Establishing method for fish male sterile model

Abstract

The invention discloses an establishing method for a fish male sterile model. A CRISPR / Cas9 technique is utilized to knock out a meiosis-related gene spo11; heterozygote is fertile; male individuals in a homozygote colony acquired through selfing of heterozygote are sterile, so that the fish male sterile model is established. The sterile male individuals can be specifically acquired according to the method disclosed by the invention; the male sterile individuals can be continuously supplied in the manner of establishing a knockout family; the operation is simple and the time is saved.

Description

technical field [0001] The invention relates to a method for establishing a fish male sterility model, belonging to the field of molecular biology. Background technique [0002] Fish obtain energy from the outside world, part of which is used for growth and part for reproduction. During the reproductive period, growth slows down or even stops, and the reproductive period of many fish is between the growth periods. Because all-male fish grows faster and the individuals are relatively large, they become the object of everyone's competition for breeding. Therefore, in recent years, how to establish male sterile lines in fish has become one of the more popular research directions. Spo11 is a homologous protein of type II topoisomerase, which is involved in the formation of DNA double-strand break complexes (DSBs) and plays an important role in the occurrence of homologous recombination in meiosis. Recombination plays a crucial role in meiosis in most sexually reproducing organi...

AI Technical Summary

Problems solved by technology

In many years of experiments, the inventor found that knocking out a meiosis-related gene spo11 led to male-specific sterility in zebrafish, while females were almost unaffected, but no one has reported that the gene spo11 has this function

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