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L.plantarum UA149 strain and application thereof

A technology of Lactobacillus plantarum and UA149, applied in the direction of Lactobacillus, application, bacteria, etc., can solve the problems of liver damage, nervous system adverse reactions, restricted safe use, and many adverse reactions, and achieve hyperuricemia or gout prevention, The effect of reducing the level of thromboxane and alleviating the inflammatory response

Active Publication Date: 2019-07-26
JILIN MINGZHIYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these drugs have many adverse reactions. Benzbromarone can cause severe liver damage and adverse reactions to the nervous system; allopurinol can cause skin allergies, liver damage, and even life-threatening hypersensitivity allergic syndrome. To some extent, its safe use is limited

Method used

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  • L.plantarum UA149 strain and application thereof
  • L.plantarum UA149 strain and application thereof
  • L.plantarum UA149 strain and application thereof

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preparation example Construction

[0028] The preparation method of a kind of bacterial agent of the present invention comprises the following steps:

[0029] Plantarum UA149 strain was expanded to 2L, centrifuged at 6000r / min for 5min, resuspended in normal saline, centrifuged again at 6000r / min for 5min, resuspended in normal saline, washed, centrifuged again at 6000r / min for 5min, collected the sludge, dissolved in In 1L of freeze-drying protectant, freeze-dry to obtain Lactobacillus plantarum UA149 fermentation agent, the number of bacteria is 1.0×10 11 CFU / g.

[0030] Among them, the lyoprotectant is obtained by dissolving 40 g of glucose, 5 g of sodium ascorbate, 80 g of fructooligosaccharide, and 30 g of trehalose in tertiary water, and distilling the volume to 750 mL.

[0031] The Lactobacillus plantarum (L. plantarum) UA149 strain of the present invention can be used in the preparation of products with the function of reducing uric acid or in the preparation of products with the function of anti-gout....

Embodiment 1

[0046] The preparation of embodiment 1 plant Lactobacillus (L.plantarum) UA149 strain

[0047] 1. Isolation of Lactobacillus plantarum strains

[0048] 1. Sample material: succulent leaves.

[0049] Take 5g of succulent plant leaves, add 45mL of normal saline (10-fold dilution) and mix well, take 0.5mL of diluent, and dilute to 10 times with normal saline again. -3 , take 100μL liquid (10 -1 、10 -2 、10 -3 ) were spread on solid LAMVAB medium for cultivation (cultivation temperature 37°C, cultivation time 48-72h). Pick a single colony and inoculate it on a solid MRS medium plate to continue culturing (cultivation temperature 37°C, culture time 48h), and repeatedly streak culture and purify the solid MRS medium plate (cultivation temperature 37°C, culture time 48h), and obtain multiple strains Pure cultured strains.

[0050] 2. Inoculate the multiple strains of purely cultured strains obtained above into liquid MRS medium for culture (culture temperature 37°C, culture time...

Embodiment 2

[0075] Embodiment 2 Plant Lactobacillus (L.plantarum) UA149 strain of the present invention utilizes guanosine and inosine in vitro

[0076] 1. Establishment of HPLC standard curve

[0077] Take a certain amount of solution III, dilute it with solution I 2 times, 3 times, 4 times, 5 times, 6 times, 7 times respectively, shake well and pass through a 0.45 μm microporous membrane. Draw 20 μL each for injection analysis.

[0078] Chromatographic conditions:

[0079] HPLC chromatograph Agilent 1260LC, flow rate 1mL / min, column temperature 30°C, retention time 15min, measurement wavelength 254nm.

[0080] 2. Determination of nucleoside decomposition ability

[0081]The degradation process of guanosine and inosine was detected by high performance liquid chromatography, and the contents of inosine and guanosine were determined simultaneously. Inoculate the activated test strain with 3% inoculum into MRS liquid medium, culture for 17 hours, take 3 mL of culture solution at 4°C, ce...

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Abstract

The invention discloses an L.plantarum UA149 strain and an application thereof, and belongs to the field of functional food microorganisms. The L.plantarum UA149 strain is deposited in China Typical Culture Collection Center on November 29, 2018 with the preservation number of CCTCC No: M2018842. The strain can be apply to preparing products with a uric acid reducing function or a gout resisting function. Lactic acid bacteria separated and identified from the surface of fleshy plant leaves are taken as research objects, and a new strain of lactic acid bacteria is screened through a large number of experiments. Hyperuricemia model rats are established by potassium oxazinate combined with fructose water, continuous intragastric administration of the lactobacillus plantarum UA149 strain for 14 days can significantly reduce the level of uric acid; and during gout attack, the release of inflammatory factors thromboxane and leukotriene mediated by neutrophils is reduced, the influx of neutrophils into joints is avoided, and the symptoms of redness, swelling, pain, heat and the like are reduced.

Description

technical field [0001] The invention belongs to the technical field of functional food microorganisms, and in particular relates to a strain of Lactobacillus plantarum UA149 and an application thereof. Background technique [0002] Gout is a group of heterogeneous diseases caused by long-term purine metabolism disorder and elevated blood uric acid causing tissue damage. It is a serious metabolic disease that endangers human health. Gout can not only invade bones and joints, but also easily affect the kidneys and cardiovascular system, and induce obesity, hyperlipidemia, hypertension, diabetes, atherosclerosis and other diseases. [0003] In recent years, with the continuous improvement of people's living standards and changes in dietary structure, the intake of sugar, fat, and protein has increased significantly. The prevalence of gout has been increasing year by year in both European and American countries and Eastern countries. At present, the prevention of hyperuricemia ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P19/06A23L33/135C12R1/25
CPCA23L33/135A61P19/06A61K2035/115A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/30
Inventor 崔伟东梁铁
Owner JILIN MINGZHIYUAN BIOTECH
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