A method for detecting the position of large segment repeats in Saccharomyces cerevisiae chromosome

Abstract

The invention relates to the field of molecular biology, in particular to a method for detecting a klenow fragment repetition position in a saccharomyces cerevisiae chromosome.By combining genomic sequencing with endoreduplication backcrossing, the position of repeated klenow fragment genome DNA in the chromosome is confirmed quickly.Through the endoreduplication backcrossing method, the position of the repeated klenow fragment genome DNA in the saccharomyces cerevisiae chromosome can be detected efficiently and quickly and repaired, repeated klenow fragment genome DNA is deleted, there is no need to precisely confirm the position of a klenow fragment repetition site or conduct cumbersome experimental data analysis on saccharomyces cerevisiae to be detected, operation is easy, and the experimental period is short.

Description

technical field

[0001] The invention relates to the field of molecular biology, in particular to a method for detecting the repeat position of a large segment of yeast chromosome. Background technique

[0002] Saccharomyces cerevisiae, also known as baker's yeast or budding yeast. Saccharomyces cerevisiae is the yeast most widely related to humans, not only because it is traditionally used to make food such as bread and steamed bread and brew wine, but also used as a eukaryotic model organism in modern molecular and cell biology, and its role is equivalent to that of prokaryotic The model organism Escherichia coli. Yeast cells have high-efficiency homologous recombination characteristics (Ma, H., et al. Gene (1987), 58, 201-216), so they are widely used in the research of plasmid construction, gene knockout and even genome construction (Gibson, D.G., et al. Proc. Natl. Acad. Sci. U.S.A (2008). 105, 20404-20409). The researchers of the artificially synthesized Saccharomyce...

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