Fluorescence labeling composite amplification kit for detecting human Y chromosome rapid mutation STR loci and application thereof

A technology of multiple amplification and fluorescent labeling, applied in the field of molecular genetics, can solve the problems of inability to effectively distinguish male individuals of the same paternal line, low application value of Y chromosome STR typing, poor Y chromosome gene polymorphism, etc., and achieve male individual identification High capacity, improved space utilization, high amplification specificity and high amplification sensitivity

Active Publication Date: 2019-06-25
GUANGDONG HUAMEI ZHONGYUAN BIOLOGICAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]The male attribute and paternal inheritance mode of the existing low-mutation Y chromosome STR make it useful in forensic sex crime investigation, paternal relationship identification and on-site biological sample population source Inference and other aspects have unique application value, bu

Method used

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  • Fluorescence labeling composite amplification kit for detecting human Y chromosome rapid mutation STR loci and application thereof
  • Fluorescence labeling composite amplification kit for detecting human Y chromosome rapid mutation STR loci and application thereof
  • Fluorescence labeling composite amplification kit for detecting human Y chromosome rapid mutation STR loci and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Screening of Y chromosome rapid mutation STR locus

[0029] Through the research on the polymorphism and mutation rate of Y chromosome STR loci, DYF387S1a / b, DYF399S1a / b / c, DYF403S1a1 / a2 / a3 / b, DYF404S1a / b, DYS449, DYS508, DYS516, DYS518, DYS526a were finally screened out / b, DYS534, DYS547, DYS565, DYS570, DYS573, DYS576, DYS612, DYS626, DYS627 and DYS640, a total of 27 Y chromosome rapid mutation STR loci. The information of each gene locus is shown in the table below:

[0030] Table 2. Y chromosome rapid mutation STR locus information

[0031]

Embodiment 2

[0032] Example 2. Y chromosome rapid mutation STR locus arrangement

[0033] Based on the above 27 loci, a unique locus arrangement and chemical fluorescent dye labeling method were designed. The 27 loci were labeled with four different fluorescent dyes, and were divided into four groups according to the different fluorescent dyes labeled. As follows: the first group is DYS526a / b, DYS570, DYF387S1a / b, DYS516, the fluorescent dye is 6-FAM; the second group is DYS576, DYF403S1a1 / a2 / a3 / b, DYS640, DYS547, the fluorescent dye is HEX; The group is DYS508, DYS612, DYS573, DYF399S1a / b / c, DYS627, DYS518, and the fluorescent dye is TAMRA; the fourth group is DYS565, DYF404S1a / b, DYS626, DYS449, DYS534, and the fluorescent dye is ROX. The gene locus layout is as follows figure 1 shown.

Embodiment 3

[0034] Example 3. Kit components and amplification system composition

[0035] The kit components and amplification system composition of the present invention are specifically as shown in Table 3 and Table 4:

[0036] Table 3. Components of the kit

[0037]

[0038] Table 4. Composition of the amplification system

[0039]

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Abstract

The invention provides a fluorescence labeling composite amplification kit for detecting 27 human Y chromosome rapid mutation STR loci. 27 STR loci on the Y chromosome with high mutation rate can be amplified multiply in a single reaction. The 27 Y chromosome fast mutant STR loci are divided into four groups by designing unique locus arrangement and specific primers and labeled with different fluorescent dyes. A composite amplification system comprises all the Y chromosome STR loci with high mutation rate and high polymorphism, the male individual recognition capability is high, and the kit can be used as an effective supplement for a conventional Y chromosome STR kit in family segmentation and case investigation. The fluorescence labeling composite amplification system has good primer specificity, strong sample adaptability, simple method operation, short detection time and high efficiency.

Description

technical field [0001] The invention relates to the field of molecular genetics, in particular to a fluorescent labeling compound amplification kit for detecting 27 rapidly mutated STR loci of human Y chromosome and its application, which can be applied to paternity identification, database construction and investigation, DNA family tree construction, etc. aspect. Background technique [0002] In recent years, forensic DNA typing technology has developed rapidly. At present, it has been able to carry out DNA typing on a variety of test materials, reaching the level of identification, and has become one of the methods with the highest technological content and the strongest practicability in criminal technology. The technical level is basically the same as Synchronized around the world, the test results have been widely accepted by judicial organs, especially judicial organs, and DNA analysis technology has become one of the most important basis for criminal investigation and...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888
CPCY02A90/10
Inventor 刘超杜蔚安郑文彦刘宏周咏松徐曲毅王邦超刘长晖
Owner GUANGDONG HUAMEI ZHONGYUAN BIOLOGICAL SCI & TECH
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