Rapid identification method for half-smooth tongue-sole genetic sex

A kind of semi-smooth tongue sole, identification method technology, applied in biochemical equipment and methods, microbial determination/inspection and other directions, can solve problems such as unreported, achieve the effects of improving breeding output, improving economic benefits, and major application value

Inactive Publication Date: 2008-08-13
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports about the rapid identification method of fish genetic sex, especially the rapid identification method of fish genetic sex using the LAMP principle.

Method used

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  • Rapid identification method for half-smooth tongue-sole genetic sex
  • Rapid identification method for half-smooth tongue-sole genetic sex
  • Rapid identification method for half-smooth tongue-sole genetic sex

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Embodiment Construction

[0059] The present invention will be further elaborated below in conjunction with the accompanying drawings and embodiments.

[0060] Figure 1 shows that six specific primers were designed according to the female-specific marker sequence of half-smooth tongue sole, and the specific sequence and the position of the primers are indicated in Figure 1.

[0061] The serial number on the left side in Figure 1 indicates the sequence position, and B1, B2, B3, LP1, LP2, F1, F2, and F3 respectively indicate the 8 sites identified by the 6 primers used. The 6 primer sequences used are as follows:

[0062] Primer BIP: 5-GTCCTAAGTAAACTGAACCTGGAGTCTTTTACATCAACGTTCTTATATGCCAACAG-3

[0063] Primer FIP: 5-GAGCAACATCTTATCGCCTCAAGTTTTTAACCCACTGTGTCACCTGAGA-3

[0064] Primer B3: 5-AAGTTAGGCAGTTCGCTGAG-3

[0065] Primer F3: 5-CACCATCATTGTAAAACTAGTCTCG-3

[0066] Primer LP1: 5-AACAAATATGACACATAATTCTTGGCTTCT-3

[0067] Primer LP2: 5-CCAGGGCTGGAGAAAGCAG-3

[0068] Wherein, the primer BIP is form...

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Abstract

The invention is a method for rapid identifying genetic sex of tongue sole, comprising rapid extracting DNA of tongue sole, designing and synthesizing of LAMP specific primer, establishing a LAMP reaction system, detecting of LAMP reaction products and identifying genetic sex. By female specific molecular marker sequence of tongue sole, the invention designs specific primer, performs transitory nucleic acid amplification under constant temperature, the genetic female individual produces specific DNA fragment, while the male individual does not produce such DNA amplification product. The invention creates a LAMP method for rapid detecting genetic sex of tongue sole for the first time, can be used in culture farm, and the testing operation can be finished within 2.5h. The invention has the characteristic of being advanced, rapid, specific, precise, sensitive, simple and in no need of large-scale instruments, thus providing a new technological approach for fish sex detection and control. The invention has important application value in control of tongue sole sex and production of unisex fry, and wide application prospect in identification of genetic sex of other fish species and research of sex control.

Description

technical field [0001] The invention belongs to fish genetic sex identification technology in the technical field of aquatic organisms, and in particular relates to a method for rapid identification of genetic sex of tongue sole. Molecular biology methods for application in fish sex control and parthenogenesis. Background technique [0002] Cynoglossus semiliaevis (Cynoglossus semiliaevis) is a rare and valuable seawater fish endemic to my country. It belongs to warm-water demersal fish in coastal waters. It is distributed in coastal areas of my country, mostly in the Yellow Sea and Bohai Sea. Semi-smooth tongue sole is popular with consumers because of its delicious taste, tender meat and rich nutrition. Its market value is extremely high and its breeding prospect is very broad. In the past two years, breakthroughs have been made in the artificial breeding technology of tongue sole, with an annual output of 3 to 5 million seedlings of tongue sole. Studies have shown that t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陈松林徐建勇
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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