东
Owner 穆海东
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