8results about "Library linkers" patented technology

The present invention provides a method for constructing a cyclic compound library that overcomes the limitations of conventional ring-closing methods for cyclic compound libraries, has the advantages of milder ring-closing reaction conditions and high generality, can be used to construct monocyclic and bicyclic compound libraries, and involves few side reactions. [Solution] A cyclic compound library and its construction method are provided, which uses a solid support, a molecule containing a photocleavable group, a linker, a building block, a ring-closing A-terminal molecule, and a ring-closing B-terminal molecule at both ends of the reaction synthesis, and utilizes the decomposition of the solid support under light irradiation to complete ring closure of the amino acid residue structures of the ring-closing A-terminal molecule A and the ring-closing B-terminal molecule B through the action of cyclohydrolase. This method, which uses mild ring closure conditions, is more universal and expands the types of chemical reactions and the diversity of the encoded compound library.

The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by blocking the 3′ ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing, by exonuclease treatment and optionally blocking the 3′ ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing, by exonuclease treatment after protective adapters are ligated to target polynucleotides to degrade unincorporated adapters prior to amplification and sequencing, and / or by modifying or blocking 5′ and 3′ ends of pooled indexed polynucleotides from multiple samples, with an optional exonuclease treatment, prior to amplification and sequencing.

The present invention provides a method for constructing a cyclic compound library that overcomes the limitations of conventional ring-closing methods for cyclic compound libraries, has the advantages of milder ring-closing reaction conditions and high generality, can be used to construct monocyclic and bicyclic compound libraries, and involves few side reactions. [Solution] A cyclic compound library and its construction method are provided, which uses a solid support, a molecule containing a photocleavable group, a linker, a building block, a ring-closing A-terminal molecule, and a ring-closing B-terminal molecule at both ends of the reaction synthesis, and utilizes the decomposition of the solid support under light irradiation to complete ring closure of the amino acid residue structures of the ring-closing A-terminal molecule A and the ring-closing B-terminal molecule B through the action of cyclohydrolase. This method, which uses mild ring closure conditions, is more universal and expands the types of chemical reactions and the diversity of the encoded compound library.

Provided is a 5'-end ligation-based single-stranded DNA (ssDNA)-specific high-throughput sequencing method, relating to the technical field of biology. The present disclosure specifically comprises a method for preparing a 5'-end ligation-based ssDNA-specific sequencing (Liss-seq) library. The method comprises the following steps of treating a sample to be tested: 1) using a DNA polymerase without exonuclease activity to fill in 5' ends of double-stranded DNA (dsDNA); 2) adding a tail to the 3' end; (3) ligating a renatured 5' hairpin adaptor; a structure of the 5' hairpin adaptor being: overhang-random base region-first stem region-loop region-second stem region, and the first stem region and the second stem region forming a double strand by means of renaturation; and 4) carrying out amplification on a ligated product. The amplified product constitutes a ssDNA sequencing library.

Provided herein are compositions and methods for normalizing sequencing libraries.Further provided herein are adapter conjugates and hybrid circular adapters, compositions comprising them, and methods for producing them.Further provided herein are methods for normalizing genomic DNA libraries for next-generation sequencing using adapter conjugates, hybrid circular adapters, and compositions comprising them.

A method for preparing a 5′-end ligation-based ssDNA-specific sequencing (Liss-seq) library is provided. The method includes: (a) treating a DNA sample to be tested that includes single-stranded DNA and double-stranded DNA with Klenow Fragment (3′→5′exo−) DNA polymerase to fill in 5′ ends of the double-stranded DNA to obtain a first reaction product; (b) conducting a 3′ end tail addition reaction on the first reaction product to obtain a second reaction product; (c) ligating the second reaction product with a renatured 5′ hairpin adaptor to obtain a ligation product; a structure of 5′ hairpin adaptor being: 5′-overhang-random base region-first stem region-loop region-second stem region-3′, the first stem region and the second stem region forming a double-strand through a renaturation treatment; and (d) amplifying the ligation product to obtain the Liss-seq library, a length of the overhang being within a range of 0-20 nt.

Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest.

The present disclosure provides, in part, electrochemical sensors and aptamers for detecting phenol-soluble modulins (PSMs) and methods of preparing and using the same, e.g., for detecting Staphylococcus spp.