Genetically engineered bacterium for production of beta-ionone and construction method and application thereof

A technology of genetically engineered bacteria and ionone, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problem of less bio-heterogeneous synthesis of β-ionone, and achieve good industrial application prospects , good application prospects, and the effect of shortening the construction cycle
CN108949599AInactive Publication Date: 2018-12-07SOUTH CHINA UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTH CHINA UNIV OF TECH
Publication Date
2018-12-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses genetically engineered bacterium for production of beta-ionone and a construction method and application thereof. According to the invention, by using Yarrowia lipolytica as achassis cell, the beta-ionone expression module (about 14.5 kb) is firstly linearized and then transformed into Yarrowia lipolytica. Then the expression module is integrated into a designed chromosomal location mediated by a CRISPR / cas9 technology. The construction method is simple, fast and efficient. A large fragment integrated engineering strain can be obtained within 2-3 weeks, and the construction period of the engineering bacterium is obviously shortened. The beta-ionone initial output of shaking-flask fermentation of the genetically engineering bacterium obtained according to the methodof the invention can reach about 6.3 mg / L. According to the invention, a simple medium can be used for fermentation production of the perfume beta-ionone, one-time high-efficiency transformation andintegration of multiple genes can be realized, and the construction time of the engineering bacterium can be obviously shortened. The obtained engineering bacterium can be used to perform fermentationto produce the perfume beta-ionone by the use of simple carbon sources such as glucose, glycerin, etc., and has a good industrial application prospect.
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Description

technical field

[0001] The invention belongs to the field of microbial fermentation, and in particular relates to a genetically engineered bacterium producing β-ionone and its construction method and application. Background technique

[0002] Ionone is a high-grade spice and an indispensable and important original spice in perfumery. It has a wide range of uses and is in great demand. The molecular formula of ionone is C 13 h 20 O, according to the position of the double bond, ionone can be divided into three isomers of α-body, β-body and γ-body, and the mixed form of α-body and β-body exists in nature , where β-ionone is used as flavoring agent and flavoring agent for high-grade fine daily cosmetics, and is also an important raw material for the synthesis of vitamin A in the pharmaceutical industry. In the food industry, ionone is often used as a flavor enhancer for high-end food and beverages. It is an edible flavor allowed by China's GB2760-2011 regulations. It is main...

Claims

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