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A label-free electrochemiluminescent aptasensor for detecting carcinoembryonic antigen and its preparation method and use method

An aptamer sensor and carcinoembryonic antigen technology, applied in scientific instruments, instruments, measuring devices, etc., can solve the problems of easy inactivation of biological enzymes, narrow linear range, etc., and achieve good specificity, wide detection range, and high sensitivity Effect

Active Publication Date: 2021-01-26
SHANGQIU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common methods for detecting carcinoembryonic antigen include fluorescence immunoassay (FIA), enzyme-linked immunoassay (ELISA), etc., but these methods have some shortcomings, such as narrow linear range, easy inactivation of biological enzymes, etc.

Method used

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  • A label-free electrochemiluminescent aptasensor for detecting carcinoembryonic antigen and its preparation method and use method
  • A label-free electrochemiluminescent aptasensor for detecting carcinoembryonic antigen and its preparation method and use method
  • A label-free electrochemiluminescent aptasensor for detecting carcinoembryonic antigen and its preparation method and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A method for preparing a label-free electrochemiluminescence aptasensor for detecting carcinoembryonic antigen, the steps are as follows:

[0045] (1) Au-Ag / g-C 3 N 4 Preparation of nanocomposites

[0046] Put 20.0 g of urea into an alumina crucible, place it in a muffle furnace, raise the temperature to 550 °C at a rate of 15 °C / min, and maintain this temperature for 2 h. Naturally cooled to room temperature to obtain a yellow solid, after grinding to obtain g-C 3 N 4 Nanosheets.

[0047] Add 5 mg of g-C to a 150 mL three-necked flask equipped with a reflux condenser 3 N 4 Nanosheets, homogeneous g-C was obtained under magnetic stirring 3 N 4 Add 50 μL of 0.1 M chloroauric acid solution and 40 μL of 0.1 M silver nitrate solution to the suspension, heat to boiling and quickly add a mixed solution of 20 μL of 0.1 mM sodium borohydride and 250 μL of 0.5 mM sodium citrate, and continue heating After reflux for 15 min, naturally cool to room temperature under stirr...

Embodiment 2

[0054] A method for preparing a label-free electrochemiluminescence aptasensor for detecting carcinoembryonic antigen, the steps are as follows:

[0055] (1) Au-Ag / g-C 3 N 4 Preparation of nanocomposites

[0056] Put 20.0 g of urea into an alumina crucible, place it in a muffle furnace, raise the temperature to 550 °C at a rate of 15 °C / min, and maintain this temperature for 2 h. Naturally cooled to room temperature to obtain a yellow solid, after grinding to obtain g-C 3 N 4 Nanosheets.

[0057] Add 5 mg of g-C to a 150 mL three-necked flask equipped with a reflux condenser 3 N 4 Nanosheets, homogeneous g-C was obtained under magnetic stirring 3 N 4 After the suspension, add 75 μL of 0.1 M chloroauric acid solution and 50 μL of 0.1 M silver nitrate solution, heat to boiling and quickly add 50 μL of 0.1 mM sodium borohydride and 225 μL of 0.5 mM sodium citrate mixed solution, continue heating After reflux for 15 min, naturally cool to room temperature under stirring, ...

Embodiment 3

[0064] A method for preparing a label-free electrochemiluminescence aptasensor for detecting carcinoembryonic antigen, the steps are as follows:

[0065] (1) Au-Ag / g-C 3 N 4 Preparation of nanocomposites

[0066] Put 20.0 g of urea into an alumina crucible, place it in a muffle furnace, raise the temperature to 550 °C at a rate of 15 °C / min, and maintain this temperature for 2 h. Naturally cooled to room temperature to obtain a yellow solid, after grinding to obtain g-C 3 N 4 Nanosheets.

[0067] Add 5 mg of g-C to a 150 mL three-necked flask equipped with a reflux condenser 3 N 4 Nanosheets, homogeneous g-C was obtained under magnetic stirring 3 N 4 After the suspension, add 60 μL of 0.1 M chloroauric acid solution and 30 μL of 0.1 M silver nitrate solution, after heating to boiling, quickly add a mixed solution of 32 μL of 0.1 mM sodium borohydride and 275 μL of 0.5 mM sodium citrate, and continue heating After reflux for 15 min, naturally cool to room temperature u...

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Abstract

The invention belongs to the technical field of functional nano-materials and aptamer sensors, and in particular relates to a label-free electrochemiluminescence aptamer sensor for detecting carcinoembryonic antigen as well as preparation method and usage method thereof. The label-free electrochemiluminescence aptamer sensor for detecting the carcinoembryonic antigen is obtained by attaching Au / Agalloy to g-C3N4 two-dimensional nano-sheets to form an Au / Ag / g-C3N4 nano-composite, using the Au / Ag / g-C3N4 nano-composite as a substrate to modify a glassy carbon electrode, then combining a carcinoembryonic antige aptamer with the Au / Ag / g-C3N4 nano-composite by means of Au-S and Ag-S covalent bonds. The aptamer sensor for the carcinoembryonic antigen, disclosed by the invention, has the advantages of good specificity, wide detection range, high sensitivity, fast detection speed, and capability of detecting practical serum samples with a minimum detection limit of 0.32fg / mL.

Description

technical field [0001] The invention belongs to the technical field of functional nanomaterials and aptamer sensors, in particular to a label-free electrochemiluminescence aptamer sensor for detecting carcinoembryonic antigens and its preparation method and use method. Background technique [0002] Carcinoembryonic antigen (CEA) is an acidic glycoprotein with the characteristics of human embryonic antigen. It is a broad-spectrum tumor marker and has important clinical value for the differential diagnosis of malignant tumors, disease monitoring, and efficacy evaluation. Common methods for detecting carcinoembryonic antigen include fluorescence immunoassay (FIA), enzyme-linked immunoassay (ELISA), etc., but these methods have some shortcomings, such as narrow linear range and easy inactivation of biological enzymes. Therefore, it is necessary to invent a method for detecting carcinoembryonic antigen with good specificity, wide detection range, good stability and low cost. [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3275
Inventor 韦秀华王军梅郝远强张银堂瞿鹏徐茂田
Owner SHANGQIU NORMAL UNIVERSITY
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