Industrial production method of intestinal tract probiotic preparation

A technology of intestinal probiotics and probiotics, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low number of viable bacteria in probiotic preparations, death of bacteria, and reduction of the overall vitality of bacteria , to improve the body's immunity, protect the activity, and maintain the balance of intestinal flora

Active Publication Date: 2018-11-13
哈尔滨美华生物技术股份有限公司
View PDF11 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, in the prior art, when the wet bacteria slime is processed, steps such as freeze-drying that require high equipment and high energy consumption are often required, and it w

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Industrial production method of intestinal tract probiotic preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Fermentation and cultivation of Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum, Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus delbrueckii subsp. Add 2% agar in the base, cultivate for 24 hours, then insert in the Erlenmeyer flask liquid culture medium (the culture medium formula is the same as that of the large tank culture medium) and cultivate separately, cultivate for 24 hours at 37°C, and then expand the cultivation step by step, and then Then carry out fermentation and culture in large tanks separately, culture at 37°C, and stir properly during the culture process; after 24 hours of culture, detect the total number of probiotics in the fermentation liquid, when the number of bacteria in the fermentation liquid reaches 1.5×10 9 cfu / mL, end the culture;

[0032] The formula of the large tank medium is: soybean peptide 2.0g, whey powder 5.0g, glucose 10.0g, lactose 2.0g, trypsin hydrolyzed casein 5.0g, beef extract 2.0g...

Embodiment 2

[0038] 1. Fermentation and cultivation of Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum, Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus delbrueckii subsp. Add 2% agar in the base, cultivate for 20 hours, then insert in the Erlenmeyer flask liquid culture medium (the culture medium formula is the same as the large tank culture medium) and cultivate respectively, cultivate for 20 hours at 37°C, then expand and cultivate step by step, and then Then carry out fermentation and culture in large tanks separately, culture at 37°C, and stir properly during the culture process; after 24 hours of culture, detect the total number of probiotics in the fermentation liquid, when the number of bacteria in the fermentation liquid reaches 2.0×10 9 cfu / mL, end the culture;

[0039] The formula of the large tank medium is: soybean peptide 3.0g, whey powder 4.0g, glucose 15.0g, lactose 2.5g, trypsin hydrolyzed casein 5.0g, beef extract 5.0g, yeast extr...

Embodiment 3

[0045] 1. Fermentation and cultivation of Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum, Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus delbrueckii subsp. Add 2% agar in the base, cultivate for 24 hours, then insert in the Erlenmeyer flask liquid culture medium (the culture medium formula is the same as that of the large tank culture medium) and cultivate separately, cultivate for 24 hours at 37°C, and then expand the cultivation step by step, and then Then carry out fermentation and culture in large tanks respectively, static culture at 37°C, and stir properly during the culture process; after 24 hours of culture, detect the total number of probiotics in the fermentation liquid, when the number of bacteria in the fermentation liquid reaches 2.5×10 9 cfu / mL, end the culture;

[0046] The formula of the large tank medium is: soybean peptide 5.0g, whey powder 1.0g, glucose 5.0g, lactose 6.0g, trypsin hydrolyzed casein 10g, beef extra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an industrial production method of an intestinal tract probiotic preparation. The industrial production method comprises the following steps of (1) performing mass culture on lactobacillus casei, lactobacillus rhamnosus, bifidobacterium bifidum, lactobacillus acidophilus, streptococcus thermophilus, lactobacillus delbrueckii subspecies bulgaricus and the like through a fermenter; (2) performing centrifuging and washing with sterile water to obtain wet bacterium paste, and uniformly mixing the wet bacterium paste with a special protective agent in the ratio being 1 to 1;and (3) granulating a mixture of the wet bacterium paste and the protective agent, performing rolling to obtain balls, performing low-temperature vacuum drying, and performing coating so as to obtainthe intestinal tract probiotic preparation. Under the culture condition provided by the invention, probiotics can grow and propagate at high density, the collected wet bacterium paste does not need tobe subjected to freeze drying, the wet bacterium paste is directly subjected to vacuum low-temperature quick drying with the special protective agent, and massive bacterium bodies can be maintained to be alive; and energy resources are saved, and the production cost is reduced.

Description

technical field [0001] The invention relates to the field of microbial preparations, in particular to an industrial production method of intestinal probiotic preparations. Background technique [0002] Probiotics refer to single or well-defined mixed microorganisms that change the composition of the flora in a certain part of the host through colonization, thereby producing a beneficial effect on the health of the host. It has beneficial physiological effects on the host by regulating the host mucosal and systemic immune function and improving the balance of intestinal nutrition and flora. Probiotics can be roughly divided into three categories, including Lactobacillus (such as Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus jansnii, Lactobacillus Lamansii, etc.), Bifidobacterium (such as Bifidobacterium longum, Bifidobacterium breve, ovale, bifidobacterium thermophile, etc.), gram-positive cocci (such as faecal streptococcus, lactococcus, intermediary strepto...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A23L33/135C12N1/20C12R1/245C12R1/225C12R1/01C12R1/23C12R1/46
CPCA23L33/135C12N1/20A23V2002/00A23V2400/125A23V2400/137A23V2400/113A23V2400/175A23V2400/249A23V2400/517A23V2200/3204A23V2200/3262A23V2200/324A23V2200/30
Inventor 梁金钟秦琦肖雯娟林枫翔
Owner 哈尔滨美华生物技术股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap