33 results about "Chromosome localisation" patented technology

The present invention provides parallel methods for determining nucleotide sequences and physical maps of polynucleotides associated with sample tags. This information can be used to determine the chromosomal locations of sample-tagged polynucleotides. In one embodiment, the polynucleotides are derived from genomic DNA coupled to insertion elements. As a result, the invention also provides parallel methods for locating the integration sites of insertion elements in the genome.

The invention discloses a chromosome automatic analysis method and a chromosome automatic analysis system based on depth learning, which can adopt multi-level processing, stratify the independent formand overlapping form of chromosomes, and perform cluster analysis on chromosome position coordinates, classification labels and classification confidence to output a karyotype map. In this manner, the method of chromosome segmentation based on depth learning can be adopted, independent of specific chromosomal morphological patterns, The method has high generalization ability, can adopt chromosomeclassification method based on depth learning, take into account the global morphology and banding characteristics of chromosomes, improve classification accuracy, can adopt multi-scale processing, more fully utilize the detected images, and effectively improve the segmentation effect in the case of chromosome overlap and adhesion.

The invention discloses a method for developing genome simple sequence repeats (SSR) molecular marker. The method comprises the steps of taking two plant samples, respectively establishing banks for DNA of the samples, thereby obtaining two corresponding libraries; respectively sequencing the libraries by an Illumina sequencing technology, then carrying out micropackaging, seeking in Contigs obtained through micropackage so as to obtain the SSR of the samples, and screening the common SSR in the two samples, wherein the common SSR has the same chromosomal location and the same repetitive unit; and carrying out polymorphism screening on the two samples according to the common SSR, and judging whether the two samples are in porlymorphism according to the difference of times of the repetition of the SSR of the two samples. The method not only meets the requirement of seeking a lot of SSR molecular markers, but also has low cost; due to the advantages of high polymorphism, dominant heredity, wide distribution and few template DNA, the SSR molecular markers can be widely used in genetic diversity analysis, genetic mapping, quantitative trait loci (QTL) location, molecular marker assisted breeding and the like.

The invention provides SSR molecular markers for identifying resistance of apple on the glomerella leaf spot. The molecular markers are S0607039, S0607001, S0506206, S0506078, S0506001, S0405195, S0405127, S0304673 and S0304011 respectively. The invention further provides primers used for multiplying the SSR molecular markers for identifying the resistance of apple on the glomerella leaf spot. The genes, resistant to glomerella leaf spot, of the apple are subjected to molecular marking through the self-designed SSR markers, so that the position of chromosomes and the genetic distance can be determined, and a tightly linked genetic map is established. The molecular marker closest to the resistance gene can be used for accurately identifying the resistance of apple on the glomerella leaf spot, so that an effective tool is provided for apple production practice and resistance breeding, and a good basis is laid for next cloning and functional verification of the resistance gene.

The invention relates to primers and a kit for detecting a molecular marker linked to major QTL for controlling corn stalk strength, a detection method for detecting corn stalk strength, and applications of the primers, the kit and the detection method, and belongs to the technical field of corn genetic breeding and molecular biology. According to the present invention, a recombinant selfing linepopulation containing 241 families is constructed through Zheng 58*D863F, and genetic linkage map analysis is performed, such that four QTL for controlling corn stalk strength are detected, wherein the high stalk strength major QTL qRPR1.07 is identified simultaneously at the position 1.07 of the chromosome 1 of the corn from Hainan Ledong and Henan Yuanyang Demonstration Base, and is positioned between two SSR markers bnlg1556 and umc2232 so as to explain nearly 20% of genetic variation; and the molecular marker linked to the major QTL for controlling corn stalk strength can directionally andprecisely screen corn materials with high stalk strength at the early stage of growth so as to save the breeding cost and the breeding time, and is used for lodging-resistant breeding of corn.

The present invention relates to a method for gene mapping from chromosome and phenotype data, which utilizes linkage disequilibrium between genetic markers m_i, which are polymorphic nucleic acid or protein sequences or strings of single-nucleotide polymorphisms deriving from a chromosomal region. The method according to the invention is based on discovering and assessing tree-like patterns in genetic marker data. It extracts, essentially in the form of substrings and prefix trees, information about the historical recombinations in the population. This infor-mation is used to locate fragments potentially inherited from a common diseased founder, and to map the disease gene into the most likely such fragment. The method measures for each chromosomal location the disequilibrium of the prefix tree of marker strings starting from the location, to assess the distribution of disease-associated chromosomes.

The invention belongs to the technical field of biology, and relates to a method for identifying and analyzing specific locus interaction proteins on the basis of a CRISPR/cas9 and peroxidase APEX2 system. The method comprises the following steps: plasmid transformation; biotin labeling of specific locus binding protein complex; enrichment of biotin-labeled protein complex by utilizing streptomycin magnetic beads; and assay and analysis of enriched proteins. An experimental analysis result shows that the method is applicable to the research and analysis of protein at any given chromosomal location, and meanwhile, the idea that the method combines the CRISPR gene editing system with APEX2 to carry out specific locus protein analysis is also applicable to the combination of other genome editing methods (such as TALEN) and the APEX2. The method has the advantages of simplicity in operation, high sensitivity, high specificity, good repeatability and adaption to the requirement of the research of local chromatin interaction at any given chromosomal location.

The invention discloses genetically engineered bacterium for production of beta-ionone and a construction method and application thereof. According to the invention, by using Yarrowia lipolytica as achassis cell, the beta-ionone expression module (about 14.5 kb) is firstly linearized and then transformed into Yarrowia lipolytica. Then the expression module is integrated into a designed chromosomal location mediated by a CRISPR/cas9 technology. The construction method is simple, fast and efficient. A large fragment integrated engineering strain can be obtained within 2-3 weeks, and the construction period of the engineering bacterium is obviously shortened. The beta-ionone initial output of shaking-flask fermentation of the genetically engineering bacterium obtained according to the methodof the invention can reach about 6.3 mg/L. According to the invention, a simple medium can be used for fermentation production of the perfume beta-ionone, one-time high-efficiency transformation andintegration of multiple genes can be realized, and the construction time of the engineering bacterium can be obviously shortened. The obtained engineering bacterium can be used to perform fermentationto produce the perfume beta-ionone by the use of simple carbon sources such as glucose, glycerin, etc., and has a good industrial application prospect.

Disclosed is a method for high-throughput detection of genome-wide modifications in a nucleic acid genome obtained from a cell or tissue caused by the activity of a designer nuclease comprising the following steps:

• • a) Extraction of the genomic DNA from cells that were exposed to a designer nuclease under conditions which allow the designer nuclease to introduce a DNA double-strand break (DSB) in the genomic DNA of the cell,
  • b) fragmentation of the nucleic acid to obtain random fragments,
  • c) performing an end repair in order to obtain blunt ends,
  • d) ligation with a linker comprising a sequence complementary to a so called “linker primer”,
  • e) performing a first nucleic acid amplification reaction with a “linker primer” and a so called “ON-target primer”, whereby one primer is located upstream and one primer is located downstream of the on-target site, wherein at least one decoy primer is present in the reaction mixture,
  • f) performing a second nucleic acid amplification reaction whereby so called “nested primers” are added to the reaction mixture, whereby one primer is complementary to the on-target locus and one primer complementary to the linker sequence,
  • g) performing a further nucleic acid amplification reaction whereby at least one code containing primers are added to the reaction mixture,
  • h) sequencing of the nested and barcoded amplification product, and
  • i) aligning the sequenced products with suitable bioinformatic means to a reference sequence to identify a chromosomal location that contains a genomic modification based on at least one DNA double strand break.

The present disclosure provides compositions and methods for acetylating histones at targeted chromosomal locations in a cell. In particular, the disclosure provides a fusion protein comprising a DNA binding domain and at least one histone acetyltransferase (HAT) domain, such that the DNA binding domain targets the fusion protein to a targeted chromosomal location and the HAT domain acetylates histones at the targeted location.

The invention provides a group of HLA-related SNP marks and a detection primer and a determination method thereof, and aims at providing a primer and a method for transplanting risk evaluation. The technical scheme is that the SNP marks comprise a first SNP mark, a second SNP mark and a third SNP mark, wherein the first SNP mark is a basic group A or T in the 32623514 position of the human No.6 chromosome; the second SNP marker is a basic group G or T in the 32623555 position of the human No.6 chromosome; the third SNP mark is a basic group A or T in the 32623560 position of the human No.6 chromosome; a primer pair for detecting the group of SNP markers in the claim 1 have the nucleotide sequences shown as SEQ ID NO:1 to 2 or SEQ ID NO:1 and 5 or SEQ ID NO:3 and 5 or SEQ ID NO:4 and 5. Thedetermination method of the HLA-related SNP sites comprises the following steps of (1) extracting the genome DNA of a host cell; (2) performing PCR amplification on the template DNA; purifying the PCR product SAP/Exon I; (3) respectively performing forward and/or reverse sequencing amplification on the purified PCR product by a sequencing primer; purifying the sequencing product; performing ABI 37030xl capillary electrophoresis sequencing so as to determine the SNP site and genotype.

The invention provides an HLA related SNP marker and a detection primer pair and a determination method thereof, and aims to provide a primer and method for evaluating transplantation risk. An SNP marker related with HLA is disclosed and is the base C or T on 32610275th site of human No.6 chromosome. A primer pair for detecting the SNP marker described by the claim one has nucleotide sequences represented by SEQ ID No1-2 or SEQ ID No3-4. A method of determining the SNP site comprises following steps: (1) extracting genome DNA of a host cell; (2) subjecting the template DNA to PCR amplification, and purifying the PCR product (SAP/Exon I); and (3) subjecting the purified PCR product to forward and/or backward sequencing amplification by using a sequencing primer, purifying the sequencing product, and carrying out ABI 3730x1 capillary electrophoresis sequencing to determine the SNP site and genotype thereof.

Versions of the invention are directed to methods (and related techniques) for a new type of association based linkage study technique using bi-allelic markers. The markers used in versions of the new linkage studies are chosen using a new two-dimensional concept of "closeness" in terms of marker distribution over a two-dimensional region having the orthogonal dimensions of chromosomal location and least common allele frequency. By using the two characteristics or two dimensions of marker chromosomal location and marker allele population frequency in this unique way, the power and systematic nature of genetic linkage studies using association based linkage tests is greatly increased. These unique two-dimensional linkage study techniques increase the power of association based linkage studies to localize trait causing genes or polymorphisms of modest effect such as human disease causing polymorphisms.