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Method and system for acquiring age of male individual in Chinese population

A male and individual technology, applied in the direction of biochemical equipment and methods, microbiological measurement/inspection, etc., can solve the problem of male individual absence, etc., and achieve the effect of narrowing the search scope, improving the efficiency of solving cases, and good inference accuracy

Active Publication Date: 2019-04-09
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the studies are on European and American populations, and there are few studies on Chinese populations, especially male individuals in Chinese populations.

Method used

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  • Method and system for acquiring age of male individual in Chinese population
  • Method and system for acquiring age of male individual in Chinese population
  • Method and system for acquiring age of male individual in Chinese population

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Embodiment 1 Using the method and system provided by the present invention to obtain the age of male individuals in the Chinese population

[0054] Hereinafter, a male individual is taken as an example to illustrate the implementation process of the solution of the present invention.

[0055] 1. Extract the DNA of the individual to be tested as a template

[0056] The DNA solution of the above-mentioned one male individual was extracted separately using a DNA blood midi kit (QIAGEN, Hilden, Germany).

[0057] 2. Perform PCR amplification reaction on the extracted DNA template

[0058] 2.1. Primer pool configuration

[0059] The configuration of the amplification primer pool, wherein the amplification primer set is 9 pairs of amplification primers corresponding to the 9 CpG sites, each pair of amplification primers can amplify the DNA to be detected including its corresponding CpG site; in this embodiment, preferably, the amplification primer set of the 9 CpG sites is...

Embodiment 2

[0083] Using the same method and system as in Example 1, the ages of all 65 unrelated Chinese male individuals of different ages were estimated using the same steps. To test the accuracy of the above system, the results are shown in the following table:

[0084]

[0085] The age error range of male individuals obtained by the method of this embodiment is about 2-5 years old, indicating that the method and system of the present invention can be used to estimate the age of individuals aged 15 to 75, and has good accuracy, and can provide data for case detection support.

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Abstract

The invention provides a method and a system for acquiring the age of a male individual in a Chinese population. The method comprises the following steps: extracting DNA of the male individual; acquiring the methylation rates of 9 CpG sites of the DNA; and according to the methylation rates of the respective CpG loci, performing regression analysis on the 9 CpG loci and the age by R software to construct a regression model so as to acquire the age of the male individual in the Chinese population. According to a scheme provided by the invention, the age of the male individual in the Chinese population can be accurately acquired, and the age of the individual can be inferred from biodetection materials, such as blood or a blood mark sample, which are left on a crime scene in an actual publicsecurity combat, so that quick locking of a criminal suspect scope is facilitated, a clue is provided for case detection and the speed of the case detection is increased.

Description

technical field [0001] The present invention relates to a method and a system for obtaining the age of a Chinese population, in particular to a method and a system for obtaining the age of a male individual in a Chinese population. Background technique [0002] The estimation of individual age has always been one of the important research topics in forensic science. Currently, the assessment of individual age is mainly based on the temporal changes of the physical and chemical properties of tissues such as bones and teeth and various substances in the tissues with age. Due to the extremely complex physiological changes and influencing factors of the growth and development of various tissues and organs in organisms, different methods for inferring age have their application value, but they all have certain limitations. For example, 1) The bone age estimation of the six major joints used in the research of the Physical Evidence Center of the Ministry of Public Security is appl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/154
Inventor 丰蕾赵蕾刘凡李姗飞彭付端季安全
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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