Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Terahertz flow cytometric sensor and its detection method for label-free detection of single or a small number of living cells

A technology of flow cytometry and detection method, applied in the field of sensors, can solve the problems such as the inability to achieve uniform wrapping of a single or a small number of cells, and the difficulty in ensuring that only one "cell droplet" passes through the detection place, and achieves obvious effects.

Active Publication Date: 2019-03-19
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] For problem 1: Since OCA is mostly an organic solvent, it is difficult for hydrophilic cells to disperse directly in OCA, and it can only be made into an emulsion for detection. However, the traditional method of stirring and shaking cannot achieve a single or uniform wrapping of a small number of cells;
[0012] For problem 2: Microfluidic technology can be used to allow OCA to wrap cells to form "cell droplets" to pass through the THz detection site, but it is difficult to ensure that only one "cell droplet" passes through the detection site for each detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Terahertz flow cytometric sensor and its detection method for label-free detection of single or a small number of living cells
  • Terahertz flow cytometric sensor and its detection method for label-free detection of single or a small number of living cells
  • Terahertz flow cytometric sensor and its detection method for label-free detection of single or a small number of living cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A terahertz flow cytometric sensor for label-free detection of single or small numbers of living cells, such as figure 1 As shown, the sensor includes a cell droplet making module, a microfluidic detection channel, and a THz detection module. The cell droplet making module includes a microfluidic lumen, and the microfluidic lumen includes a microfluidic lumen for delivering a cell suspension. Inlet channel a for transporting OCA and two inlet channels b symmetrically connected with inlet channel a, the intersection of the three inlet channels is connected with outlet channel d for transmitting cell droplets, the THz detection module includes a A detection tank with the same fluid detection channel size, the outlet channel d is connected to the detection tank through a microfluidic detection channel; the two inlet channels b and the inlet channel a form a T-shaped connection structure; the two inlet channels b and the inlet The inner diameter of channel a is 20 microns, ...

Embodiment 2

[0055] Such as Figure 4 As shown, the difference between embodiment 2 and embodiment 1 is that the two inlet channels b and the inlet channel a of the cell droplet production module are connected in a Y-shaped structure. The flow path of OCA in the lumen is reduced, the cell suspension is easier to form cell droplets, and the effect is better.

Embodiment 3

[0057] Such as Figure 5 As shown, the difference between Example 3 and Example 1 is that the microfluidic detection channel is designed as a U-shaped structure. This structure increases the length of the microfluidic, and it is easier to disperse the cell droplets, making it easier for the cell droplets to sequentially With THz detection area, the effect is even better.

[0058] Screening of OCA with good biocompatibility and small absorption in the THz frequency band

[0059] 1) Biocompatibility experiment of 8 common OCAs: CCK8 cell proliferation-toxicity detection test was used to evaluate 8 common OCAs (azone, glycerol, oleic acid, 1 , 2-propanediol (C 3 h 8 o 2 ), dipropylene glycol (C 6 h 14 o 3 ), isopropyl myristate, polyethylene glycol and simethicone) for biocompatibility. Because the THz spectrum detection process takes a short time, the sample detection process can be completed within 2 minutes, so the above 8 substances were respectively applied to the ad...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a terahertz flow cytometry sensor for label-free detection of single or small quantity of living cells and a detection method of the terahertz flow cytometry sensor. The sensor comprises a cell microdroplet preparation module, a microfluid detection passage and a THz detection module. The cell microdroplet preparation module comprises at least one microfluid tube cavity which comprises an inlet passage a and two inlet passages b, the inlet passage a is used for transferring cell suspension, the two inlet passages b are used for transferring an OCA (optical clearing agent) and in symmetrical linkage with the inlet passage a, and an outlet passage d used for transferring cell microdroplets is connected at the joint of the three inlet passages. The THz detection module comprises at least one detection groove identical to the microfluid detection passage in size, and the outlet passage d is connected with the detection groove through the microfluid detection passage. By the terahertz flow cytometry sensor, the single or small quantity of living cells can be uniformly wrapped in the OCA to form independent 'cell microdroplets' sequentially passing a THz detection position, so that label-free detection of the single or small quantity of living cells is realized.

Description

technical field [0001] The invention belongs to the technical field of sensors, and in particular relates to a terahertz flow cytometry sensor for label-free detection of a single or a small number of living cells and a detection method thereof. Background technique [0002] The analysis of the structure and function of living cells is one of the core issues in the field of biomedicine today. From the morphological structure of cells to the quality and quantity of intracellular biological macromolecules, the detection of relevant information of living cells is the basis for revealing the pathophysiological processes of higher life levels such as tissues and organs. It has been widely used in early diagnosis of tumors, Various research fields such as classification of blood system diseases. According to whether markers are used in the detection process, live cell detection can be divided into labeled detection represented by traditional cytochemical staining, molecular tomog...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/14
CPCG01N15/1434
Inventor 杨柯府伟灵罗阳杨翔张阳林钟劝赵祥刘羽余闻静黄娇祺刘伟
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com