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Method for artificially rearing athetis lepigone for test

A two-point spodoptera, artificial technology, applied in animal feed, animal feed, application and other directions, can solve the problems of reducing the survival rate of larvae, increasing the workload of the staff, increasing the phenomenon of self-mutilation, and improving the survival rate and reproduction rate. , reduce reproductive costs and risks, and improve the effect of feed utilization

Inactive Publication Date: 2013-10-09
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

In the artificial breeding method disclosed in Chinese Patent Publication No. CN102578432B, the larvae of Spodoptera moth are raised in groups, which increases the phenomenon of self-mutilation, greatly reduces the survival rate of larvae, and the moldy feed needs to be removed in a whole box during feeding, which is time-consuming and laborious; The mature larvae need to be transferred out separately for pupation, which increases the workload of the staff; the adult larvae of the two-spot moth have phototaxis, and they are reared in the dark during the entire rearing process, which does not conform to the growth law of insects

Method used

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  • Method for artificially rearing athetis lepigone for test

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Experimental program
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Effect test

Embodiment 1

[0040] A kind of artificial rearing method for testing two o'clock moth, mainly comprises the following steps:

[0041] (1) Choose a fresh-keeping box with a diameter of 14cm and a height of 8cm, put a filter paper with a slightly smaller diameter in it, and drip water on the filter paper to make the filter paper moist;

[0042] (2) Transfer the eggs laid by adults of Spodoptera spp. in the same period of time to the fresh-keeping box described in step (1). After hatching the larvae, transfer each larvae to a container with a diameter of 2 cm and a height of 6 cm. Single-head feeding with synthetic feed blocks in glass tubes;

[0043] (3) After feeding for 20-24 days, the larvae are nearly mature and pupate, and no feed is added; the pupae are transferred to a sterilized petri dish with a diameter of 5 cm and a height of 0.5 cm, with 10 male and 10 larvae in each dish;

[0044] (4) Put the petri dish with pupae in step (3) into a glass bottle with a diameter of 9 cm and a hei...

Embodiment 2

[0053] A kind of artificial breeding method for testing the two-spot moth, is characterized in that: mainly comprises the following steps:

[0054] (1) Choose a fresh-keeping box with a diameter of 16cm and a height of 9cm, put a filter paper with a slightly smaller diameter in it, and drip water on the filter paper to make the filter paper moist;

[0055] (2) Transfer the eggs laid by adults of Spodoptera spp. in the same period of time to the fresh-keeping box described in step (1). After hatching the larvae, transfer each larvae to a container with a diameter of 3 cm and a height of 8 cm. Single-head feeding with synthetic feed blocks in glass tubes;

[0056] (3) After feeding for 20-24 days, the larvae are nearly mature and pupate, and no feed is added; transfer the pupae to a sterilized petri dish with a diameter of 6 cm and a height of 1 cm, with 10 male and 10 larvae in each dish;

[0057] (4) Put the petri dish with pupae in step (3) into a glass bottle with a diamete...

Embodiment 3

[0066] A kind of artificial breeding method for testing the two-spot moth, is characterized in that: mainly comprises the following steps:

[0067] (1) Choose a fresh-keeping box with a diameter of 18cm and a height of 10cm, put a filter paper with a slightly smaller diameter in it, and drip water on the filter paper to make the filter paper moist;

[0068] (2) Transfer the eggs laid by adults of Spodoptera spp. within the same period of time to the fresh-keeping box described in step (1). After hatching the larvae, transfer each larvae to a container with a diameter of 4 cm and a height of 9 cm. Single-head feeding with synthetic feed blocks in glass tubes;

[0069] (3) After feeding for 20-24 days, the larvae are nearly mature and pupate, and no feed is added; the pupae are transferred to a sterilized petri dish with a diameter of 6 cm and a height of 1 cm, with 10 male and 10 larvae in each dish;

[0070] (4) Put the petri dish with pupae in step (3) into a glass bottle wi...

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Abstract

The invention discloses a method for artificially rearing athetis lepigone for test. According to the method for artificially rearing the athetis lepigone for test, artificially synthesized fodder is used for feeding the athetis lepigone, the method for artificially rearing the athetis lepigone for test is adopted in a matching mode at the same time, the survival rate of the athetis lepigone, the pupation rate of the athetis lepigone, the eclosion rate of the athetis lepigone and the egg laying amount of single female athetis lepigone are improved greatly, and a favorable condition is offered to the regular supply of the athetis lepigone for test. Meanwhile, the sensitivity of the athetis lepigone to a test is improved, and therefore the accuracy rate and the realness of a biotic experiment are improved. Moreover, according to the method for artificially rearing the athetis lepigone for test, due to the fact that the artificially synthesized fodder is adopted, the athetis lepigone is reared without the limitation of seasons, and rearing consistency is improved.

Description

technical field [0001] The invention relates to a method for artificially raising insects for testing, in particular to a method for artificially raising insects for testing. Background technique [0002] Two-point moth (Proxenus lepigone Moschler) belongs to the Lepidoptera family Noctuidae of the order Lepidoptera, and belongs to the genus of the moth. It is a new corn pest in recent years. It is reported to be distributed in Japan, North Korea, Siberia and other Asian regions and Sweden, Finland and other European regions. In July 2005, China firstly reported the damage to corn in Hebei Province, and then successively occurred in Shandong, Henan, Anhui, Jiangsu, Shanxi, Beijing and other provinces and cities. There is a trend of further increase and aggravation. In 2011, the second-point committee armyworm broke out in the summer corn area of ​​Huanghuaihai, posing a serious threat to the safe production of summer corn. Spodoptera moth has a variety of feeding habits an...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K1/14A23K1/18
Inventor 李丽莉李召波于毅张安盛门兴元周仙红庄乾营翟一凡
Owner INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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