SSR Molecular Marker and Its Application for Identifying Resistance and Susceptibility to Anthracnose Leaf Blight of Apple
A molecular marker, anthracnose technology, applied in the field of molecular biology, to speed up discovery and research on disease resistance mechanisms, increase reliability, and speed up the pace
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[0025] The present invention will be further described below in conjunction with specific examples. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.
[0026] 1 Materials and methods
[0027] 1.1 Test material
[0028] 1.1.1 Test materials
[0029] The F1 populations and 4 parents of 4 hybrid combinations planted in the apple test base of Qingdao Agricultural University (Jiaozhou City, Shandong Province) from 2009 to 2014 were selected as test materials for indoor artificial inoculation identification. The 4 populations are: 207 F1 plants of 'Jinguan'×'Fuji', 95 F1 plants of 'Fuji'×'Jinguan', 262 F1 plants of 'Gala'×'Fuji', 'Fuji'×'QF- There were 198 strains in the 2'F1 generation.
[0030] Among them, the group 'Jinguan'×'Fuji' was also used for molecular marker research of apple anthracno...
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