Industrial production method of intestinal tract probiotic preparation

Abstract

The invention provides an industrial production method of an intestinal tract probiotic preparation. The industrial production method comprises the following steps of (1) performing mass culture on lactobacillus casei, lactobacillus rhamnosus, bifidobacterium bifidum, lactobacillus acidophilus, streptococcus thermophilus, lactobacillus delbrueckii subspecies bulgaricus and the like through a fermenter; (2) performing centrifuging and washing with sterile water to obtain wet bacterium paste, and uniformly mixing the wet bacterium paste with a special protective agent in the ratio being 1 to 1;and (3) granulating a mixture of the wet bacterium paste and the protective agent, performing rolling to obtain balls, performing low-temperature vacuum drying, and performing coating so as to obtainthe intestinal tract probiotic preparation. Under the culture condition provided by the invention, probiotics can grow and propagate at high density, the collected wet bacterium paste does not need tobe subjected to freeze drying, the wet bacterium paste is directly subjected to vacuum low-temperature quick drying with the special protective agent, and massive bacterium bodies can be maintained to be alive; and energy resources are saved, and the production cost is reduced.

Description

technical field

[0001] The invention relates to the field of microbial preparations, in particular to an industrial production method of intestinal probiotic preparations. Background technique

[0002] Probiotics refer to single or well-defined mixed microorganisms that change the composition of the flora in a certain part of the host through colonization, thereby producing a beneficial effect on the health of the host. It has beneficial physiological effects on the host by regulating the host mucosal and systemic immune function and improving the balance of intestinal nutrition and flora. Probiotics can be roughly divided into three categories, including Lactobacillus (such as Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus jansnii, Lactobacillus Lamansii, etc.), Bifidobacterium (such as Bifidobacterium longum, Bifidobacterium breve, ovale, bifidobacterium thermophile, etc.), gram-positive cocci (such as faecal streptococcus, lactococcus, intermediary strepto...

Examples

Embodiment 1

[0031] 1. Fermentation and cultivation of Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum, Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus delbrueckii subsp. Add 2% agar in the base, cultivate for 24 hours, then insert in the Erlenmeyer flask liquid culture medium (the culture medium formula is the same as that of the large tank culture medium) and cultivate separately, cultivate for 24 hours at 37°C, and then expand the cultivation step by step, and then Then carry out fermentation and culture in large tanks separately, culture at 37°C, and stir properly during the culture process; after 24 hours of culture, detect the total number of probiotics in the fermentation liquid, when the number of bacteria in the fermentation liquid reaches 1.5×10 9 cfu / mL, end the culture;

[0032] The formula of the large tank medium is: soybean peptide 2.0g, whey powder 5.0g, glucose 10.0g, lactose 2.0g, trypsin hydrolyzed casein 5.0g, beef extract 2.0g...

Embodiment 2

[0038] 1. Fermentation and cultivation of Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum, Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus delbrueckii subsp. Add 2% agar in the base, cultivate for 20 hours, then insert in the Erlenmeyer flask liquid culture medium (the culture medium formula is the same as the large tank culture medium) and cultivate respectively, cultivate for 20 hours at 37°C, then expand and cultivate step by step, and then Then carry out fermentation and culture in large tanks separately, culture at 37°C, and stir properly during the culture process; after 24 hours of culture, detect the total number of probiotics in the fermentation liquid, when the number of bacteria in the fermentation liquid reaches 2.0×10 9 cfu / mL, end the culture;

[0039] The formula of the large tank medium is: soybean peptide 3.0g, whey powder 4.0g, glucose 15.0g, lactose 2.5g, trypsin hydrolyzed casein 5.0g, beef extract 5.0g, yeast extr...

Embodiment 3

[0045] 1. Fermentation and cultivation of Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum, Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus delbrueckii subsp. Add 2% agar in the base, cultivate for 24 hours, then insert in the Erlenmeyer flask liquid culture medium (the culture medium formula is the same as that of the large tank culture medium) and cultivate separately, cultivate for 24 hours at 37°C, and then expand the cultivation step by step, and then Then carry out fermentation and culture in large tanks respectively, static culture at 37°C, and stir properly during the culture process; after 24 hours of culture, detect the total number of probiotics in the fermentation liquid, when the number of bacteria in the fermentation liquid reaches 2.5×10 9 cfu / mL, end the culture;

[0046] The formula of the large tank medium is: soybean peptide 5.0g, whey powder 1.0g, glucose 5.0g, lactose 6.0g, trypsin hydrolyzed casein 10g, beef extra...

technical field

[0001] The invention relates to the field of microbial preparations, in particular to an industrial production method of intestinal probiotic preparations. Background technique

[0002] Probiotics refer to single or well-defined mixed microorganisms that change the composition of the flora in a certain part of the host through colonization, thereby producing a beneficial effect on the health of the host. It has beneficial physiological effects on the host by regulating the host mucosal and systemic immune function and improving the balance of intestinal nutrition and flora. Probiotics can be roughly divided into three categories, including Lactobacillus (such as Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus jansnii, Lactobacillus Lamansii, etc.), Bifidobacterium (such as Bifidobacterium longum, Bifidobacterium breve, ovale, bifidobacterium thermophile, etc.), gram-positive cocci (such as faecal streptococcus, lactococcus, intermediary strepto...

Examples

Embodiment 1

[0031] 1. Fermentation and cultivation of Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum, Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus delbrueckii subsp. Add 2% agar in the base, cultivate for 24 hours, then insert in the Erlenmeyer flask liquid culture medium (the culture medium formula is the same as that of the large tank culture medium) and cultivate separately, cultivate for 24 hours at 37°C, and then expand the cultivation step by step, and then Then carry out fermentation and culture in large tanks separately, culture at 37°C, and stir properly during the culture process; after 24 hours of culture, detect the total number of probiotics in the fermentation liquid, when the number of bacteria in the fermentation liquid reaches 1.5×10 9 cfu / mL, end the culture;

[0032] The formula of the large tank medium is: soybean peptide 2.0g, whey powder 5.0g, glucose 10.0g, lactose 2.0g, trypsin hydrolyzed casein 5.0g, beef extract 2.0g...

Embodiment 2

[0038] 1. Fermentation and cultivation of Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum, Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus delbrueckii subsp. Add 2% agar in the base, cultivate for 20 hours, then insert in the Erlenmeyer flask liquid culture medium (the culture medium formula is the same as the large tank culture medium) and cultivate respectively, cultivate for 20 hours at 37°C, then expand and cultivate step by step, and then Then carry out fermentation and culture in large tanks separately, culture at 37°C, and stir properly during the culture process; after 24 hours of culture, detect the total number of probiotics in the fermentation liquid, when the number of bacteria in the fermentation liquid reaches 2.0×10 9 cfu / mL, end the culture;

[0039] The formula of the large tank medium is: soybean peptide 3.0g, whey powder 4.0g, glucose 15.0g, lactose 2.5g, trypsin hydrolyzed casein 5.0g, beef extract 5.0g, yeast extr...

Embodiment 3

[0045] 1. Fermentation and cultivation of Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum, Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus delbrueckii subsp. Add 2% agar in the base, cultivate for 24 hours, then insert in the Erlenmeyer flask liquid culture medium (the culture medium formula is the same as that of the large tank culture medium) and cultivate separately, cultivate for 24 hours at 37°C, and then expand the cultivation step by step, and then Then carry out fermentation and culture in large tanks respectively, static culture at 37°C, and stir properly during the culture process; after 24 hours of culture, detect the total number of probiotics in the fermentation liquid, when the number of bacteria in the fermentation liquid reaches 2.5×10 9 cfu / mL, end the culture;

[0046] The formula of the large tank medium is: soybean peptide 5.0g, whey powder 1.0g, glucose 5.0g, lactose 6.0g, trypsin hydrolyzed casein 10g, beef extra...

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