1 results about "Thromboxanes" patented technology

The application relates to an 11-dehydrothromboxane B2 detection kit and a detection method thereof, relates to the technical field of biological detection, and comprises an activation solution, an equilibrium solution, an elution solution, an elution solution, a reconstitution solution, an internal standard solution, a calibrator and a quality control product. The detection method comprises the following steps: S1, adjusting the pH of a urine sample to 2.5-3.0 by adding phosphoric acid; S2, sucking the urine sample after pH adjustment, the calibrator and the quality control product into a centrifugal tube, reacting at 37 DEG C for 2.5-3.5 hours, adding the internal standard solution, centrifuging, and taking the supernatant for solid-phase extraction treatment; S3, solid-phase extraction; and S4, calculating the concentration of 11-DH-TXB2 in the urine according to the liquid chromatogram and the peak area of 11-DH-TXB2 in the detection sample and the corresponding internal standard peak area. The application has reliable results and is convenient to calculate.