The use of snp markers in the preparation of kits for assisting in assessing the risk of transplant rejection

Abstract

The invention provides a group of HLA-related SNP marks and a detection primer and a determination method thereof, and aims at providing a primer and a method for transplanting risk evaluation. The technical scheme is that the SNP marks comprise a first SNP mark, a second SNP mark and a third SNP mark, wherein the first SNP mark is a basic group A or T in the 32623514 position of the human No.6 chromosome; the second SNP marker is a basic group G or T in the 32623555 position of the human No.6 chromosome; the third SNP mark is a basic group A or T in the 32623560 position of the human No.6 chromosome; a primer pair for detecting the group of SNP markers in the claim 1 have the nucleotide sequences shown as SEQ ID NO:1 to 2 or SEQ ID NO:1 and 5 or SEQ ID NO:3 and 5 or SEQ ID NO:4 and 5. Thedetermination method of the HLA-related SNP sites comprises the following steps of (1) extracting the genome DNA of a host cell; (2) performing PCR amplification on the template DNA; purifying the PCR product SAP / Exon I; (3) respectively performing forward and / or reverse sequencing amplification on the purified PCR product by a sequencing primer; purifying the sequencing product; performing ABI 37030xl capillary electrophoresis sequencing so as to determine the SNP site and genotype.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to an analysis of SNP markers related to the HLA DQ promoter, and prediction of rejection risk assessment after organ transplantation in patients through the group of SNP markers. Background technique [0002] In the field of organ transplantation, various rejection reactions caused by HLA antibody-mediated humoral immunity are the most critical factors affecting the survival of human / graft after transplantation. [0003] SNP (Single nucleotide polymorphism, single nucleotide polymorphism) mainly refers to the DNA sequence polymorphism caused by single nucleotide variation at the genome level. [0004] In the human population, HLA (Human leukocyte antigen, human leukocyte antigen) has abundant polymorphisms. In organ transplantation, HLA antigens in the endothelial cells of donor organs are often used as recognition antigens, causing a series of rejection reactions and affecting th...

AI Technical Summary

Problems solved by technology

[0006] However, there is no clinical report on the application of DQ promoter SNP analysis to organ transplantation. If the DQ promoter SNP can be analyzed to clarify the expression level of DQ protein in patients under immune status, it can be predicted by the patient's antigen presentation ability. The risk of humoral rejection after surgery, promote the status quo of early prediction of organ transplant rejection in my country, and guide the selection of donors in clinical practice, the dosage of immunosuppressants, etc.

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