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Molecular biology method for identifying gender of chub fry

A technique for identification and silver carp, applied in the field of molecular biology, can solve problems such as judging gender characteristics

Inactive Publication Date: 2009-12-30
HENAN NORMAL UNIV
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Problems solved by technology

However, since the first sexual maturity of silver carp takes 3 to 4 years, its gender characteristics cannot be judged by its appearance at the seedling stage. Therefore, how to identify the genetic sex quickly and easily from the seedling population has become a constraint to the successful breeding of silver carp. prerequisites

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  • Molecular biology method for identifying gender of chub fry
  • Molecular biology method for identifying gender of chub fry
  • Molecular biology method for identifying gender of chub fry

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Embodiment Construction

[0009] Obtaining of male-specific primers and verification of sex-specificity

[0010] After gonad anatomy and sex identification, the genomic DNA of male and female individuals was extracted, and 220 random primers were used for RAPD-PCR amplification. When random primer S2107 was used, a stable band was amplified in male individuals, and the band was amplified in female individuals. It does not exist in the individual, and a male-specific fragment of about 800bp was obtained by cloning and sequencing, named Hmmf (its DNA sequence is shown in Table 1). This fragment is a non-coding sequence. The sequence was compared on Genebank and found no sequences that are homologous to it. This band is the male-specific band of silver carp. After sequencing, a pair of male-specific primers Hmmf1 and Hmmf2 were designed, and the GAPDH housekeeping gene of silver carp was searched by NCBI, and a pair of primers Gapdh1 and Gapdh2 (its DNA The sequence is shown in Table 2). Using the desig...

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Abstract

The invention discloses a molecular biology method for identifying gender of a chub fry, belonging to the field of molecular biology, and aiming at providing a specific segment of chub fry DNA gender, a specific primer of male and the method for identifying gender of the chub fry. The technical proposal is that the sequence of the specific segment is Hmmf1 and Hmmf2. The molecular biology method for identifying gender of the chub fry is to use the designed specific primer and a contrast housekeeping gene GAPDH primer to carry out PCR amplification with chub individual gene group DNA as a template, the reaction system is 25 mu l, PCR amplification is carried out on a PCR instrument according to 30 circulating parameters, a gel imaging system is used for observing and shooting for record, 100bp DNA ladder is used as a molecular marker, the appeared chub fry with a band of 800bp mesh is male individual, the chub fry without band is female individual. The invention is used for identification of gender of the chub fry.

Description

Technical field: [0001] The invention relates to molecular biology, in particular to a molecular biological method for identifying the sex of silver carp seedlings. Background technique: [0002] my country is rich in fish resources, many of which have sex differences in growth rate. Therefore, research on sex-determining genes and sex-specific markers of these fish has high theoretical significance for fish sex control. Silver carp (Hypophthalmichthys molitrix) is also called silver carp, water carp, jumping carp, silver carp, belongs to Cyprinidae, Cyprinidae, and is one of the four famous big fishes. The well-known specialty economic fish has a long history of breeding. It is cultivated in ponds, lakes and reservoirs in my country, and its aquaculture output has always ranked first in freshwater aquaculture in my country. Silver carp females grow fast and grow rapidly. At present, the combination of artificial gynogenesis and hormone sex reversal to create an all-female...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 常重杰陈建军杜启艳夏晓华王友利
Owner HENAN NORMAL UNIV
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