Preparation and application of unmarked electrochemiluminescent tumor marker immunosensor
A tumor marker, immunosensor technology, applied in the field of new nano functional materials and biosensors, can solve the problems of unfavorable luminescence system popularization and harsh reaction conditions, achieve important scientific significance and application value, improve sensitivity and accuracy , the effect of convenient operation
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Embodiment 1
[0035] Example 1 Preparation of AuAgNRs-Lum-Chi.
[0036] Mix 20 mL of AuNRs and 20 mL of 0.05 mol / L CTAB solution, stir at 30 °C, and sequentially add 3 mL of 0.1 mol / L AA solution, 0.5 mL of 0.05 mol / L AgNO 3 solution and 3mL 0.1 mol / L NaOH solution, stop stirring, let it stand for 1h, centrifuge, and redisperse the product in 20mL water to obtain AuAgNRs.
[0037] Mix 3 mL of AuAgNRs and 3 mL of 1×10 -3 mol / L Lum solution was mixed, stirred for 1 h, centrifuged, and the product was redispersed into 5 mL chitosan solution with a mass fraction of 0.3 % to obtain AuAgNRs-Lum-Chi solution.
[0038] The AuNRs described are 20 nm long rod-shaped AuNRs in aqueous solution.
[0039] The Chi solution is prepared by adding pure Chi to 1% acetic acid by volume.
Embodiment 2
[0040] Example 2 Preparation of AuAgNRs-Lum-Chi.
[0041] Mix 20 mL of AuNRs and 20 mL of 0.1 mol / L CTAB solution, stir at 38 °C, and sequentially add 5 mL of 0.1 mol / L AA solution, 0.8 mL of 0.05 mol / L AgNO 3 solution and 5mL 0.1 mol / L NaOH solution, stop stirring, let stand for 2h, centrifuge, and redisperse the product in 20mL water to obtain AuAgNRs aqueous solution.
[0042] Mix 4 mL of AuAgNRs and 4 mL of 1×10 -3 mol / L Lum solution was mixed, stirred for 2 h, centrifuged, and the product was redispersed into 5 mL chitosan solution with a mass fraction of 0.5 % to obtain AuAgNRs-Lum-Chi solution.
[0043] The AuNRs described are 30 nm long rod-shaped gold nanoparticles in aqueous solution.
[0044] The Chi solution is prepared by adding pure Chi to 1% acetic acid by volume.
Embodiment 3
[0045] Example 3 Preparation of AuAgNRs-Lum-Chi.
[0046] Mix 20 mL of AuNRs and 20 mL of 0.2 mol / L CTAB solution, stir at 45 °C, and sequentially add 6 mL of 0.1 mol / L AA solution, 1 mL of 0.05 mol / L AgNO 3 solution and 6mL 0.1 mol / L NaOH solution, stop stirring, let stand for 3h, centrifuge, and redisperse the product in 20mL water to obtain AuAgNRs.
[0047] Mix 5 mL of AuAgNRs and 5 mL of 1×10 -3 mol / L Lum solution was mixed, stirred for 3 h, centrifuged, and the product was redispersed into 5 mL chitosan solution with a mass fraction of 0.7 % to obtain AuAgNRs-Lum-Chi solution.
[0048] The AuNRs described are 40 nm long rod-shaped gold nanoparticles in aqueous solution.
[0049] The Chi solution is prepared by adding pure Chi to 1% acetic acid by volume.
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