Fluorescence labeling composite amplification kit for human Y chromosome 27 STR gene loci

Abstract

The invention discloses a fluorescence labeling composite amplification kit for human Y chromosome 27 STR gene loci, which can simultaneously amplify 27 Y chromosome STR gene loci (including 24 low-mutation-rate Y chromosome STR gene loci and 3 high-mutation-rate Y chromosome STR gene loci) in a single multiplex reaction. By designing the unique gene locus configuration mode and specific primer sequences, the 27 gene loci are divided into 4 groups which are used for different fluorescence labelings. The composite amplification system has the advantages of wide temperature resistance range, high adaptability of the detection material, and high identification capacity for irrelevant and close relative male individuals.

Description

technical field [0001] The invention relates to a detection kit for male individual identification, in particular to a fluorescent labeling composite amplification kit for 27 STR loci of human Y chromosome, which can be applied to forensic individual identification, paternity identification, DNA family tree construction and the like. Background technique [0002] STR genetic markers, also known as microsatellite DNA, widely exist in prokaryotic and eukaryotic genomes, and are a type of repeating sequence consisting of 2 to 6 nucleotides as a repeating unit up to dozens of nucleotides. Different numbers of core sequences were arranged in tandem repeats, which showed length polymorphism. According to the conserved sequences at both ends, primers were designed, PCR was performed, and polymorphisms of STR genetic markers were detected by polyacrylamide, agarose gel electrophoresis, capillary electrophoresis, etc. [0003] The Y chromosome STR genetic marker refers to the short ...

AI Technical Summary

Problems solved by technology

In order to improve the ability of individual recognition, it is necessary to continuously add new Y chromosome STR genetic markers to form a high-complexity amplification system. Mutual interference is becoming more and more serious, and the dynamics of the reaction system is becoming more and more complex. Therefore, exploring more complex STR loci without reducing specificity and sensitivity is crucial for forensic individual identification

On the other hand, due to the unique characteristics of Y chromosome STR genetic markers, it can only be used in the family screening of male individuals in practical applications, but it cannot distinguish male individuals who are related by blood in the same family

Images