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Oxidizing enzymes in the manufacture of paper materials

A technology of paper material and fatty acid oxidase, applied in the field of oxidase in the manufacture of paper material

Inactive Publication Date: 2005-02-02
NOVOZYMES AS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, none of the above-mentioned documents discloses the use of fatty acid oxidase as defined herein in the manufacture of paper materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Measuring the activity of fatty acid oxidase on linoleic acid

[0081] An "Oxi 3000 Oximeter" (WTW, Weilheim, Germany) containing a TriOxmatic 300 oxygen electrode and a standard reaction volume of 4 ml was used.

[0082] 10 mg of linoleic acid (10 ml of 60% linoleic acid) was dissolved in 1 ml of ethanol, then 2 microliters of Tween20 was added. From this raw matrix solution, 50 μl was added to a reaction beaker containing 3.85 ml of a buffer solution (Britton-Robinson: 100 mM phosphoric acid, acetic acid, and boric acid; pH was adjusted with NaOH) using a small stirring bar, stirred to mix the solution thoroughly, and The oxygen electrode is inserted into the reaction beaker. Add 100 microliters of purified enzyme solution, i.e. (a) lipoxygenase derived from Magnaporthe salvinii at a concentration of about 0.4 mg / ml; (b) lipoxygenase derived from Gaeumannomyces at a concentration of about 0.76 mg / ml (in It was approximately 0.02 mg / ml in the final reaction). The pr...

Embodiment 2

[0087] fatty acid oxidase

[0088] Four enzymes, two laccases and two lipoxygenases, were tested as shown below. Laccase derived from Polyporus pinsitus has a MW of 65 kDa by SDS-Page, 3.5 pl of IEF, and a temperature optimum of 60°C at pH 5.5. Laccase derived from Coprinus cinereus has a MW of 67-68 kDa by SDS-Page, 3.5-3.8 pl by IEF, and a temperature optimum of 65°C at pH 7.5. Enzymes were prepared and purified as described in WO96 / 00290 and US Patent 6,008,029. Two lipoxygenases were derived from Magnaporthe salvinii and Gaeumannomyces graminis and were prepared as described above.

[0089] The dosage of enzyme was adjusted to ensure that the maximum increase in absorbance per minute was obtained at 234nm / 530nm, ie in the range of 0.1-0.25 absorbance units / minute.

[0090] Matrix solution: 11.65 mg linoleic acid (60% Sigma), and 12.5 ml 0.56 mM syringaldazine (Sigma) in ethanol were mixed with deionized water and brought to a total volume of 25 ml.

[0091] Transfer ...

Embodiment 3

[0097] Pitch reduction with fatty acid oxidase

[0098] Asphalt samples were prepared as follows:

[0099] 50% Linoleic Acid 60% (Sigma L-1626),

[0100] 20% Abietic Acid (Sigma A9424),

[0101] 20% Oleic Acid (Merck 471),

[0102] 5% Cholesteryl Linoleate (Sigma C-0289),

[0103] 5% olive oil (Sigma O-1500),

[0104] Stir at 65°C for 30 minutes and store in the refrigerator for no more than 30 days.

[0105] To prepare a 0.1% asphalt suspension:

[0106] 50mg bitumen sample,

[0107] 1ml ethanol,

[0108] 1ml0.1MNaOH,

[0109] 48ml buffer (50mM borate pH9.0),

[0110] Stir at room temperature for 10 minutes.

[0111] The circular paper (diameter = 5.5-6mm; Multicopy80g / m 2 ) into wells of two 96-well microtiter plates (ID 269620 from NUNC) designated A and B. Two other similar microtiter plates C and D were also used, but without the discs. 100 microliters of the 0.1% bitumen suspension was added to each well of these four microtiter plates. The above-mentione...

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PUM

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Abstract

The use of fatty acid oxidizing enzymes in the manufacture of paper materials, such as paper, linerboard, corrugated paperboard, tissue, towels, corrugated containers and boxes. Examples of fatty acid oxidizing enzymes are oxygenases classified as EC 1.13.11. including any of the sub-classes thereof, such as lipoxygenase, EC 1.13.11.12. The effect of these enzymes is that the deposition of pitch is reduced, and bleaching and de-inking effects are also observed on the paper pulp and the resulting paper material. The fatty acid oxidizing enzyme can be used in combination with a substrate, with proteases, lipases, xylanases, cutinases, oxidoreductases, cellulases, endoglucanases amylases, mannanases, steryl esterases, and / or cholesterol esterases; or with surfactants and other adjuvants.

Description

field of invention [0001] The present invention relates to the use of fatty acid oxidase in the production of paper material and the method of producing paper material, the method comprises the step of treating papermaking pulp (papermaking pulp) and / or papermaking process water (process water) with fatty acid oxidase. Background of the invention [0002] The use of enzymes in the manufacture of paper materials is well known, and examples of enzymes used for this purpose include proteases, lipases, xylanases, amylases, cellulases, and various enzymes such as oxidoreductases (phenol oxidases). Oxidative enzymes such as laccases and peroxidases. [0003] The effects of these enzymes are general and can be used, for example, to control things such as pitch deposits, improving strength, deinking, improving drainage, softening tissue and bleaching, among others. [0004] In papermaking, dissolved and colloidal substances (DCS) are dispersed in process water during the production...

Claims

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Application Information

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IPC IPC(8): D21C9/00C12N9/02D21C5/00D21C5/02D21C9/08D21C9/10D21H21/02
CPCD21C9/08D21H21/02D21C9/10D21C5/027C12N9/0069C12Y113/11012D21C5/005Y02W30/64
Inventor 金·博尔奇尼尔·弗兰克斯亨里克·伦德徐辉罗菁
Owner NOVOZYMES AS
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