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Nosema bombycis for controlling Athetis lepigone and application thereof

A two-point technology for Spodoptera frugiperda and microsporidia, applied in application, microorganism-based methods, microorganisms, etc., can solve problems such as serious damage, high insect population density, pollution, etc., to reduce the use of pesticides, and reduce the number of populations. , the effect of high insecticidal activity

Inactive Publication Date: 2014-12-24
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, from 2011 to 2013, the Noctuid moth broke out in the Huanghuaihai summer corn region, with a large area of ​​occurrence, high population density, and serious damage (Jiang Yuying, China Plant Protection Guide, 2012 02; Liu Jie et al., China Plant Protection Guide, Issue 09, 2013; Li Zhizhi et al., Plant Protection, Issue 01, 2013], has become an important pest affecting summer maize production
[0003] The prevention and control methods of the second-spot moth are mainly chemical control, such as spraying with organophosphorus chemicals, trapping and killing with poisonous soil and poisonous bait, and root irrigation, etc., because the insect has the habit of latent damage, therefore, there are difficulties in chemical control. It is easy to cause phytotoxicity and cause environmental pollution and other problems
[0006] After searching, no report was found on the use of microsporidia to prevent and control the spodopterygii

Method used

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  • Nosema bombycis for controlling Athetis lepigone and application thereof
  • Nosema bombycis for controlling Athetis lepigone and application thereof
  • Nosema bombycis for controlling Athetis lepigone and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, the preparation of silkworm Microsporidia ED2013 spore suspension of the present invention

[0046] Proceed as follows:

[0047] (1), under indoor temperature at 25~27 ℃, light time 14 hours / day, relative humidity 60%~80% condition, use artificial diet to collect from the field Proxenus lepigone (Moschler) adult Carry out subculture and raise 5 generations, form stable indoor breeding colony of Spodoptera spp. wherein said artificial feed and preparation method thereof see patent application " a kind of artificial feed of Spodoptera and its preparation method and application " ( The application number is: 201110404232.x).

[0048] (2), the inoculation propagation propagation of ED2013: be 2 * 10 with concentration 6 Nosema bombycis ED2013 (Nosema bombycis) ED2013 (spore / ml) will be preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms on August 7, 2014, and the preservation address is: Chinese Academy of Sci...

Embodiment 2

[0051] Embodiment 2; Different concentrations of ED2013 spore suspensions are used to prevent and control the two-spot moth contrast test

[0052] Proceed as follows:

[0053] Utilize hemocytometer counting, the ED2013 spore suspension prepared in embodiment 1 is prepared into 1×10 respectively with sterile water 5 , 1×10 6 , 1×10 7 Spores / ml of spore suspension, soak Chinese cabbage leaves, dry the surface water of Chinese cabbage leaves, feed the newly hatched, 2nd, 3rd, and 4th instar larvae of the second-pointed moth, and feed them for 24 hours. A single tube was raised normally, and 30 larvae were treated for each treatment, repeated three times, and treated with sterile distilled water as a control, and the natural mortality rate of the control was within 5% as an effective experiment. Investigate the number of dead insects in each treatment every day, and calculate the corrected mortality rate, lethal medium concentration LC50 and lethal medium time LT50.

[0054] B...

Embodiment 3

[0062] Embodiment 3 Field drug efficacy comparative test of different preparations of No. silkworm ED2013 of the present invention

[0063] (1) Test treatment: (1), Erdianwei Spodoptera control agent: the ED2013 spore suspension prepared in Example 1 is diluted to 1×10 with sterile water 7 Spores / ml, add 0.01% Tween 20 (v / v) and 1% sucrose, be mixed with the two spot moth control agent, carry out spray control, spray 2 times continuously at intervals of 2-3 days.

[0064] (2), poisonous bait: mix wheat bran with a diameter of 2mm and the ED2013 spore suspension prepared in Example 1, and prepare 0.5~1×10 9 Spores / 100g of poisonous bait, so that the water content reaches 10-12%, and the poisonous bait is sprinkled on the roots of corn seedlings to trap and kill them.

[0065] (3), chemical pesticides: chlorpyrifos, spray treatment.

[0066] (4) Blank control: In addition to normal agricultural operations, no insecticidal treatment was performed.

[0067] A field experiment w...

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Abstract

The invention discloses a Nosema bombycis ED2013 for controlling Athetis lepigone, belonging to the field of biological control on crop pests. The Nosema bombycis is collected by China General Microbiological Culture Collection Center on August 7th, 2014, and the collection number is CGMCC No.9557. The invention also discloses a pesticide containing the ED2013 Athetis lepigone and a preparation method thereof. The Nosema bombycis ED2013 has higher pesticidal activity and pesticidal effect for Athetis lepigone larvae, can effectively lower the population quantity of Athetis lepigone, and provides a high-efficiency way for controlling Athetis lepigone. The invention belongs to a biological control method which has no environmental pollution and is harmless to other natural enemies; and the Nosema bombycis ED2013 can substitute the pesticide or lower the pesticide consumption, and is environment-friendly.

Description

technical field [0001] The invention belongs to the field of biological control of crop pests, and in particular relates to a microsporidia silkworm used for preventing and controlling the spodoptera moth; Background technique [0002] Athetis lepigone (scientific name: Proxenus lepigone (Moschler), synonym: Athetis lepigone) is a pest of the genus Spodoptera of Lepidoptera. It mainly harms corn seedlings and is distributed in Japan, North Korea, Russia, Europe and other places. In 2005, the insect was found to harm corn seedlings in Hebei Province (Jiang Jingyu et al., China Plant Protection Guide, No. 7, 2006); subsequently, it was found in Shijiazhuang, Xingtai, Baoding, Hengshui, Handan, Ningjin, Shandong, Xinxiang, Henan and other areas in Hebei Province. The reports of the occurrence of the pest's damage are all in the form of dots and flakes, and the impact is not large. However, from 2011 to 2013, the Noctuid moth broke out in the Huanghuaihai summer corn region, wi...

Claims

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Application Information

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IPC IPC(8): C12N1/10A01N63/00A01P7/04C12R1/90
Inventor 张海剑杜立新石洁曹伟平宋健郭宁
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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