Fluorescence labeling composite amplification kit for detecting human Y chromosome rapid mutation STR loci and application thereof

Abstract

The invention provides a fluorescence labeling composite amplification kit for detecting 27 human Y chromosome rapid mutation STR loci. 27 STR loci on the Y chromosome with high mutation rate can be amplified multiply in a single reaction. The 27 Y chromosome fast mutant STR loci are divided into four groups by designing unique locus arrangement and specific primers and labeled with different fluorescent dyes. A composite amplification system comprises all the Y chromosome STR loci with high mutation rate and high polymorphism, the male individual recognition capability is high, and the kit can be used as an effective supplement for a conventional Y chromosome STR kit in family segmentation and case investigation. The fluorescence labeling composite amplification system has good primer specificity, strong sample adaptability, simple method operation, short detection time and high efficiency.

Description

technical field [0001] The invention relates to the field of molecular genetics, in particular to a fluorescent labeling compound amplification kit for detecting 27 rapidly mutated STR loci of human Y chromosome and its application, which can be applied to paternity identification, database construction and investigation, DNA family tree construction, etc. aspect. Background technique [0002] In recent years, forensic DNA typing technology has developed rapidly. At present, it has been able to carry out DNA typing on a variety of test materials, reaching the level of identification, and has become one of the methods with the highest technological content and the strongest practicability in criminal technology. The technical level is basically the same as Synchronized around the world, the test results have been widely accepted by judicial organs, especially judicial organs, and DNA analysis technology has become one of the most important basis for criminal investigation and...

AI Technical Summary

Problems solved by technology

[0004]The male attribute and paternal inheritance mode of the existing low-mutation Y chromosome STR make it useful in forensic sex crime investigation, paternal relationship identification and on-site biological sample population source Inference and other aspects have unique application value, but there is also the limitation that male individuals of the same paternal line cannot be effectively distinguished

In addition, some groups that are relatively closed or have experienced bottleneck effects have low application value of Y chromosome STR typing due to poor Y chromosome gene polymorphism

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