Application of two dsRNAs (double-stranded ribonucleic acids) and combination thereof in controlling aphid damage

An aphid and DNA molecule technology, applied in DNA/RNA fragments, applications, recombinant DNA technology, etc., can solve the problems of unclear resistance mechanism, ineffective conventional breeding, and lack of aphid-resistant germplasm resources in wheat
CN103088024AActive Publication Date: 2013-05-08INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Publication Date
2013-05-08

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Abstract

The invention discloses application of two dsRNAs (double-stranded ribonucleic acids) and combination thereof in controlling aphid damage. The dsRNA provided by the invention is disclosed as 1), 2) or 3): 1) dsRNA composed of nucleotide disclosed as Sequence 4 in the sequence table and nucleotide disclosed as reverse complementary sequence thereof; 2) dsRNA composed of nucleotide disclosed as Sequence 5 in the sequence table and nucleotide disclosed as reverse complementary sequence thereof; and 3) composed of the following two dsRNAs: dsRNA disclosed as 1) and dsRNA disclosed as 2). The experiment proves that dsRNAs of grain aphid growth and development related gene 23028 and salivary secretion protein gene coo2 are obtained, and the grain aphid in-vivo growth and development related gene 23028 and salivary secretion protein gene coo2 are silenced by an in-vitro dsRNA feeding method by using an RNAi (ribonucleic acid interference) technique, thereby resulting in hindrance of grain aphid growth and development and generating lethal effect.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to the application of two dsRNAs and their combination in controlling aphid damage. Background technique

[0002] Wheat aphids (especially wheat aphids) are one of the main pests that harm wheat production in China. According to statistics, the area of ​​wheat aphid damage in China can reach as high as 17 million hectares every year, accounting for 62% of the total wheat planting area, resulting in a 15% reduction in production— 30%, up to 50% in severe cases. In recent years, due to factors such as global warming and changes in farming systems, the reproductive ability and adaptability of aphids have been significantly enhanced, and their harm has become increasingly serious. At present, aphid control is mainly based on spraying pesticides, but a large amount of pesticides are not only harmful to humans and animals, but also cause serious environmental pollution. Breeding aphid-resis...

Claims

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