Simple, convenient, efficient and universal plant chloroplast genome sequencing method

Abstract

The invention discloses a simple, convenient, efficient and universal plant chloroplast genome sequencing method. The method comprises the following steps: directly performing high-flux sequencing by adopting genome DNA (Deoxyribonucleic Acid); capturing chloroplast reads by using a biology bioinformatics method; then, assembling and splicing to obtain a chloroplast genome complete sequence. Compared with the conventional method, the method has the advantages that a high-flux sequencing library is built directly by taking leaf genome DNA as a template, so that the efficiency and universality of the method are improved greatly; complicated PCR (Polymerase Chain Reaction) product sequencing and splicing and assembling of clone segments are not required, and splicing is directly performed from the start by using the reads obtained after high-coverage sequencing, so that test steps are reduced greatly, the test flow is simplified, and the method is simple, convenient, rapid and efficient.

Description

technical field [0001] The invention relates to the field of biological sciences, in particular to a simple, efficient and general method for sequencing plant chloroplast genomes. Background technique [0002] Chloroplast is a unique organelle in green plant cells, the place where cells carry out photosynthesis, and the original source of all energy. Chloroplasts have their own DNA and genetic systems, which are closely related to biological evolution and cell origin. Chloroplast genomes are important research objects in the fields of molecular evolution, phylogeny and molecular markers. Obtaining the complete sequence of chloroplast genomes of target organisms has become a The primary goal of researchers in this field. Traditional plant chloroplast genome sequencing usually uses long-chain PCR with universal primers or the chloroplast genome sequencing strategy of the chloroplast genome library, which generally requires a large amount of fresh materials to extract relative...

AI Technical Summary

Problems solved by technology

Traditional plant chloroplast genome sequencing usually uses long-chain PCR with universal primers or the chloroplast genome sequencing strategy of the chloroplast genome library, which generally requires a large amount of fresh materials to extract relatively high-purity chloroplast DNA. Isolation of high-purity chloroplast DNA needs to optimize the system according to the characteristics of different plant species, which is difficult and has poor versatility, which often becomes the limiting factor for chloroplast genome sequencing

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