Method for information storage with DNA (Deoxyribonucleic Acid)

Abstract

The invention relates to a method for information storage with DNA (Deoxyribonucleic Acid). The method comprises the following steps: (1) converting binary information of an original document of a computer into quaternary information, encoding, converting into a DNA complete sequence, wherein binary codes 00, 01, 10 and 11 are respectively and correspondingly converted into four deoxyribonucleotides of A, T, C and G; (2) separating the DNA complete sequence into a plurality of DNA fragments, and organizing and establishing an output DNA sequence which is 90-110nt in length and comprises an interpolation nucleotide encoding sequence consisting of DNA fragments, side primer sequences at two ends, and index encoding sequences on the inner sides of the primer sequences; (3) according to the output DNA sequence, synthesizing an artificial DNA sequence, and storing the artificial DNA sequence. The method provided by the invention has the remarkable advantages of being good in universality, being capable of simplifying calculation, improving continuity, storage efficiency and density of DNA information storage, reducing fault rates, lowering sequence synthesis and detection cost, and the like.

Description

technical field [0001] The invention belongs to the technical field of information storage, and in particular relates to a method for storing information by using artificially synthesized DNA. Background technique [0002] In recent years, global digital information is experiencing explosive growth. It is estimated that by 2017, the demand for global digital preservation data will exceed 16 Zettabytes (ZB, zettabytes). Therefore, it is urgent to develop a reliable, large-scale digital information management information storage media. However, the capacity of existing storage media cannot keep up with the growth rate of digitized information. At present, the main storage media are magnetic and optical media: magnetic media is the most dense storage form currently available on the market, tapes can store up to 185TB of data, and the storage density is about 10GB / mm 3 ;Recently, there are research reports that optical discs store 1PB data, about 100GB / mm 3 Feasibility of sto...

AI Technical Summary

Problems solved by technology

[0007] The above-mentioned method adopts binary and ternary digital information storage to be universal, but the storage methods based on binary and ternary coding methods have low degree of information compression, complicated storage algorithm operation, and poor storage continuity (rotary coding method, information writing method, etc.) After input, the information behind the writing position will change accordingly, resulting in discontinuous storage), and the length of the output DNA is too long, only a single index, the process of DNA synthesis and information recovery is error-prone, and at the same time, the quadruple overlapping walking structure causes data Redundancy, increasing storage costs

Long coding sequences make sequence synthesis, sequencing, and reading difficult, thus hindering their practical application [5]

[0008] In order to overcome the above problems, the applicant proposed a new information storage and reading method for artificially synthesized DNA storage media based on previous research (patent number: ZL201510138778.3). The method of combining some digitized information with base sequence index tables and Unicode realizes the conversion of English, Chinese, numbers, punctuation marks to DNA base sequences. Compared with traditional DNA storage technology, this method has greatly improved, but this method also has Big limitations, this method needs to perform Unicode sequence transcoding when it involves the encoding of multilingual information such as Chinese, the encoding method is complex, and the generated sequences are lengthy, resulting in low storage density and increasing the DNA in the storage process. The cost of sequencing by synthesis

Moreover, this method has poor versatility, is limited to the storage of text, and cannot realize DNA storage and reading of pictures, audio, etc.

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