Chemiluminiscence detection method and chemiluminiscence detection kit for aspirin metabolism and platelet high reactivity

Abstract

The invention discloses a chemiluminiscence detection method and a chemiluminiscence detection kit for aspirin metabolism and platelet high reactivity. The kit comprises a monoclonal antibody combinedwith 11-dehydrothromboxane B2, a competitive product of 11-dehydrothromboxane B2, magnetic particles and a luminous substrate, wherein the monoclonal antibody is a mouse monoclonal antibody, the amino acid sequence of a light chain variable region of the mouse monoclonal antibody is shown as SEQ ID NO: 2 in a sequence table, and the amino acid sequence of a heavy chain variable region of the mouse monoclonal antibody is shown as SEQ ID NO: 4 in the sequence table. According to the present invention, with the application of the aspirin metabolism and platelet high-reactivity chemiluminiscencedetection method and the detection kit, whether the patient has aspirin drug resistance or resistance can be rapidly detected; the kit and the detection method provided by the invention have the advantages that the sensitivity is high, the accuracy is good, the kit and the detection method are not easily influenced by biochemical indexes, and the problem that the anti-platelet aggregation treatment of a clinical patient fails or the effect is poor due to aspirin resistance is solved.

Description

technical field [0001] The invention relates to a chemiluminescence detection method and a detection kit for aspirin metabolism and high platelet reactivity, belonging to the field of biological detection. Background technique [0002] 1. Metabolism of TXA2 and detection of 11dhTXB2 [0003] 11-dehydro-thromboxane B2 (11-dehydro-thromboxane B2, 11dhTXB2) is the terminal metabolite of thromboxane A2 (thromboxane A2, TXA2), which is mainly excreted by the kidneys, and is used in hemostasis and the occurrence of cardiovascular diseases. Play an important role. Prostaglandin H is produced from arachidonic acid by prostaglandin H synthase 1 and 2 (also known as COX-1 and COX-2). Prostaglandin H is chemically unstable and can be converted into a variety of biologically active prostaglandins under the action of isomerase, including TXA2, prostacyclin I2, prostaglandin D2, prostaglandin E2, and prostaglandin F2α. TXA2 is mainly synthesized in platelets through the COX-1 pathway. ...

AI Technical Summary

Problems solved by technology

However, the drug metabolism disorders or genetic defects that lead to true aspirin "resistance" have not been clarified, and the statement of AR is somewhat misleading. Therefore, high on-aspirin platelet reactivity (HAPR) under aspirin treatment is currently used to describe this disease. a state of incomplete response to aspirin

Although there are differences in urinary 11dhTXB2 levels in different populations, the inhibition rate after aspirin application is similar, mostly 60%-80%

Images