Breeding method for prolongation of rice fertility stage

A technology of rice growth period and growth period, applied in the field of rice biotechnology breeding, can solve the problems of restricting the mechanization process of hybrid rice seed production, inconsistent growth period, short growth period of sterile line, restorer line, etc.

Active Publication Date: 2014-08-27
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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Problems solved by technology

However, at present, the growth period of parents of most strong and superior hybrid rice combinations is inconsistent, mainly because the growth period of the sterile line is shorter than that of the restorer line, which seriously restricts the mechanization process of hybrid rice seed production.

Method used

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  • Breeding method for prolongation of rice fertility stage

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Embodiment Construction

[0031] The test methods used in the following examples are conventional methods unless otherwise specified. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0032] The breeding method adopted in one embodiment of the present invention is introduced below.

[0033] 1. Preparation of a recombinant vector for rice Ehd3 gene targeting.

[0034] 1.1, select the nucleotide sequence GCCCCCCACCACCGCGCAAG at positions 17-39 after the translation initiation codon ATG on the first exon of the rice Ehd3 gene (LOC_Os08g01420) AGG , (the underlined part is the 5'-(N) X -NGG part in the NGG-3' structure), as the targeting site.

[0035] 1.2. Synthesize (BGI) forward oligonucleotide chain (Ehd3KO1P1) and complementary reverse oligonucleotide chain (Ehd3KO1P2) according to the selected target site,

[0036] The specific sequence is:

[0037] Ehd3KO1P1: TGTG GCCCCCCACCACCGCGCAAG;

[0038] Ehd3KO1P2: AAAC C...

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Abstract

The invention relates to a breeding method for prolongation of rice fertility stage, and the breeding method comprises the following steps: selecting a target fragment in a rice fertility stage determining gene Ehd3 exon region and constructing a plant CRISPR / Cas9 targeting recombinant vector, introducing into rice cells for regeneration to sprout; using an expression frame in the vector to realize shearing of Ehd3 exon region DNA in the rice cells so as to cause self healing of the rice cells; sequencing regeneration strain genome target fragments to obtain an afunction-mutational strain carrying two equipotential Ehd3 genes, and confirming the prolongation of the regeneration plant fertility stage by phenotypic identification. Experiments show that the method can quickly obtain fertility stage prolonged rice materials.

Description

technical field [0001] The invention belongs to the field of rice biotechnology breeding, in particular to a breeding method for extending the growth period of rice. Background technique [0002] Rice is the largest food crop in my country, and the current rice planting area is 30 million hectares 2 The planting area of ​​hybrid rice accounts for 57% of the total rice area, and the planting area of ​​hybrid rice is 180,000 hm 2 about. The quality of hybrid rice seeds is the basis for realizing high-quality, high-yield and high-efficiency rice production, and the production level of hybrid rice seeds is directly related to my country's food security. [0003] The production of hybrid rice seeds is the outcrossing cultivation of rice, that is, the male sterile line of hybrid rice is used as the female parent, and the male sterile restorer line is used as the male parent, and they are planted alternately according to a certain row ratio, so that the parents can meet at the fl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00
Inventor 秦瑞英杨剑波魏鹏程李莉马卉李浩杨亚春倪金龙倪大虎宋丰顺陆徐忠
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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