Nuclein acid fixing method and method for producing biosensor by said method

Abstract

A superior adsorption method for immobilizing nucleic acid probe on the surface of a solid phase substrate is provided. A nucleic acid immobilization method for immobilizing nucleic acid on a solid phase substrate comprising: bringing the above-mentioned solid phase substrate into contact with a composition comprising a total concentration of 0.1 to 2 mu M of a nucleic acid as a probe and a compound or a salt thereof, the compound being represented by the following formula: HS-L<1>-L<2>-R wherein L<1> is a single bond or a C1-15 alkylene group; L<2> is a single bond, nucleic acid, a polyethylene glycol group, -CO-NH-, or -NH-CO-; R is a hydroxyl group, an amino group, a ferrocenyl group, or a carboxyl group; provided that neither L<1> nor L<2> is a single bond, and incubating the composition in contact with a surface of the solid phase substrate.

Description

technical field

[0001] The invention relates to a method for immobilizing nucleic acid, a method for manufacturing a biosensor and a method for detecting nucleic acid using the method. Background technique

[0002] As methods for detecting target nucleic acid molecules, analyzing sequences, and gene mapping, biosensors such as microarray technology using nucleic acid probes have been widely used in recent years. A typical hybridization method uses a nucleic acid probe that utilizes the interaction (hybridization) of complementary nucleic acid strands to capture a target nucleic acid molecule, and directly or indirectly (for example: detection of the luminescence intensity of a fluorescent molecule attached to a sample) discriminates the target nucleic acid molecule. All or part of the nucleic acid sequence is analyzed for the presence of the target nucleic acid molecule. Generally, a single-stranded nucleic acid molecule having all or part of the target nucleic acid molecul...

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