11 results about "Chromosome localisation" patented technology

The invention belongs to the technical field of crop molecular markers, and particularly discloses a molecular marker for identifying the salt tolerance of brassica napus in the germination period and application of the molecular marker. The molecular marker is located at the position of an A02 chromosome scafoldA028707654 of a brassica napus reference genome ZS11.v10, and the allelotype of the molecular marker is wild type C or mutant type CATATT insertion. The invention also provides a specific primer group for detecting the molecular marker, and the salt tolerance (sensitive type or tolerance type) of the brassica napus line can be quickly identified according to the existence of 411bp and 416bp bands through double PCR amplification and electrophoresis detection. The molecular marker is significantly associated with salt tolerance phenotypes, the detection method is rapid, accurate and low in cost, the molecular marker can be used for brassica napus salt tolerance germplasm resource screening and molecular marker-assisted breeding, and the breeding efficiency is significantly improved.

Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic / transgene junctions within SEQ ID NO: 2 or with genomic / transgene junctions as set forth in SEQ ID NO: 12 and / or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.

This application belongs to the field of molecular biological breeding and provides molecular markers for chicken weight-related structural variations and their applications. The molecular markers for chicken weight-related structural variations are: SV1 located at position 29007968 on chromosome 3, based on the chicken reference genome GRCg7b, with a reference allele of SEQ ID NO.1 and a substitute allele of A; or SV2 located at position 65895344 on chromosome 1, with a reference allele of G and a substitute allele of SEQ ID NO.2; SV3 located at position 1199450 on chromosome 12, with a reference allele of C and a substitute allele of SEQ ID NO.3; or SV4 located at position 37121335 on chromosome Z, with a reference allele of C and a substitute allele of SEQ ID NO.4. Experiments and verification have demonstrated that the above markers are significantly correlated with chicken weight, providing new molecular marker resources for the genetic improvement of broiler weight traits.

The application discloses a chromosome abnormality detection method, system and device, and relates to the technical field of medical image analysis. The method comprises the following steps: acquiring a chromosome patch sequence of a karyotype image; adopting a pre-trained convolutional neural network to extract local features of each patch in the chromosome patch sequence, converting the local features into an embedded vector sequence containing chromosome position semantics, inputting the embedded vector sequence into a multi-layer Transformer encoder after triple position coding to perform global correlation modeling, and outputting global features and patch features; performing chromosome position recognition based on the patch features to assist chromosome abnormality binary classification based on the global features, and outputting a probability distribution of the chromosome abnormality. The method significantly improves the missed detection rate of small-range structural abnormalities and the detection accuracy of global abnormalities such as translocation between chromosomes.

The invention discloses an SNP molecular marker located on a pig chromosome 4 and related to the thickness of waist meat. The SNP site of the SNP molecular marker corresponds to Ggt at the position of 16572687bp of a chromosome 4 of a reference sequence of an international pig genome version 11.1; a mutation; the nucleotide sequence of the SNP molecular marker is SEQ ID NO.1, wherein M in the sequence is G or A. Researches show that the polymorphism of the basic group at the site leads to the difference of the thickness of the waist meat. Accordingly, the inventor establishes a corresponding pig genetic improvement method, and by optimizing the dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the thickness of pig waist meat can be increased, and the progress of pig genetic improvement can be accelerated, so that the economic benefit of breeding pig breeding can be effectively improved. Besides, the inventor also researches and designs a primer pair for amplifying the nucleotide sequence of the SNP molecular marker, and the carcass traits can be quickly and accurately bred through the primer pair, so that the breeding process is accelerated.

The invention provides a fusion gene rapid screening method and system for RNA sequencing data, and the method comprises the steps: receiving a tab format comparison result file outputted by a sequence comparison tool, and screening comparison fragments in the comparison result file according to a preset similarity threshold value and a coverage rate threshold value; in the comparison fragments, different mapping fragments of the same sequencing are combined and compared, and the overlapping degree and the total coverage degree of fragment combinations on sequencing are calculated; judging whether the event is a fragment fusion event at least according to the chromosome position, the fragment spacing, the fragment combination overlapping degree and the fragment combination total coverage degree; and if the event is a fusion event, outputting fusion gene information. Through the scheme, the fusion gene detection efficiency can be improved, and the accuracy of a detection result is guaranteed.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for influencing chicken laying age in days by influencing ABCB5 gene expression and application of the SNP molecular marker, and belongs to the fields of molecular genetics and agricultural animal breeding, the SNP site is located at the 29957558bp position of a chicken chromosome 2, the reference genome version is GRCg7b, the SNP molecular marker has T / C polymorphism, and the SNP molecular marker can be used for identifying chicken laying age in days. The individual with the genotype CC at the position has earlier laying day age than other genotypes. The invention identifies a molecular genetic marker system which is significantly related to ABCB5 gene expression and can be used for predicting and selecting the day age at the beginning of laying of chickens, and provides a new technical path and theoretical support for molecular breeding of prematurity traits of laying hens.

The invention belongs to the technical field of molecular biology, and particularly relates to a molecular marker related to the number of lateral roots of medicago sativa and application of the molecular marker. The invention aims to shorten the period of breeding alfalfa materials with excellent root systems by finding proper molecular markers. The technical scheme of the invention is a molecular marker related to the number of lateral roots of medicago sativa. The specific information is as follows: chromosome: Chr5; the position is 28984960; sNP (Single Nucleotide Polymorphism) typing: C / G. A KASP typing primer is designed aiming at the site. According to the designed KASP typing primer, a large number of materials are detected, and the result shows that the numbers of lateral roots of the materials with different genotypes are remarkably different. By adopting the molecular marker and the KASP typing primer disclosed by the invention, genotype screening can be completed in a seedling stage, and selection can be carried out without waiting for ripening of plants.

The invention belongs to the technical field of molecular biology, and particularly relates to a molecular marker related to the stalk diameter under salt stress of medicago sativa and application of the molecular marker. The invention aims to solve the technical problems that the breeding of an alfalfa variety with ideal stem diameter character under salt stress condition mainly depends on phenotypic determination for many years, the breeding process is time-consuming and labor-consuming, is greatly influenced by the environment, and is insufficient in selection efficiency and accuracy. The technical scheme of the invention is a molecular marker related to the diameter of a stalk under salt stress of medicago sativa. The locus information of the molecular marker is as follows: chromosome: chr5; the position is 58795369; sNP (Single Nucleotide Polymorphism) typing: G / T. The invention also provides a primer group for amplifying the molecular marker. The method can be used for early prediction of the stalk diameter character and molecular marker assisted breeding under the condition of salt stress of medicago sativa.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for regulating and controlling the length of a corn kernel and application of the SNP molecular marker, and belongs to the technical field of molecular genetic breeding. Specifically, on the basis of whole genome association analysis (GWAS), an SNP site (C / A variation) remarkably related to the grain length is identified at the positions of 135bp, 186bp and 086bp of a chromosome 8 of a corn B73RefGenv5 genome, and the grain length can be remarkably increased by a C allele. The sequence of a KASP primer for genotyping of the site is as shown in SEQ ID NO: 1-3. By utilizing the SNP molecular marker and the corresponding KASP primer group provided by the invention, high-throughput and high-accuracy genotype identification can be realized, and the SNP molecular marker has the advantages of high stability, high throughput, low cost and the like, can be used for early auxiliary selection, germplasm resource screening and molecular design breeding of corn kernel length related traits, and provides an effective tool for genetic improvement of corn yield traits.

The present application belongs to the technical field of molecular biology, and particularly relates to a molecular marker related to the number of lateral roots of Medicago sativa and application thereof. The purpose of the present application is to shorten the period of breeding alfalfa materials with excellent root system by finding a suitable molecular marker. The technical scheme of the present application is a molecular marker related to the number of lateral roots of Medicago sativa, and the specific information is as follows: chromosome: Chr5; position: 28984960; SNP typing: C / G. A KASP typing primer is designed for the site. A large number of materials are detected according to the designed KASP typing primer, and the results show that the number of lateral roots of different genotypes of materials has significant difference. The molecular marker and the KASP typing primer of the present application can complete the genotype screening at the seedling stage, and the selection can be performed without waiting for the plants to mature.