Lactobacillus rhamnosus, cultivation of lactobacillus rhamnosus and microcapsule method

A technology of Lactobacillus rhamnosus and microcapsules, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., to achieve the effects of high lactic acid production concentration, improved gastrointestinal tract, and short growth cycle

Inactive Publication Date: 2013-04-10
哈尔滨美华生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Jeremy et al. reported that LGG can regulate the secretion of cytokines such as tumor necrosis factor. The results of the external experiment on the intestinal microbiota of mice showed that LGG can prevent macrophages from secreting excessive tumor ne

Method used

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  • Lactobacillus rhamnosus, cultivation of lactobacillus rhamnosus and microcapsule method
  • Lactobacillus rhamnosus, cultivation of lactobacillus rhamnosus and microcapsule method
  • Lactobacillus rhamnosus, cultivation of lactobacillus rhamnosus and microcapsule method

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Experimental program
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Embodiment 1

[0036] The percentages involved in this embodiment are all percentages by weight.

[0037] 1. First collect samples from the feces of normal adults, sterilize the sampling tools and various sampling containers, and perform multi-point sampling.

[0038] 2. Dilute the collected samples: mix the collected samples evenly under sterile conditions, weigh 20 grams of samples, dissolve them in 100 mL of sterile water, which contains 80-100 glass beads, and shake vigorously for 10 minutes , stand still for one minute, draw 1mL supernatant with a 1mL sterile pipette, transfer to a 9mL sterile water test tube, shake thoroughly, then take 1mL sample from this diluted sample and transfer it to a 9mL sterile test tube, shake thoroughly Evenly, dilute in turn until it reaches 10 -5 .

[0039] 3. Coating and separation: Take out 0.1mL samples from the above-mentioned diluted samples with 0.1mL sterile pipettes, transfer them into the BCP agar plate medium, use a sterile triangular scraper ...

example 1

[0055] The culture method of Lactobacillus rhamnosus LC-STH-13, medium formula: solution is water, glucose 65.0g / L, yeast powder 15.0g / L, beef powder 28.0g / L, soybean peptone 30.0g / L, KH 2 PO 4 3.0g / L, anhydrous sodium acetate 3.0g / L, ammonium citrate 2.0g / L, Tween 801.5mL / L, MgSO 4 ·7H 2 O 1.0g / L, MnSO 4 4H 2 O 0.5g / L, whey powder 19.5g / L, fructooligosaccharides 18.5g / L, casein hydrolyzate 9.5g / L, pH6.5, culture temperature 37℃, culture time 16h.

[0056]After the cultivation, centrifuge at 5000rpm, and then add lyoprotectant for microcapsule embedding. Glutamic acid 50.0g / L. The mass ratio of the embedding agent to the wet bacteria slime is 1:1. After mixing evenly, freeze-dry to obtain the freeze-dried bacteria powder. The number of viable bacteria in the freeze-dried bacteria powder is ≥1.0×10 10 cfu / g.

example 2

[0058] Lactobacillus rhamnosus LC-STH-13, the culture method is as follows: the solution is water, the medium formula glucose 10.0g / L, yeast powder 5.0g / L, beef powder 5.0g / L, soybean peptone 2.0g / L, KH 2 PO 4 0.5g / L, anhydrous sodium acetate 0.1g / L, ammonium citrate 0.1g / L, Tween 800.5mL / L, MgSO 4 ·7H 2 O0.1g / L, MnSO 4 4H 2 O 0.2g / L, whey powder 10.0g / L, fructooligosaccharides 10.0g / L, casein hydrolyzate 5.0g / L, pH4.5, culture temperature 32℃, fermentation time 36h; after the culture, 5000rpm Centrifuge, then add lyoprotectant for microcapsule embedding, microcapsule embedding agent formula: skimmed milk powder 50.0g / L, glycerin 10.0g / L, trehalose 50.0g / L, γ-polyglutamic acid 10.0g / L; the mass ratio of embedding agent and wet bacteria slime is 1:1, after mixing evenly, freeze-dry to obtain freeze-dried bacteria powder, the number of live bacteria of freeze-dried bacteria powder is ≥1.0×10 10 cfu / g.

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Abstract

The invention discloses lactobacillus rhamnosus, cultivation of the lactobacillus rhamnosus and a microcapsule method. The bacterial strain is preserved in China General Microbiological Culture Collection Center (CGMCC); a registering number is CGMCC NO.3994; a Latin name of the bacterial strain is Lactobacillus rhamnosus LC-STH-13; morphological characteristics are gram-positive bacteria, short-rod bacterial thallus, no motion, no gemma, circular bacterial colony, milk white, tidy edges, smooth surfaces and protuberant cores. The invention further discloses a 16S ribosome DNA (Deoxyribose Nucleic Acid) (16S rDNA) complete sequence of the lactobacillus rhamnosus. The lactobacillus rhamnosus has the advantages that a culture is short in growth cycle, high in breeding speed and- produced lactic acid concentration. The lactobacillus rhamnosus can -colonize and survive in an intestinal tract, improves a mucosal barrier function of a gastrointestinal tract, has a function of improving the gastrointestinal tract, and restrains the colonization of harmful bacteria; and after being embedded by a microcapsule, the lactobacillus rhamnosus has stronger ability to resist the poor environment.

Description

technical field [0001] The invention provides a Lactobacillus rhamnosus LC-STH-13 and its culture and microencapsulation methods. Background technique [0002] Lactobacillus rhamnosus LC-STH-13 belongs to the genus Lactobacillus, short bacillus; immobile, no spores, no plasmid, Gram stain positive; facultative anaerobic bacteria, growth temperature range 2~53℃ , the optimum temperature is generally 30-40°C; acid-resistant, the optimum pH is usually 5.5-6.2; generally it can grow at a pH of 5 or lower. In the 1980s, it was isolated from the intestines of healthy people by two American scientists, Gorbach and Goldin, and named Lactobacillus rhamnosus GG (Lactobacillus rhamnosus GG) LGG. In recent years, a large number of animal experiments and human clinical experiments have proved that LGG has considerable physiological functions and its own advantages, and is currently one of the most researched probiotics in the world. [0003] Physiological and biochemical characteristic...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/10C12N11/04C12R1/225
Inventor 梁金钟肖文娟
Owner 哈尔滨美华生物技术股份有限公司
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