Compositions and methods for metabolic selection of transfected cells

A cell and cell sterol technology, which is applied in the field of compositions and methods for metabolic selection of transfected cells, can solve the problems of unstable coupling process, easy occurrence, and difficulty in filtering through small-pore sterile membranes.
CN101193918AActive Publication Date: 2008-06-04BIOFACTURA INC
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BIOFACTURA INC
Publication Date
2008-06-04

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Abstract

The present invention relates to novel selection marker vectors, and methods for using these vectors to generate stable gene expression systems in eukaryotic cells utilizing any enzyme useful in the eukaryotic sterol / cholesterol biosynthetic pathway, such as a 3-ketosteroid reductase, as a metabolic selection marker to select transfected cells. In one embodiment, the method comprises transfecting cells that are auxotrophic for cholesterol with a vector encoding 3-ketosteroid reductase and at least one heterologous protein, and selecting cells that have the ability to survive in medium lacking cholesterol and / or producing the heterologous protein in these cells in chemically defined and / or serum-free media.
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Description

[0001] Cross References to Related Applications

[0002] This application claims priority to US Provisional Application Serial No. 60 / 681,969 filed May 18, 2005 under 35.U.S.C. §119(e) of the US Patent Code, which is hereby incorporated by reference in its entirety. technical field

[0003] The present invention relates to novel marker selection vectors and methods for stably expressing gene products in eukaryotic cells using these vectors. Background technique

[0004] The development of in vitro culture technology for mammalian cells has revolutionized biological research. Cell culture technology can be used to evaluate the toxicity or effect of drugs in the early stage of development, and the use of human clinical trials in the early stage of drug development has high risks. Cell culture technology can also be used to generate complex human proteins such as monoclonal antibodies for therapeutic use, and can also be used as a platform for cell-based therapy. These cell c...

Claims

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