Applications of odorrana grahami polypeptide AH90 and CW49 for promoting skin regeneration
A technology of skin repair and stinky frog, which is applied in the direction of skin diseases, medical preparations containing active ingredients, peptide/protein components, etc., to achieve the effect of promoting repair and reducing the thickness of hyperplasia
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Embodiment 1
[0078] The repairing effect of the skin-repairing peptide AH90 of Frog sativa on the mouse model of skin injury.
[0079] Take 0.05mg / ml stinkfrog to promote skin repair polypeptide AH90 for test, the result is: it can quickly promote the repair function of the skin ( Figure 1-A , Figure 1-B , Figure 1-C , Figure 1-D , Figure 1-E ), the repair effect was more obvious on the second day, scab formed on the fourth day, and basically completely recovered on the sixth day, and there was a significant difference from the skin repair of the control mice.
Embodiment 2
[0081] The repairing effect of the skin-repairing peptide CW49 of Frog sativa on the skin injury model of mice.
[0082] Take 1mg / ml stinkfrog to promote skin repair polypeptide CW49 for test, the result is: it can quickly promote the repair function of the skin ( Figure 1-A , Figure 1-B , Figure 1-C , Figure 1-D , Figure 1-E ), the repair effect was more obvious on the second day, scab formed on the fourth day, and basically completely recovered on the eighth day, and there was a significant difference from the skin repair of the control mice.
Embodiment 3
[0084] Slice diagram of the skin repairing effect of the fingerless frog skin repairing peptide AH90 on mice.
[0085] During the recovery process of the mouse skin, the daily recovery effect was sliced to observe the growth of the skin epidermis. Figure 3-A , Figure 3-B , Figure 3-C , Figure 3-D , Figure 3-E , Figure 3-F It was shown that granulation tissue was formed on the third day after adding AH90 treatment, the epidermal tissue was basically completely recovered on the fifth day, and the thickness of the epidermal tissue in the treatment group was significantly reduced compared with the control group on the ninth day.
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