Method for synthesizing fluorescent silver nano clusters by taking general DNA (Deoxyribose Nucleic Acid) as stabilizer

A silver nanocluster and stabilizer technology, which is applied in the field of large-scale synthesis of fluorescent silver nanoclusters, can solve the problems of inconvenient large-scale synthesis and high price, and achieve the effects of convenient large-scale synthesis, low cost, and small particle size

Inactive Publication Date: 2013-04-03
HUNAN UNIV OF SCI & TECH
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Problems solved by technology

This method needs to use synthetic DNA as a stabilizer, the price is relatively high, and it is not convenient for large-scale synthesis

Method used

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  • Method for synthesizing fluorescent silver nano clusters by taking general DNA (Deoxyribose Nucleic Acid) as stabilizer
  • Method for synthesizing fluorescent silver nano clusters by taking general DNA (Deoxyribose Nucleic Acid) as stabilizer
  • Method for synthesizing fluorescent silver nano clusters by taking general DNA (Deoxyribose Nucleic Acid) as stabilizer

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Embodiment Construction

[0018] The present invention will be further described in detail below in conjunction with specific experimental examples.

[0019] (1) Preparation of the solution used:

[0020] Dissolve 50mg of protidine DNA in 50mL of water to make a DNA stock solution (store in a refrigerator at 4°C); weigh 0.1690g of AgNO 3 Dissolved in 50mL water to prepare AgNO with a concentration of 20mM 3 stock solution; take 0.0147g trisodium citrate and dissolve it in 50mL water to prepare a concentration of 1.0mM trisodium citrate stock solution; newly prepared NaBH 4 The solution concentration was 2.5 mM.

[0021] (2) Preparation process of silver nanoclusters:

[0022] Take 7.2mL protist DNA stock solution, heat it in a 95°C water bath for 15min, then cool it down to room temperature quickly, add 150μL AgNO 3 stock solution and 38 μL of 1.0 mM trisodium citrate stock solution, and add water to 7.5 mL. The solution was mixed and reacted for 24h (in a refrigerator at 4°C), so that the Ag + F...

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Abstract

The invention belongs to the field of chemical engineering, and particularly relates to a method for large-scale synthesization of fluorescent silver nano clusters by taking general modified DNA (Deoxyribose Nucleic Acid) as a stabilizer. The method mainly comprises the following steps: (1) mixing the general modified DNA with a silver ion solution and reacting in a refrigerator of 4 DEG C for 24 h; and (2) adding a NaBH4 solution into a mixed solution in the step (1) and reacting for 3 h at the room temperature of 10-25 DEG C to obtain a silver nano cluster solution. According to the method, the general modified DNA is taken as the stabilizer for the convenience of large-scale synthesization of the fluorescent silver nano clusters; and the synthetic method is economic, the particle size is smaller, the particle distribution is uniform, and strong fluorescent light can be generated.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a method for large-scale synthesis of fluorescent silver nanoclusters using ordinary denatured DNA as a stabilizer. Background technique [0002] Silver nanoclusters have attracted great attention in the fields of chemistry, biology and material science due to their fluorescent properties. Because of its superior fluorescence properties, this kind of fluorescent silver nanoparticles (F-AgNPs) is also called silver nano quantum dots or nano clusters. F-AgNPs have the characteristics of biocompatibility, large specific surface area, low toxicity, etc., and can emit strong fluorescent signals; they have a wide range of applications in the field of analytical chemistry. [0003] At present, people often use single-stranded DNA polymerized with cytosine to prepare F-AgNPs with strong fluorescence emission. This method needs to use synthetic DNA as a stabilizer, the price...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24
Inventor 龙云飞邝阳芳陈述
Owner HUNAN UNIV OF SCI & TECH
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