Immobilized nucleic acid probe for non-labeling detection

Abstract

The invention discloses a solid-phase nucleic acid probe used to non-marking detection, including solid carrier, on which the scant nucleotide probe, with the fluorescence group, the fluorescence cancellation group and the identification sequence snippet of the peculiar nucleic acid is fixed, and the fluorescence cancellation group can be cancel the fluorescence; after intercrossing the group andthe snippet in the scant nucleotide probe, by the biochemical reaction, separate the two groups in the scant nucleotide probe to make the probe emit the fluorescence.

Description

1. Technical field:

[0001] The invention relates to a solid-phase nucleic acid probe, in particular to a solid-phase nucleic acid probe which can be used for non-label detection. 2. Background technology

[0002] With the deepening of genome research, it will become possible to understand the differences in life, the rules of disease occurrence and development, and the interaction between drugs and living organisms at the genetic level. The high-throughput detection and analysis technology of nucleic acid sequence information will become one of the core technologies in the field of life sciences such as medicine. Recently, gene chip technology has attracted more and more attention. However, there are still some problems in gene chip technology, which affect its application in biology and clinical medicine. 1) The detection process is complicated. The extracted sample DNA needs to be amplified and incorporated with fluorescent markers. In this process, cross-contamination...

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