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Lactobacillus rhamnosus and application thereof

A technology of Lactobacillus rhamnosus and its application, which is applied in the field of Lactobacillus rhamnosus and its application, can solve the problems of lack of high-yield monoamine oxidase inhibitor strains and other problems, and achieve the improvement of human health, improve the quality of human life, and huge social benefits and economical effects

Active Publication Date: 2013-03-27
BRIGHT DAIRY & FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the technical problem to be solved by the present invention is to provide a kind of Lactobacillus rhamnosus (Lactobacillus rhamnosus) with high production of monoamine oxidase inhibitors and its application in view of the lack of high-production monoamine oxidase inhibitor strains at present.

Method used

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  • Lactobacillus rhamnosus and application thereof
  • Lactobacillus rhamnosus and application thereof
  • Lactobacillus rhamnosus and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Isolation and screening of embodiment 1 lactic acid bacteria

[0030] 1. Isolation of Lactic Acid Bacteria

[0031] Collection of samples The samples were taken from naturally fermented kefir milk in Xinjiang. The samples were put into sterile screw-top bottles, and the caps were tightened tightly to carry out the screening operation of lactic acid bacteria.

[0032] Separation from MRS plates Take 1 mL of the sample and shake it in 100 mL of sterile PBS (pH 6.8) buffer solution for about 10 min to make it fully mixed. Draw 100 μL of the sample, carry out ten-fold serial dilution, draw 10 -4 100 μL of diluted solution was evenly spread on the MRS (Merck, Germany) screening agar plate, cultured anaerobically at 37°C for 48 hours, observed the growth of the colony, and performed Gram staining on the single colony preparation, and picked Gram Positive bacteria, as the primary target strain, were transferred to the MRS plate for purification and culture, and stored at 4°C...

Embodiment 2

[0049] Example 2 Preparation of concentrated product of Lactobacillus rhamnosus KF7 fermented milk

[0050] 1. Preparation of Lactobacillus rhamnosus KF7 fermented milk

[0051] Activation of the strain: take out the preserved Lactobacillus rhamnosus KF7 strain from the -80°C deep freezer, and thaw at room temperature. Under sterile conditions, take a little freeze-dried bacterial powder and dissolve it in 1mL sterile saline (0.85% NaCl solution), dip a little in the inoculation loop, streak and separate on the MRS (Merck, Germany) plate, anaerobic at 37°C Cultured for 48h (N 2 :H 2 :CO 2 =85:10:5), activated twice in a row.

[0052] Preparation of seeds: 10% (w / w) skim milk, sterilized at 118°C for 15 minutes. Pick a small amount of bacterial lawn from a freshly cultivated MRS plate, transfer it to 1mL of 10% (w / w) sterile skim milk, and culture it at 37°C for 24 hours, and then press the inoculation amount of 2% (v / v) in the 10% (w / w) sterile skim milk was further expa...

Embodiment 3

[0057] Example 3 Detection of monoamine oxidase inhibitory activity of Lactobacillus rhamnosus KF7 fermented milk crude concentrate

[0058] The fermented milk crude concentrate obtained in Step 2 of Example 2 was formulated into a 100 mg / mL solution, and diluted to 50, 25, 12.5, 6.25, 3.125, 1.5625 mg / mL, and tested according to the method described in Example 1. Monoamine oxidase inhibitory activity.

[0059] The test results showed that the monoamine oxidase inhibitory rate of the crude concentrated product of Lactobacillus rhamnosus KF7 fermented milk increased with the increase of its concentration. 28.9% rose to 63.2%, IC 50 About 23.5mg / mL.

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Abstract

The invention discloses lactobacillus rhamnosus and application thereof. The lactobacillus rhamnosus is preserved in the China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC No.6430. Raw milk is used as a growth medium of the strain, and the monoamine oxidase inhibition rate of the obtained fermented milk can reach 52.4-78.8%. The lactobacillus rhamnosus can be used as a functional additive for producing beverages and dietary supplements with the functions of inhibiting the monoamine oxidase activity, fighting Parkinson's disease, easing depression or defying aging, and can also be applied to preparation of drugs for fighting Parkinson's disease or easing depression.

Description

technical field [0001] The invention relates to a novel Lactobacillus rhamnosus and its application in preparing food, health products or medicines. Background technique [0002] Population aging is a major problem facing mankind in the 21st century. Aging-related diseases, such as Alzheimer's disease, Parkinson's disease, etc., have gradually become the enemy of human health and a heavy burden on social prosperity. At present, with the expansion of the aging population, functional foods that can provide probiotics, control aging and prolong life are becoming more and more popular. Lactobacillus, especially Lactobacillus, as a kind of edible fungus recognized as safe, has been continuously discovered and confirmed to regulate and promote human health, and its potential anti-aging effect has gradually attracted widespread attention. In recent years, the functions of probiotics to improve cardiovascular health, such as lowering blood fat, antihypertensive, and lowering chole...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123A23L1/29A61K35/74A61P25/16A61P25/24C12R1/225A23L33/00A61K35/747
Inventor 季红吴正钧艾连中游春苹郭本恒韩瑨陈卫
Owner BRIGHT DAIRY & FOOD CO LTD
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