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Device and method for label-free detection of Escherichia coli O157:H7 DNA based on LAPs and ZNO nanorod arrays

A nanorod array, Escherichia coli technology, applied in microorganism-based methods, biochemical equipment and methods, microbial assay/inspection, etc., can solve problems such as DNA hybridization effects, achieve highly sensitive label-free DNA detection, and easy to operate , the effect of low detection limit

Active Publication Date: 2020-11-10
XI AN JIAOTONG UNIV
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Problems solved by technology

At present, the non-labeled method of detecting DNA generally uses the chip to modify the complementary strand to directly capture the target DNA molecule, which is simple and fast, but the flat structure may have a certain impact on DNA hybridization

Method used

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  • Device and method for label-free detection of Escherichia coli O157:H7 DNA based on LAPs and ZNO nanorod arrays
  • Device and method for label-free detection of Escherichia coli O157:H7 DNA based on LAPs and ZNO nanorod arrays
  • Device and method for label-free detection of Escherichia coli O157:H7 DNA based on LAPs and ZNO nanorod arrays

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Embodiment Construction

[0048] Below in conjunction with accompanying drawing, the present invention will be further described:

[0049] figure 1 is the measuring device used in the present invention. Including Faraday shielding box 1, loading platform 2, semiconductor laser 3, LAPS sensor 4, constant voltage device 5, lock-in amplifier 6, acquisition card 7 and computer; wherein, loading platform 2 is placed in Faraday shielding box 1, semiconductor laser 3 Installed below the loading platform 2, the LAPS sensor 4 is installed on the loading platform 2, the electrode connection on the potentiostat 5 and the lock-in amplifier 6 and the LAPS sensor 4, the lock-in amplifier 6 and the acquisition card 7 are connected, and the acquisition card 7 is installed on the The computer 8 is used to collect the photocurrent of the LAPS sensor 4 .

[0050] see figure 2 , LAPS sensor 4 comprises alloy plate 10, and the middle position of alloy plate 10 is successively provided with Si layer 14, SiO 2 layer 15 ...

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Abstract

The invention discloses a colibacillus O157:H7DNA label-free detection device and method based on LAPS and ZnO nanorod array. The device comprises a Faraday shield box, a loading table, a semiconductor laser, an LAPS sensor, a manostat, a lock-in amplifier, an acquisition card, and a computer. The method comprises of performing bacterial culture, extracting colibacillus O157:H7DNA, manufacturing aLAPS sensor and modifying a ZnO nanorod; modifying probe chain DNA on a surface of the LAPS sensor, and scanning an I-V curve; fitting an optimal calibration curve detecting concentration of O157:H7DNA through traversing and a least square method; and analyzing an I-V curve of a to-be-tested sample solution and voltage offset V and substituting into the calibration curve, and calculating concentration of the to-be-tested sample. The method realizes label-free detection of O157:H7 DNA, and has advantages of short time, low cost, convenience in operation, 3D site for O157:H7 DNA hybrid, and high sensitivity.

Description

technical field [0001] The invention relates to the field of detection of Escherichia coli O157:H7 DNA, in particular to a device and method for label-free detection of Escherichia coli O157:H7 DNA based on LAPS and ZnO nanorod arrays. Background technique [0002] The detection of bacterial deoxyribonucleic acid (DNA) has extensive technical significance in the fields of biomedicine, food industry, and environmental control. Water, can cause hemorrhagic colitis, and lead to bloody diarrhea and other symptoms. The frontier of DNA biosensor development is currently based on PCR amplification technology and labeling method; the PCR method is to amplify DNA fragments or sequences through enzymatic synthesis reactions, and it takes a long time to amplify trace amounts of DNA. The labeling method usually uses fluorescence to label probes or target DNA molecules. DNA production costs are high and the detection process is complicated. Therefore, it is highly necessary to develop ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48C12Q1/689C12Q1/6806C12Q1/10C12R1/19
CPCC12Q1/6806C12Q1/689G01N27/48C12Q2531/107
Inventor 吴春生田玉兰朱平陈雅婷杜立萍
Owner XI AN JIAOTONG UNIV
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